Sequence, biochemical characteristics and expression of a novel Sigma-class of glutathione S-transferase from the intertidal copepod, Tigriopus japonicus with a possible role in antioxidant defense

Lee, Young-Mi; Lee, Kyun-Woo; Park, Hyun; Park, Heum Gi; Raisuddin, Sheikh; Ahn, In-Young; Lee, Jae‐Seong

doi:10.1016/j.chemosphere.2007.05.087

Cited by 69 publications

(19 citation statements)

References 48 publications

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“…Exposure to Cu and Cd was shown to induce the expression of GST transcripts in many aquatic organisms (Yoshinaga et al, 2007;Lee et al, 2008;Kim et al, 2010). For example, expression of Tigriopus japonicus and Takifugu obscurus GSTs were signifi cantly induced by Cd and Cu exposure (Lee et al, 2007(Lee et al, , 2008Kim et al, 2010 (Bryan and Hummerstone, 1971). A signifi cant inhibition of GST activity was observed in Tubifex tubifex and Cyprinus carpio after exposure to 50-250 μg/L of Cu (Dautrememepuits et al, 2002;Mosleh et al, 2005).…”

Section: Discussionmentioning

confidence: 91%

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Zhang

Liu

et al. 2011

Chin. J. Ocean. Limnol.

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Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxifi cation of xenobiotics, and also play important roles in antioxidant defense. We identifi ed two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profi les of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these fi ndings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.

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Section: Discussionmentioning

confidence: 91%

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Zhang

Liu

et al. 2011

Chin. J. Ocean. Limnol.

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“…Dehydration caused a late induction of an Arabidopsis GST (Atgstu8) and that was attributed to secondary oxidative stress effects (Bianchi et al, 2002). Recently, a sigma-class of GST (GST-S) from Tigriopus japonicus was shown to play a potential role against antioxidative stress (Lee et al, 2007).…”

Section: Analysis Of Pjgstu1 Transgenic Tobacco Plantsmentioning

confidence: 99%

A chloroplast-localized and auxin-induced glutathione S-transferase from phreatophyte Prosopis juliflora confer drought tolerance on tobacco

George

Venkataraman

Parida

2010

Journal of Plant Physiology

103

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“…In invertebrates, most studies have demonstrated that glutathione S-transferases (GSTs) function in antioxidant defense systems and the detoxification of insecticides and xenobiotics are often induced, e.g., after exposure to toxins [43][44][45][46][47]. The third stress-related gene examined in this study, tsp was up-regulated by DEP and DPP and highly sensitive to DPP (Figures 1 and 2).…”

Section: Effects Of Three Paes On the Expression Of Stress-related Genesmentioning

confidence: 81%

Differential Expression of Physiological Genes of Neocaridina Denticulate at the Transcriptional Level Following Short-Term Exposure to Non-Lethal Concentrations of Phthalate Esters

Ch¹,

Tsai²,

Sung³

2017

J Pollut Eff Cont

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Xenobiotic phthalate esters (PAEs) are considered endocrine disrupting chemicals and are known to cause immunotoxicity, having been linked with the susceptibility of shrimp to pathogens; however, the effect on other physiological functions has not been explored. This study sought to understand the genetic responses of Neocaridina denticulate, a common freshwater shrimp, to three different PAEs, i.e., diethyl phthalate (DEP), dipropyl phthalate (DPrP), and diphenyl phthalate (DPP). The differential expression of 10 functional known expressed sequence tags derived from N. denticulate were analyzed by semi-quantitative RT-PCR after 24 h of exposure to non-lethal concentrations (0, 0.1, 1.0, and 10 mg/L) of PAEs in pond water. Compared with the control group, nine genes were differentially expressed in the DEP-treated groups, seven genes were differentially expressed in the DPrPtreated groups, and five genes were differentially expressed in the DPP-treated groups. These PAE-affected genes primarily belonged to three functional classes: defense-related genes (qm: QM protein hc: hemocyanin), metabolism-related genes (cathepsin-L like cysteine protease, catl; glutamate dehydrogenase, gdh; and inosine monophosphate dehydrogenase 1, impdh-1) and stress-related genes (hsp70: 70 kDa-heat shock protein; gst: glutathione S-transferase, and tps: trehalose-6-phosphate synthase). Among these, the stress-related genes were significantly up-regulated by DEP, DPrP, and DPP. These effects of PAEs on the expression of genes required for multiple physiological functions suggest that even with non-lethal concentrations of PAEs, a polluted aquatic environment may still present a potential risk to N. denticulate.

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Sequence, biochemical characteristics and expression of a novel Sigma-class of glutathione S-transferase from the intertidal copepod, Tigriopus japonicus with a possible role in antioxidant defense

Cited by 69 publications

References 48 publications

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

A chloroplast-localized and auxin-induced glutathione S-transferase from phreatophyte Prosopis juliflora confer drought tolerance on tobacco

Differential Expression of Physiological Genes of Neocaridina Denticulate at the Transcriptional Level Following Short-Term Exposure to Non-Lethal Concentrations of Phthalate Esters

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