2005
DOI: 10.1016/j.ibmb.2004.10.008
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Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring

Abstract: Toxin-binding proteins of insect midgut epithelial cells are associated with insect resistance to Bacillus thuringiensis (Bt) Cry toxins. A 5378 nt cDNA encoding a 1717 amino acid putative midgut cadherin-like glycoprotein and candidate Cry1Ab toxin-binding protein was characterized from Ostrinia nubilalis.

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Cited by 19 publications
(28 citation statements)
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“…Single-locus STS markers were shown by pedigree analysis for O. nubilalis cadherin (Coates et al 2005a), ommochrome binding proteins (obp1 and obp2) (Coates et al 2005b), brainiac/bre5 (Coates et al 2007), and aminopeptidase 1 (APN1) (Coates et al 2008). Additional single locus markers were from OnZ1 and OnW1 (Coates and Hellmich 2003) and kettin (ket), triosphosphate isomerase (tpi), and lactose dehydrogenase (ldh) (Dopman et al 2005;Malausa et al 2007).…”
Section: Marker Development and Onb1 Pcr Screeningmentioning
confidence: 99%
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“…Single-locus STS markers were shown by pedigree analysis for O. nubilalis cadherin (Coates et al 2005a), ommochrome binding proteins (obp1 and obp2) (Coates et al 2005b), brainiac/bre5 (Coates et al 2007), and aminopeptidase 1 (APN1) (Coates et al 2008). Additional single locus markers were from OnZ1 and OnW1 (Coates and Hellmich 2003) and kettin (ket), triosphosphate isomerase (tpi), and lactose dehydrogenase (ldh) (Dopman et al 2005;Malausa et al 2007).…”
Section: Marker Development and Onb1 Pcr Screeningmentioning
confidence: 99%
“…Final PCR amplification was 35 cycles of 96 8C for 20 s, 50 8C for 30 s, and 72 8C for 1 min. PCR products for O. nubilalis OnZ1 and OnW1,cadherin,obp1,obp2, and bre5 took place according to Coates and Hellmich (2003) and Coates et al (2005aCoates et al ( , 2005bCoates et al ( ), 2007, respectively. Entire PCR product volumes were separated on 1.5% agarose gels and positive BAC SPs or RPs was identified by presence-absence of the gene fragment compared with positive control DNA.…”
Section: Marker Development and Onb1 Pcr Screeningmentioning
confidence: 99%
“…Genomic DNA remaining after AFLP template preparation was diluted to *10 ng/ll with deionize nucleasefree water. SNP markers previously developed for the candidate Bt-resistance genes brainiac (brn), cadherin (cad), and aminopeptidase N (apn1 and apn3) were used to genotype initial parents, FQ4 and FQ5 backcross parents, and subsequent backcross progeny reared on untreated control diet, using PCR-RFLP methods described by Coates et al (2005Coates et al ( , 2007Coates et al ( , 2008a. Additionally, locusspecific markers for a larval midgut-expressed membranebound alkaline phosphatase (malp) were PCR-amplified with mALP-F3 (5 0 -CCA GCG CAA CGG CCA GAC-3 0 ) and mALP-R3d1 (5 0 -GAT TT GA GC GT CC AG GCA ATA-3 0 ) using conditions described by Coates (personal comm.).…”
Section: An Integrated O Nubilalis Aflp-and Snp-based Linkage Mapmentioning
confidence: 99%
“…Compared to candidate gene approaches that have investigated the inheritance of Bt toxin-resistance traits (Coates et al 2005(Coates et al , 2007(Coates et al , 2008a, QTL analysis detect the co-segregation of anonymous markers. Candidate gene studies are limited in scope due to a focus upon one to few loci, and assume that similar traits evolve by analogous biochemical mechanisms.…”
Section: Mapping Of the Larval O Nubilalis Cry1f Resistance Traitmentioning
confidence: 99%
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