Sequence-specific identification of 18 pathogenic microorganisms using microarray technology

Wilson, W J; Strout, Cheryl L.; DeSantis, Todd Z.; Stilwell, Jackie L.; Carrano, A.V.; Andersen, Gary L.

doi:10.1006/mcpr.2001.0397

Cited by 251 publications

(156 citation statements)

References 17 publications

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“…The use of a random PCR step obviates the need for a priori knowledge of the infectious agent and thus identification is limited only by the spectrum of viral probes present on the array. This is in contrast to conventional RT-PCR-based detection schemes (2,21), wherein the outcome is necessarily restricted by the initial selection of targets and corresponding primers. Even when degenerate multiplex RT-PCR is used, the range of target viruses remains narrow.…”

Section: Discussionmentioning

confidence: 99%

Microarray-based detection and genotyping of viral pathogens

Wang

Coscoy

Zylberberg

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

694

243

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The detection of viral pathogens is of critical importance in biology, medicine, and agriculture. Unfortunately, existing techniques to screen for a broad spectrum of viruses suffer from severe limitations. To facilitate the comprehensive and unbiased analysis of viral prevalence in a given biological setting, we have developed a genomic strategy for highly parallel viral screening. The cornerstone of this approach is a long oligonucleotide (70-mer) DNA microarray capable of simultaneously detecting hundreds of viruses. Using virally infected cell cultures, we were able to efficiently detect and identify many diverse viruses. Related viral serotypes could be distinguished by the unique pattern of hybridization generated by each virus. Furthermore, by selecting microarray elements derived from highly conserved regions within viral families, individual viruses that were not explicitly represented on the microarray were still detected, raising the possibility that this approach could be used for virus discovery. Finally, by using a random PCR amplification strategy in conjunction with the microarray, we were able to detect multiple viruses in human respiratory specimens without the use of sequence-specific or degenerate primers. This method is versatile and greatly expands the spectrum of detectable viruses in a single assay while simultaneously providing the capability to discriminate among viral subtypes.

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Section: Discussionmentioning

confidence: 99%

Microarray-based detection and genotyping of viral pathogens

Wang

Coscoy

Zylberberg

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

694

243

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“…A multi pathogen identification microarray for 18 pathogenic prokaryotes, eukaryotes, and viruses was developed by Wilson et al [108]. The chip comprised of diagnostic regions of various pathogens and could identify pathogens in samples with high specificity.…”

Section: Detectionmentioning

confidence: 99%

Examination of animal and zoonotic pathogens using microarrays

Ojha¹,

Kostrzynska²

2007

Vet. Res.

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-The advancement in functional genomics, such as DNA microarrays along with the genome availability of important pathogens as well as of human and livestock species has allowed scientists to study the expression of thousands of genes in a single step. In the past decade, DNA arrays have been employed to study infectious processes of pathogens, in diagnostics, and to study host-pathogen interactions. The generation of enormous data sets by microarray experiments also stimulated the growth of a new generation of analytical software. The information provided by microarray experiments has been useful in generating new hypotheses for future research. The concept of DNA array technology has been utilized in the development of novel diagnostic methods. This review highlights the application of microarrays in the field of veterinary research.

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“…This is of interest for homeland security, public health, medicine, and veterinary diagnostics. For example, Wilson and collaborators developed a Multi-Pathogen Identification (MPID) microarray that identifies eighteen pathogenic prokaryotes, eukaryotes, and viruses amplifying unique regions of DNA from each microorganism, using the microarray to detect the presence or absence of pathogenspecific DNA sequences [3,4].…”

Section: Introductionmentioning

confidence: 99%

Infectious diseases detection by microarray: An overview of clinical relevant infections

Herrera-Rodríguez¹,

Elizondo-Quiroga²,

Álvarez-Maya³

2013

JBiSE

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Microarray technology is a powerful tool to investigate whole genome expression profiles to study the crosstalk between pathogens and associated hosts that cause illness. Microarrays have been used in several comparative genome hybridization studies of pathogens. In addition to detection and identification of pathogens, microarrays are ideal for characterizing genetic differences between bacteria isolates of the same species to the strain level. Furthermore, the use of microarrays has been gaining importance in the detection of viral agents. Here we explore the use of microarrays for simultaneous detection of viruses and modifications made over these techniques. Microarray technology has also been incorporated to compensate for time-consuming sequencing. The procedure of fungi identification based on sequences and studies reported the use of microarrays to identify pathogenic yeasts and molds by targeting the internal transcribed spacer regions in fungal rRNA genes. The remarkable advancement in genomics over the last decade has made it possible for microarray technology to evolve towards being the best option for clinical diagnostics because they have several advantages.

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Sequence-specific identification of 18 pathogenic microorganisms using microarray technology

Cited by 251 publications

References 17 publications

Microarray-based detection and genotyping of viral pathogens

Microarray-based detection and genotyping of viral pathogens

Examination of animal and zoonotic pathogens using microarrays

Infectious diseases detection by microarray: An overview of clinical relevant infections

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