Sequence of Genome Segments 1, 2, and 3 of the Grass Carp Reovirus (Genus Aquareovirus, Family Reoviridae)

Qin, Fang; Attoui, Houssam; Biagini, Jean François Philippe; Zhang, Zhu; Micco, Philippe de; Lamballerie, Xavier de

doi:10.1006/bbrc.2000.3215

Cited by 84 publications

(38 citation statements)

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“…Of the three types of GCRV, GCRV-873 was the first fish virus to be characterized and sequenced [13] and was used as the target in early studies focusing on disease-resistant breeding and virus-host interactions. However, recent studies showed that most of the GCRV isolated in Southern China are type II GCRVs, such as GCRV-HZ08, GCRV-GD108 and GCReV-109 [10–12].…”

Section: Introductionmentioning

confidence: 99%

Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

Zhang

Pei

et al. 2017

BMC Genomics

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BackgroundGrass carp is an important farmed fish in China that is affected by serious disease, especially hemorrhagic disease caused by grass carp reovirus (GCRV). The mechanism underlying the hemorrhagic symptoms in infected fish remains to be elucidated. Although GCRV can be divided into three distinct subtypes, differences in the pathogenesis and host immune responses to the different subtypes are still unclear. The aim of this study was to provide a comprehensive insight into the grass carp response to different GCRV subtypes and to elucidate the mechanism underlying the hemorrhagic symptoms.ResultsFollowing infection of grass carp, GCRV-I was associated with a long latent period and low mortality (42.5%), while GCRV-II was associated with a short latent period and high mortality (81.4%). The relative copy number of GCRV-I remained consistent or decreased slightly throughout the first 7 days post-infection, whereas a marked increase in GCRV-II high copy number was detected at 5 days post-infection. Transcriptome sequencing revealed 211 differentially expressed genes (DEGs) in Group I (66 up-regulated, 145 down-regulated) and 670 (386 up-regulated, 284 down-regulated) in Group II. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed significant enrichment in the terms or pathways involved in immune responses and correlating with blood or platelets. Most of the DEGs in Group I were also present in Group II, although the expression profiles differed, with most DEGs showing mild changes in Group I, while marked changes were observed in Group II, especially the interferon-related genes. Many of the genes involved in the complement pathway and coagulation cascades were significantly up-regulated at 7 days post-infection in Group II, suggesting activation of these pathways.ConclusionGCRV-I is associated with low virulence and a long latent period prior to the induction of a mild host immune response, whereas GCRV-II is associated with high virulence, a short latent period and stimulates a strong and extensive host immune response. The complement and coagulation pathways are significantly activated at 7 days post-infection, leading to the endothelial cell and blood cell damage that result in hemorrhagic symptoms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3824-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

Zhang

Pei

et al. 2017

BMC Genomics

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“…The 11 genome segments encode seven structural proteins (VP1-VP7) and five nonstructural proteins [7,8]. Although the 11-part segmented genome of GCRV is similar in composition to members of the genus Rotavirus within the family Reoviridae , there is no genetic relationship with rotavirus based on reciprocal RNA-RNA dot blot hybridization [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Recent cryo-electron microscopy (cryo-EM) and three-dimensional (3D) structural reconstruction images indicate that the inner layer arranges with a T = 1 symmetry. This layer is composed of 5 proteins (including VP1-VP4 and VP6) and possesses the enzymatic activities necessary for viral transcription [6,7,13,14]. The other outer capsid proteins, arranged on an incomplete T = 13 icosahedral lattice, are composed of VP5 and VP7; each GCRV virion contains 200 trimers formed by VP5-VP7 heterodimers, a structure homologous to the μ1 3 σ3 3 complex of MRV (Mammalian reovirus)[13,15].…”

Section: Introductionmentioning

confidence: 99%

Antibodies against outer-capsid proteins of grass carp reovirus expressed in E. coli are capable of neutralizing viral infectivity

