Sequence type 38 Escherichia coli carrying bla
            CTX-M-14

Cheng, Peng; Zong, Zhiyong

doi:10.1099/jmm.0.028316-0

Cited by 7 publications

(6 citation statements)

References 13 publications

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“… 7 , 18 This study revealed that the majority of ST type with high antimicrobial resistant rate was ST38 which showed high sensitivity to piperacillin/tazobactam. According to the MLST results described by previous study, ST38 E. coli isolates were identified in China (CTX-M-14 producer), 19 Japan (CTX-M-9 and CTX-M-14 producers), 20 and France (OXA-48 producer). 21 Additionally, Rodríguez et al investigated MLST in bla CTX-M ESBL-producing E. coli from Germany, Netherlands, and UK.…”

Section: Discussionmentioning

confidence: 87%

Phenotypic and genotypic characterizations of extended-spectrum beta-lactamase-producing <em>Escherichia coli</em> in Thailand

Bubpamala¹,

Khuntayaporn²,

Thirapanmethee³

et al. 2018

IDR

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PurposeExtended-spectrum β-lactamases (ESBLs) have become an issue in community worldwide due to an increase in antibiotic resistance over the past decade. This study was aimed to investigate the phenotypic and genotypic characteristics of ESBL-producing Escherichia coli in Thailand.Materials and methodsIn this study, all clinical isolates collected from tertiary hospitals in Thailand were identified as E. coli by biochemical tests and MALDI-TOF mass spectrometry. ESBL-producing E. coli was preliminary screened with disk diffusion method by cephalosporin disks and confirmed by the method of combination disk diffusion. Antimicrobial susceptibility test was used to determine MIC values of all ESBL-producing E. coli. For genotypic detection, a variety of ESBL genes were determined by PCR. Moreover, multilocus sequence typing (MLST) analysis was performed on internal portions of seven housekeeping genes for the diversity and phylogenetic relatedness of E. coli clonal group.ResultsOf the 285 ESBL-producing E. coli, most were susceptible to carbapenems. These strains showed a high resistance rate to ciprofloxacin (85.26%). The most frequently detected gene was bla CTX-M1 group at about 71.23% followed by blaCTX-M9 group (38.95%). The blaTEM, bla PER, bla GES, bla VEB, and bla SHV genes were identified in 31.93%, 5.96%, 4.56%, 3.51%, and 0.70% of ESBL-producing isolates, respectively. The bla OXA-10 gene was detected in only one strain. ESBL-producing E. coli isolates with high antimicrobial resistance were further investigated. Among those, E. coli sequence type ST38 was mostly found, followed by ST405, ST410, and ST131. It is noteworthy that the blaCTX-M gene was mainly detected in all four ST-type E. coli clones (ST38, ST405, ST410, and ST131).ConclusionThis study provided a recent evidence of the genetic diversity of ESBL-producing E. coli in Thailand. In addition, the profile related to antimicrobial resistance pattern in this region was also demonstrated.

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Section: Discussionmentioning

confidence: 87%

Phenotypic and genotypic characterizations of extended-spectrum beta-lactamase-producing <em>Escherichia coli</em> in Thailand

Bubpamala¹,

Khuntayaporn²,

Thirapanmethee³

et al. 2018

IDR

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“…8 In addition this isolate belongs to the internationally widespread clone ST38, which is a worrisome evolving extraintestinal enteroaggregative E. coli clone. 10,30 Finally, one TEM-52 was identified in E. coli IV369-2321 from a Dutch dog on a 95 kb non-typeable plasmid. TEM-52 is usually associated with Tn3 transposon and represents the second most frequent ESBL type associated with resistance to third-generation cephalosporins in food-producing animals and in companion animals after CTX-M 19 and it has also been detected recently in companion animals clinical isolates.…”

Section: Discussionmentioning

confidence: 98%

Characterization of ESBL- and AmpC-Producing Enterobacteriaceae from Diseased Companion Animals in Europe

BogaertsPierre

HuangTe-Din

BouchahroufWarda

et al. 2015

Microbial Drug Resistance

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The study aimed to characterize beta-lactam resistance mechanisms of Enterobacteriaceae isolates recovered from diseased dogs and cats between 2008 and 2010 in a European surveillance program (ComPath I) for the antibiotic susceptibility of bacterial pathogens. A total of 608 non-duplicated Enterobacteriaceae isolates were obtained prior antibiotic treatment from diseased dogs (n=464) and cats (n=144). Among the 608 Enterobacteriaceae isolates, 22 presented a minimal inhibitory concentration against cefotaxime above EUCAST breakpoints of susceptibility. All the 22 isolates remained susceptible to carbapenems. Ten isolates were confirmed as extended-spectrum-beta-lactamase (ESBL) producers by PCR-sequencing of bla coding genes including 9 blaCTX-M (CTX-M-1, 14, 15, 32,…) and 1 blaTEM-52 and 12 were AmpC-producing isolates (10 plasmidic CMY-2 group and 2 isolates overexpressing their chromosomal AmpC). ESBLs and plasmid-mediated AmpC (pAmpC)-producing isolates were mainly recovered from dogs (n=17) suffering from urinary tract infections (n=13) and originated from eight different countries. ESBL-bearing plasmids were mostly associated with IncFII incompatibility groups while CMY-2 was predominantly associated with plasmid of the IncI1 group. ESBL/pAmpC-producing Escherichia coli belonged to phylogroup A (n=5), B2 (n=4), and D (n=5). Multilocus sequence typing analysis revealed that among three CTX-M-15-producing E. coli, two belong to sequence type (ST) 131 and one to ST405. The presence of CTX-M-15 including on IncFII plasmids in E. coli ST131-B2 has also been described in isolates of human origin. This suggests the possibility of exchanges of these isolates from humans to companion animals or vice-versa.

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“…Phylogenetic grouping performed by PCR (2) showed that these E. coli isolates belonged to group D, corresponding to extraintestinal and pathogenic strains. Multilocus sequence typing performed as described previously (10) showed that all three E. coli isolates belonged to ST38. ST38 E. coli isolates had previously been identified in China (CTX-M-14 producer) (10) and Japan (CTX-M-9 and CTX-M-14 producers) (14).…”

mentioning

confidence: 99%

“…Multilocus sequence typing performed as described previously (10) showed that all three E. coli isolates belonged to ST38. ST38 E. coli isolates had previously been identified in China (CTX-M-14 producer) (10) and Japan (CTX-M-9 and CTX-M-14 producers) (14). Pulsed-field gel electrophoresis analysis performed as described previously (1) showed that the three isolates were clonally related, indicating that the same clonal strain was circulating at least in Turkey and Egypt and was then imported into France.…”

mentioning

confidence: 99%