Sequence variability among ovine trophoblast interferon cDNA

Klemann, S W; Imakawa, Kazuhiko; Roberts, R. Michael

doi:10.1093/nar/18.22.6724

Cited by 29 publications

(17 citation statements)

References 4 publications

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“…Similar results are observed in the cellfree translation of poly(A)+ RNA extracted from day 14-16 ovine conceptuses [17]. Multiple oIFNτ cDNAs are then isolated and their nucleotide sequences characterized [29,30]. Southern blot analysis of ovine DNA has revealed numerous hybridizing bands representing multiple genes [30,32].…”

Section: Interferon As a Pregnancy Recognition Hormonesupporting

confidence: 71%

“…For type I IFN, at least three subtypes (α, β, ω) exist [24,25]; IFNα/ω and IFNβ are secreted by leukocytes and fibroblasts, respectively. IFNγ, on the other hand, is a type II IFN predominantly produced by T-cells [26] Subsequently, other oTP-1 cDNA isoforms and bovine counterparts have been isolated and characterized [19,29].…”

Section: Interferon As a Pregnancy Recognition Hormonementioning

confidence: 99%

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Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

Yamaguchi

Nagaoka

Matsuda

et al. 2001

Journal of Reproduction and Development

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Abstract. In order for pregnancy to be established, the maternal system must recognize the presence of a conceptus(es) within the uterus. This physiological phenomenon, which subsequently causes the extension of the corpus luteum (CL) life-span, is known as maternal recognition of pregnancy. In ruminant ungulates, interferon-tau (IFNτ), produced by the conceptus, binds to the receptor located in the maternal endometrium in a paracrine manner. Its binding then affects levels of endometrial oxytocin receptor and estrogen receptor, which reduces pulsatile secretion of a luteolysin, prostaglandin F2α, resulting in the extension of CL function. Production of IFNτ seems to parallel the degree of trophoblast elongation and as the conceptus begins its attachment to the endometrium, IFNτ levels decline rapidly. Involvement of IFNτ in the process of implantation has long been suspected, but a role for IFNτ other than CL extension or transcriptional regulation of IFNτ genes has not been elucidated. In fact, several transcription factors that regulate the expression of IFNτ have been found, but none could explain its unique regulation. Recent findings related to maternal recognition of pregnancy are presented herein and a new insight into the potential regulation of IFNτ is discussed.

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Section: Interferon As a Pregnancy Recognition Hormonesupporting

confidence: 71%

Section: Interferon As a Pregnancy Recognition Hormonementioning

confidence: 99%

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

Yamaguchi

Nagaoka

Matsuda

et al. 2001

Journal of Reproduction and Development

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“…The upstream promoters of five ov IFNT and two bo IFNT (including the one described above), whose cDNA have been previously cloned (13,26) contain the full Ets-2 binding sequence, with an intact GGAA core motif (Fig. 1 A).…”

Section: Resultsmentioning

confidence: 99%

“…The promoter and coding regions of IFNT were isolated from ov liver by PCR (30 cycles; 94°C͞50°C͞72°C, 1 min each). The primers corresponded to bp Ϫ428 to Ϫ408 (5Ј-AATACAAACATCAATATGGCC-3Ј) from boIFNT1 and bp ϩ677 to ϩ659 (5Ј-GCGAATTCG-CATCTTAGTCGGCAAGAG-3Ј) from the ovIFNT-p7 gene (13). The 3Ј-primer sequence is conserved in all identified ovIFNT genes.…”

Section: Methodsmentioning

confidence: 99%

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Control of interferon-τ gene expression by Ets-2

Ezashi

Ealy

Ostrowski

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

112

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Expression of the multiple interferon-(IFN-) genes is restricted to embryonic trophectoderm of ruminant ungulate species for a few days in early pregnancy. The promoter regions of these genes are highly conserved. A proximal (bp ؊91 to ؊69) sequence has been implicated in controlling trophoblast-specific expression. Here it was used as a target for yeast one-hybrid screening of a day 13 conceptus cDNA library. Two transcription factors of the Ets family, Ets-2 and GABP␣, were identified, consistent with the observation that active ovine IFN-genes contain a single 10-bp Ets motif (core: GGAA) in the proximal segment, whereas three known inactive ovine genes contain a mutated core motif (TGAA). Cotransfection of a promoter-(؊126 to ؉50) luciferase reporter construct from an active gene (bovineIFN-1; boIFNT1) and an Ets-2 expression plasmid in human JAr cells provided up to a 30-fold increase in reporter expression, whereas promoters from inactive genes were not transactivated. GABP␣ alone was ineffective and had only a Ϸ2-fold positive effect when coexpressed with its partner GABP␤. Other Ets-related transcription factors, which were not detected in the genetic screen, also provided a range of lesser transactivation effects. Coexpression of Ets-2 and activated Ras failed to transactivate the IFNT promoter greater than Ets-2 alone in JAr cells. The presence of Ets-2 in nuclei of embryonic trophectoderm was confirmed immunocytochemically. Together, these data suggest that Ets-2 plays a role in the transient expression of the nonvirally inducible IFNT genes.

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A role for interferons in early pregnancy

Roberts

1991

BioEssays

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In order to survive, the developing conceptus must interrupt the normal ovarian cycle of the mother and extend the production of progesterone by the corpus luteum. An unusual Type 1 interferon (IFN), related structurally to the IFN-alpha molecule and produced in massive amounts for only a few days by the first epithelium (trophectoderm) of the preimplantation conceptus, has been implicated as the antiluteolytic agent in sheep and cattle. IFN-alpha therapy during this critical period can also improve pregnancy success in sheep. It remains unclear, however, whether the trophoblast IFN have specialized biological properties or whether they are unique merely in the timing, magnitude and site of their expression.

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Sequence variability among ovine trophoblast interferon cDNA

Cited by 29 publications

References 4 publications

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

Control of interferon-τ gene expression by Ets-2

A role for interferons in early pregnancy

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