Sequence polymorphism of nematode effectors highlights molecular differences among the subspecies of the tobacco cyst nematode complex

Alenda, Cristina; Gallot-Legrand, Aurore; Fouville, Didier; Grenier, Éric

doi:10.1016/j.pmpp.2013.08.004

Cited by 14 publications

(22 citation statements)

References 33 publications

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“…After amplification of a fragment of the Cle5 gene and digestion of the PCR product by Mbo I, the restriction profiles obtained all showed a single band around 200 bp (data not shown) that matched with the expected molecular pattern observed for the Gtt subspecies while profiles obtained for the Gts and Gtv reference populations showed two additional lower bands (Alenda et al, 2013).…”

Section: Subspecies Status Of the Nematode Populationssupporting

confidence: 55%

“…In order to determine the subspecies status, DNA was extracted from one cyst per population using the procedure described in Alenda et al (2013). Briefly, 150 lL of a lysis buffer (200 mM tris-HCl, pH 8.5; 200 mM NaCl; 25 mM EDTA, 0.5% SDS) and 75 lL of sodium acetate (3 M, pH 5.2) were added to a crushed cyst and incubate for 10 min at 20°C.…”

Section: Subspecies Statusmentioning

confidence: 99%

“…A PCR was carried out in a final volume of 25 lL using the primers CAPS Cle5F and CAPS Cle5R (0.4 lmole each) (Alenda et al, 2013), 1.25 U Taq Goflexi (Promega, France), 5 lL Taq buffer (5Â), 0.2 nmole of each dNTPs and 3 mmole of MgCl 2 . PCR conditions were as follows: 95°C for 1 min, 30 cycles of 95°C for 30 s, 64°C for 50 s and 72°C for 60 s, ended by a final elongation step at 72°C during 5 min.…”

Section: Subspecies Statusmentioning

confidence: 99%

“…PCR conditions were as follows: 95°C for 1 min, 30 cycles of 95°C for 30 s, 64°C for 50 s and 72°C for 60 s, ended by a final elongation step at 72°C during 5 min. These PCR products were then digested by Mbo I and the restriction fragments were revealed by electrophoresis on a 1% TBE-buffered agarose gel stained with SYBRsafe (Alenda et al, 2013).…”

Section: Subspecies Statusmentioning

confidence: 99%

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Human influence on the dispersal and genetic structure of French Globodera tabacum populations

Alenda

Montarry

Grenier

2014

Infection, Genetics and Evolution

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Section: Subspecies Status Of the Nematode Populationssupporting

confidence: 55%

Section: Subspecies Statusmentioning

confidence: 99%

Section: Subspecies Statusmentioning

confidence: 99%

Section: Subspecies Statusmentioning

confidence: 99%

See 2 more Smart Citations

Human influence on the dispersal and genetic structure of French Globodera tabacum populations

Alenda

Montarry

Grenier

2014

Infection, Genetics and Evolution

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“…Notably, nematodes often produce/secrete CLE effectors with different CLE motifs (Lu et al, 2009;Wang et al, 2011;Alenda et al, 2013). Based on studies of the Arabidopsis-BCN pathosystem (Replogle et al, , 2013 as well as a recent finding that the nonmodified GrCLE1-1 peptide could bind physically to BARELY ANY MERISTEM1 (BAM1) and BAM2 of Arabidopsis , it is likely that PCN and other cyst nematodes may utilize their secreted CLE effectors to activate multiple CLE signaling pathways through different host receptors to promote successful infection.…”

Section: Discussionmentioning

confidence: 99%

In Planta Processing and Glycosylation of a Nematode CLAVATA3/ENDOSPERM SURROUNDING REGION-Like Effector and Its Interaction with a Host CLAVATA2-Like Receptor to Promote Parasitism

Chen

Lang²,

Chronis

et al. 2014

Plant Physiol.

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Like other biotrophic plant pathogens, plant-parasitic nematodes secrete effector proteins into host cells to facilitate infection. Effector proteins that mimic plant CLAVATA3/ENDOSPERM SURROUNDING REGION-related (CLE) proteins have been identified in several cyst nematodes, including the potato cyst nematode (PCN); however, the mechanistic details of this crosskingdom mimicry are poorly understood. Plant CLEs are posttranslationally modified and proteolytically processed to function as bioactive ligands critical to various aspects of plant development. Using ectopic expression coupled with nanoliquid chromatography-tandem mass spectrometry analysis, we show that the in planta mature form of proGrCLE1, a multidomain CLE effector secreted by PCN during infection, is a 12-amino acid arabinosylated glycopeptide (named GrCLE1-1Hyp4,7g) with striking structural similarity to mature plant CLE peptides. This glycopeptide is more resistant to hydrolytic degradation and binds with higher affinity to a CLAVATA2-like receptor (StCLV2) from potato (Solanum tuberosum) than its nonglycosylated forms. We further show that StCLV2 is highly up-regulated at nematode infection sites and that transgenic potatoes with reduced StCLV2 expression are less susceptible to PCN infection, indicating that interference of the CLV2-mediated signaling pathway confers nematode resistance in crop plants. These results strongly suggest that phytonematodes have evolved to utilize host cellular posttranslational modification and processing machinery for the activation of CLE effectors following secretion into plant cells and highlight the significance of arabinosylation in regulating nematode CLE effector activity. Our finding also provides evidence that multidomain CLEs are modified and processed similarly to single-domain CLEs, adding new insight into CLE maturation in plants.

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Occurrence of the tobacco cyst nematode subspecies Globodera tabacum subsp. virginiae in France

2015

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Tobacco cyst nematode is a sexually diploid species composed of three subspecies, Globodera tabacum subsp. tabacum (Gtt), G. tabacum subsp. virginiae (Gtv) and G. tabacum subsp. solanacearum (Gts). In Europe, only Gtt have been reported to date. Here, we report from a plant in a French tobacco field, the identification of a mixture between cysts of the subspecies Gtt and cysts genetically different. The objective of the present study was to determine the subspecies status of those particular cysts. To this aim, we performed CAPS analyses on two genes and a principal component analysis on microsatellite data. The results obtained with both CAPS and microsatellite markers demonstrate for the first time the occurrence of G. tabacum subsp. virginiae in France.

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Sequence polymorphism of nematode effectors highlights molecular differences among the subspecies of the tobacco cyst nematode complex

Cited by 14 publications

References 33 publications

Human influence on the dispersal and genetic structure of French Globodera tabacum populations

Human influence on the dispersal and genetic structure of French Globodera tabacum populations

In Planta Processing and Glycosylation of a Nematode CLAVATA3/ENDOSPERM SURROUNDING REGION-Like Effector and Its Interaction with a Host CLAVATA2-Like Receptor to Promote Parasitism

Occurrence of the tobacco cyst nematode subspecies Globodera tabacum subsp. virginiae in France

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