Sequence of the open reading frame of the <i>FL01</i> gene from <i>Saccharomyces cerevisiae</i>

Teunissen, Aloys; Holub, E.; Hucht, J. Van Der; Berg, Johan; Steensma, H. Yde

doi:10.1002/yea.320090413

Cited by 110 publications

(103 citation statements)

References 19 publications

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“…The MS group corresponds to the Flo1 phenotype, while the GMS group corresponds to the NewFlo phenotype (reviewed by Stratford, 1992). The FLO1 gene is one constituent of the Flo1 phenotype (Teunissen et al, 1993;Watari et al, 1994). It codes for an integral membrane protein with protruding N-terminal actins as a cell surface lectin.…”

Section: Discussionmentioning

confidence: 99%

Constitutive Flocculation inSaccharomyces cerevisiae Through Overexpression of theGTS1 Gene, coding for a ‘Glo’-type Zn-finger-containing Protein

Bossier

Goethals

Rodrigues-Pousada

1997

Yeast

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Section: Discussionmentioning

confidence: 99%

Constitutive Flocculation inSaccharomyces cerevisiae Through Overexpression of theGTS1 Gene, coding for a ‘Glo’-type Zn-finger-containing Protein

Bossier

Goethals

Rodrigues-Pousada

1997

Yeast

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“…9,[12][13][14] The five FLO genes, FLO1, FLO5, FLO9, FLO10, and FLO11, have been described in S. cerevisiae thus far. [15][16][17][18][19] The FLO1, 5, 9, and 10 genes confer cell-cell adhesion (flocculation) ability, whereas FLO11 is responsible for substrate adhesion. 20) FLO1, FLO5, and FLO9 are highly similar to each other, but FLO10 has lower similarity.…”

mentioning

confidence: 99%

Construction of FlocculentKluyveromyces marxianusStrains Suitable for High-Temperature Ethanol Fermentation

Nonklang

Ano²,

Abdel-Banat

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Flocculating yeasts are highly useful in fermentation processes because these cells can be separated easily from the fermentation mash. However, native yeasts are usually non-flocculating, including Kluyveromyces marxianus, which exhibits a potent high-temperature ethanol fermentation ability. We describe here the construction of flocculent K. marxianus strains via the introduction of the FLO1, FLO5, FLO9, and FLO10 genes from Saccharomyces cerevisiae. The S. cerevisiae FLO genes were overexpressed by upstream insertion of the constitutive TDH3 promoter, resulting in flocculent S. cerevisiae strains. These TDH3p-FLO sequences were then amplified by PCR and introduced directly into a K. marxianus strain. These K. marxianus strains showed a flocculation phenotype, indicating that the introduced S. cerevisiae TDH3 promoter and all FLO genes were functional in this strain. Moreover, a flocculating K. marxianus strain showed the same ethanol production profile as that of its wild-type parent. The K. marxianus flocculating strains we generated should be useful in the future development of cost-effective fed-batch and continuous fermentation systems at high temperatures.Key words: Kluyveromyces marxianus; yeast; flocculation; ethanol fermentationFlocculation is an attractive property in industrial yeasts because the cells can be easily separated from a fermentation mash. The application of flocculating strains to industrial fermentation processes removes the need for extensive and costly cell separation equipment, and these yeasts are thus suitable for both fedbatch and continuous fermentation. [1][2][3][4] In addition, flocculating yeast cells are useful in immobilized cell systems with no inert support materials. 5) However, although flocculation mechanisms and the introduction of this property to non-flocculent yeast strains have been extensively studied in S. cerevisiae, few studies of this nature have been undertaken in other yeast species. 6,7) The mechanism of yeast flocculation involves the interaction of lectin-like proteins, called flocculins, with receptors on a neighboring cell wall, resulting in the formation of aggregates. [8][9][10][11] Environmental factors such as pH, calcium, and organic stress also affect flocculation. 9,[12][13][14] The five FLO genes, FLO1, FLO5, FLO9, FLO10, and FLO11, have been described in S. cerevisiae thus far. [15][16][17][18][19] The FLO1, 5, 9, and 10 genes confer cell-cell adhesion (flocculation) ability, whereas FLO11 is responsible for substrate adhesion. 20) FLO1, FLO5, and FLO9 are highly similar to each other, but FLO10 has lower similarity. The mechanisms of flocculation in other yeasts appear to be lectin-based but of differing specificities. For instance, galactose and its derivatives have been found to inhibit flocculation of K. bulgaricus and K. lactis, suggesting that this interaction involves a galactose-specific lectin. 21,22) The advantages of using flocculating yeast cells in fermentation processes have led to many studies on the construction of flocculent s...

