Sequence and Sequence Analysis of E1 and pIX Regions of the BAV3 Genome

Elgadi, Mabrouk; Rghei, Nezar; Haj-Ahmad, Yousef

doi:10.1159/000150329

Cited by 15 publications

(10 citation statements)

References 15 publications

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“…0 and 13.7. This sequence was compared with the similar sequences reported for HAd5 [25], bovine adenovirus type 3 (BAd3) [26][27][28] and PAd4 [29]. On the basis of protein homology, ORFs homologous to HAd5 E1A, E1B-19k, E1B-55k, pIX, pIVa2 and two novel ORFs [203 and 208 amino acid residues (R)] were identified in the PAd3 genome ( fig.…”

Section: Resultsmentioning

confidence: 99%

Sequence Analysis of Porcine Adenovirus Type 3 E1 Region, pIX and pIVa2 Genes, and Two Novel Open Reading Frames

Aggarwal¹,

Mittal²

2000

Intervirology

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The porcine adenovirus type 3 (PAd3) genome between map units 0 and 13.7 was sequenced and compared with similar regions of other adenoviruses. This region consists of the left inverted terminal repeat sequences involved in DNA packaging, the entire early region 1 (E1) and the protein IX (pIX) transcription unit. The lower strand contains the C-terminal end of IVa2 of the E2A transcriptional unit and two novel open reading frames (ORFs). The E1 transcription unit consists of ORFs for proteins homologous to the E1A, E1B-17k and E1B-55k of both human adenovirus type 5 (HAd5) and bovine adenovirus type 3 (BAd3). The predicted PAd3 pIX demonstrated homology with the N-terminal portion of the pIXs of HAd5 and BAd3. On the lower strand, immediately after the putative IVa2 ORF, there are two unique ORFs of 208 and 203 amino acid residues that showed homology with Epstein-Barr virus nuclear antigens and other cellular transcription factors.

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Section: Resultsmentioning

confidence: 99%

Sequence Analysis of Porcine Adenovirus Type 3 E1 Region, pIX and pIVa2 Genes, and Two Novel Open Reading Frames

Aggarwal¹,

Mittal²

2000

Intervirology

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“…Sequencing analyses of the BAV3 E1 and pIX regions [9], BAV2 E1 region [10], BAV3 E3 [11], and BAV2 E3 [12] have provided evidence that the genomes of BAVs have similar genetic organizations to those of the well-characterized human Ads.…”

Section: Introductionmentioning

confidence: 99%

Sequencing Analysis of the Region Encoding the DNA Polymerase of Bovine Adenovirus Serotypes 2 and 3

et al. 1998

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Bovine adenoviruses (BAVs) are important pathogens causing significant economic losses to the cattle industry. We have been interested in the differences among serotypes of these viruses, particularly in their pathogenicity and host range. As part of our efforts to better understand these viruses, we have determined the nucleotide sequences for serotype 3 (BAV3) at map coordinates beween 11.7 and 23.7% and for serotype 2 (BAV2) between 13.1 and 24.0%. Analyses of these sequences revealed large open reading frames (ORFs) encoded within the leftward-reading strand of the viral DNA. The coding capacity of the ORF in BAV3 is 1,167 amino acid residues and 1,138 in BAV2. A search in the GenEMBL protein sequence databank for homology to the predicted polypeptide products of these ORFs established their identity as that for the adenovirus (Ad) DNA polymerase (DNA pol). The deduced polypeptide sequences were aligned with each other and with other known Ad DNA pols to reveal regions of homology and similarity. The comparison at the amino acid sequence level not only showed that the bovine Ad DNA pols from the two serotypes are quite distinct from each other, but also revealed that Ad DNA pols contain multiple domains that are highly conserved among human, canine and bovine Ads. These conserved domains are likely important for the multiple functions attributed to Ad DNA pol, which include catalysis of its own initiation complex, elongation of nascent DNA strand, as well as correction of DNA replication errors.

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“…We have previously reported the preliminary structur al characterization of the genomes of BAV2 and BAV3 as to their cloning and restriction enzyme analysis. Sequenc ing analyses of the BAV3 El and pIX regions [5,6], BAV3 E3 region [7. 8], BAV2 El and pIX regions [9], BAV2 E3 [10] and E4 regions [11], and their DNA polymerases (unpubl.…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis of the Terminal Protein Precursor Coding Regions from Bovine Adenovirus Serotypes 2 and 3

et al. 1997

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As part of our efforts to develop bovine adenoviruses into a vector system, we sequenced the region predicted to encode the terminal protein precursor of either bovine adenoviruses type 2 and bovine adenoviruses type 3. We examined the regions containing the terminal protein precursors and showed that the bovine adenovirus early region 2B had an identical organization to all adenoviruses so far examined. The bovine adenovirus terminal protein precursors and those of other adenoviruses were also compared in a sequence alignment analysis and several highly conserved structural domains were identified. Finally, we showed how the various terminal protein precursors were related in a sequence pair similarity analysis and concluded that the terminal protein precursors of bovine adenoviruses types 2 and 3 are significantly different from each other at the protein sequence level.

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Sequence and Sequence Analysis of E1 and pIX Regions of the BAV3 Genome

Cited by 15 publications

References 15 publications

Sequence Analysis of Porcine Adenovirus Type 3 E1 Region, pIX and pIVa2 Genes, and Two Novel Open Reading Frames

Sequence Analysis of Porcine Adenovirus Type 3 E1 Region, pIX and pIVa2 Genes, and Two Novel Open Reading Frames

Sequencing Analysis of the Region Encoding the DNA Polymerase of Bovine Adenovirus Serotypes 2 and 3

Sequence Analysis of the Terminal Protein Precursor Coding Regions from Bovine Adenovirus Serotypes 2 and 3

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