Shao

Sun

Qin

2011

Virol J

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BackgroundGrass carp reovirus (GCRV), which causes severe infectious outbreaks of hemorrhagic disease in aquatic animals, is a highly pathogenic agent in the Aquareovirus genus of family Reoviridae. The outer capsid shell of GCRV, composed of the VP5-VP7 protein complex, is believed to be involved in cell entry. The objective of this study was to produce a major neutralization antibody for mitigating GCRV infection.ResultsRecombinant plasmids of GCRV outer capsid proteins VP5 and VP7 were constructed and expressed in prokaryotic cells in our previous work. In this study, we prepared GCRV Antibody (Ab), VP5Ab and VP7Ab generated from purified native GCRV, recombinant VP5 and VP7 respectively. Immunoblotting analysis showed that the prepared antibodies were specific to its antigens. In addition, combined plaque and cytopathic effect (CPE)-based TCID50 (50% tissue culture infective dose) assays showed that both VP5Ab and VP7Ab were capable of neutralizing viral infectivity. Particularly, the neutralizing activity of VP7Ab was 3 times higher than that of VP5Ab, suggesting that VP7 might be a dominating epitope. Moreover, the combination of VP5Ab and VP7Ab appeared to enhance GCRV neutralizing capacity.ConclusionsThe results presented in this study indicated that VP7 protein was the major epitope of GCRV. Furthermore, VP5Ab and VP7Ab in combination presented an enhanced capacity to neutralize the GCRV particle, suggesting that the VP5 and VP7 proteins may cooperate with each other during virus cell entry. The data can be used not only to further define the surface epitope domain of GCRV but may also be applicable in the designing of vaccines.

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“…Some GCRV isolates were examined in the present study, which were identified in previous studies (Mohd Jaafar et al 2008; Rangel et al 1999; Fang et al 2000; Su et al 2010; Attoui et al 2002; Fan et al 2010). Table 1 presents information about the specific names of twenty five GCRV isolates, their abbreviations, locations where they were collected, the genes of GCRV and their GenBank accession numbers.…”

Section: Methodsmentioning

confidence: 95%

“…So far, some gene sequences of GCRV isolates have been reported (Mohd Jaafar et al 2008; Rangel et al 1999; Fang et al 2000; Su et al 2010; Attoui et al 2002; Fan et al 2010). vp4 , vp6 and vp7 gene in GCRV encode major outer capsid proteins and are conservative.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of newly isolated grass carp reovirus

et al. 2014

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Grass carp reovirus (GCRV) is a causative agent of haemorrhagic disease in grass carp that drastically affects grass carp aquaculture. Here we report a novel GCRV isolate isolated from sick grass carp that induces obvious cytopathic effect in CIK cells and name it as GCRV096. A large number of GCRV 096 viral particles were found in the infected CIK cells by electron microscope. The shape, size and the arrangement of this virus were similar to those of grass carp reovirus. With the primers designed according to GCRV 873 genome sequences, specific bands were amplified from sick grass carp and the infected CIK cells. The homology rates among vp4, vp6 and vp7 gene in GCRV 096 and those of some GCRV isolates were over 89%. In this study, the sequences of vp4, vp6 and vp7 were used to analyse sequence variation, phylogenetic relationships and genotypes in twenty five GCRV isolates. The results indicated these twenty five GCRV isolates should be attributed to four genotypes. And there were no obvious characteristics in the geographical distribution of GCRV genotype. The study should provide the exact foundation for developing more effective prevention strategies of grass carp haemorrhagic disease.

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Sequence of Genome Segments 1, 2, and 3 of the Grass Carp Reovirus (Genus Aquareovirus, Family Reoviridae)

Cited by 84 publications

References 9 publications

Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

Antibodies against outer-capsid proteins of grass carp reovirus expressed in E. coli are capable of neutralizing viral infectivity

Phylogenetic analysis of newly isolated grass carp reovirus

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