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“…1C). All described features are common to other cell-wall proteins found in other fungal species (Lipke et al, 1989;Lu et al, 1994;Moukadiri et al, 1997;Roy et al, 1991;Teunissen et al, 1993;van der Vaart et al, 1995). The difference between the predicted size of CaSsr1p (22?5 kDa) and that deduced from the mobility in SDS-PAGE (70 kDa) could be accounted for by Oglycosylation and GPI modification (Fig.…”

Section: Structural Analysis Of the Amino Acid Sequence Encoded By Camentioning

confidence: 58%

Identification and study of a Candida albicans protein homologous to Saccharomyces cerevisiae Ssr1p, an internal cell-wall protein

et al. 2003

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After screening of a Candida albicans genome database, the product of an ORF (IPF 3054) that has 62 % homology with Saccharomyces cerevisiae Ssr1p, an internal cell-wall protein, was identified and named CaSsr1p. The deduced amino acid sequence shows that CaSsr1p contains an N-terminal hydrophobic signal peptide, is rich in Ser and Thr amino acids and has a potential glycosylphosphatidylinositol-attachment signal. CaSsr1p is released following degradation of isolated cell walls by zymolyase (mainly a 1,3-b-glucanase) and therefore seems to be covalently linked to the b-glucan of the cell walls. Both disruption and overexpression of the CaSSR1 gene caused an increased sensitivity to calcofluor white, Congo red and zymolyase digestion. These results suggest that CaSsr1p has a structural role associated with the cell-wall b-glucan. INTRODUCTIONCandida albicans is an opportunistic pathogenic fungus in humans which can cause either septicaemic or mucosal infections (Odds, 1988(Odds, , 1994. The number of fungal infections caused by C. albicans has increased dramatically in the last few decades, due to the rise in the number of immunocompromised patients and their life expectancies (Fox, 1993). C. albicans is a dimorphic organism capable of reproducing by budding (yeast cells) or by producing germ tubes (mycelial cells) depending upon environmental factors (Odds, 1988); this morphological transition has been associated with pathogenicity (Calderone & Braun, 1991;Odds, 1988;Sentandreu et al., 1993). Because the cell wall of C. albicans is the fungal structure responsible for the initial interaction with the host and for the characteristic shape of each growth form, several studies have focused on its biosynthesis and function (Gozalbo et al., 1994;Sentandreu et al., 1993;Valentin et al., 2000).The cell wall of C. albicans is a complex biochemical entity composed mainly of three components, namely, glucans (1,3-b-and 1,6-b-glucan), mannoproteins and chitin (Fleet, 1991;Valentin et al., 2000). The b-glucans are the main components, accounting for 50-60 % by weight of the cell wall in C. albicans and other fungi. Chitin, a linear polymer of 1,4-b-linked N-acetylglucosamine units, is a relatively minor (1-10 %) but important constituent. In most fungi, b-glucans and chitin polymers account for the rigidity of the cell wall as well as its morphology . Mannoproteins represent 30-40 % of the total cell wall and determine the surface properties, enabling C. albicans cells to interact and adhere to host tissues (Chaffin et al., 1998).Cell-wall mannoproteins can be divided into three groups according to the methods used for their extraction. One group can be solubilized by detergents, such as SDS, or chaotropic agents, such as urea, and is formed by mannoproteins loosely associated with other components of the cell wall (Elorza et al., 1985;Valentin et al., 1984). The second group can be extracted by reducing agents, DTT or b-mercaptoethanol (Casanova et al., 1989;Orlean et al., 1986). The third group can be released from the ce...

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Sequence of the open reading frame of the FL01 gene from Saccharomyces cerevisiae

Cited by 110 publications

References 19 publications

Constitutive Flocculation inSaccharomyces cerevisiae Through Overexpression of theGTS1 Gene, coding for a ‘Glo’-type Zn-finger-containing Protein

Constitutive Flocculation inSaccharomyces cerevisiae Through Overexpression of theGTS1 Gene, coding for a ‘Glo’-type Zn-finger-containing Protein

Construction of FlocculentKluyveromyces marxianusStrains Suitable for High-Temperature Ethanol Fermentation

Identification and study of a Candida albicans protein homologous to Saccharomyces cerevisiae Ssr1p, an internal cell-wall protein

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