Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959–2013

Mosiej, Ewa; Zawadka, Monika; Krysztopa-Grzybowska, Katarzyna; Polak, Maciej; Augustynowicz, Ewa; Piekarska, Katarzyna; Lutyńska, Anna

doi:10.1007/s10096-014-2216-6

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…It has been observed previously that there is little geographical separation of B. pertussis population [15,29]. Overall, ptxP3 B. pertussis circulates in countries where ACV has been in use while WCV countries have been dominated by ptxP1 strains [52]. The replacement of WCV in China in 2012 occurred a decade later than developed countries such as the US and Australia [6].…”

Section: Discussionmentioning

confidence: 99%

Genomic epidemiology of erythromycin-resistant Bordetella pertussis in China

Wang

Luan

et al. 2019

Emerging Microbes & Infections

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Macrolides such as erythromycin are the empirical treatment of Bordetella pertussis infections. China has experienced an increase in erythromycin-resistant B. pertussis isolates since they were first reported in 2013. Here, we undertook a genomic study on Chinese B. pertussis isolates from 2012 to 2015 to elucidate the origins and phylogenetic relationships of erythromycin-resistant B. pertussis isolates in China. A total of 167 Chinese B. pertussis isolates were used for antibiotic sensitivity testing and multiple locus variable-number tandem repeat (VNTR) analysis (MLVA). All except four isolates were erythromycin-resistant and of the four erythromycin-sensitive isolates, three were non-ptxP1 . MLVA types (MT), MT55, MT104 and MT195 were the predominant types. Fifty of those isolates were used for whole genome sequencing and phylogenetic analysis. Genome sequencing and phylogenetic analysis revealed three independent erythromycin-resistant lineages and all resistant isolates carried a mutation in the 23S rRNA gene. A novel fhaB3 allele was found uniquely in Chinese ptxP1 isolates and these Chinese ptxP1-ptxA1-fhaB3 had a 5-fold higher mutation rate than the global ptxP1-ptxA1 B. pertussis population. Our results suggest that the evolution of Chinese B. pertussis is likely to be driven by selection pressure from both vaccination and antibiotics. The emergence of the new non-vaccine fhaB3 allele in Chinese B. pertussis population may be a result of selection from vaccination, whereas the expansion of ptxP1-fhaB3 lineages was most likely to be the result of selection pressure from antibiotics. Further monitoring of B. pertussis in China is required to better understand the evolution of the pathogen.

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Section: Discussionmentioning

confidence: 99%

Genomic epidemiology of erythromycin-resistant Bordetella pertussis in China

Wang

Luan

et al. 2019

Emerging Microbes & Infections

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“…The expansion of ptxP3 strains has been suggested to be associated with the replacement of WCV by ACV although this hypothesis has been controversial [42]. In Poland where WCV has also been in use for decades, in 2000–2013, ptxP1 isolates predominated with 77.5% [43]. However, in a more recent study, 61.5% of the isolates during 2010–2016 were found to be ptxP3 and were attributed to the increasing use of ACV since 2013 for primary immunization [43–45].…”

Section: Discussionmentioning

confidence: 99%

“…In Poland where WCV has also been in use for decades, in 2000–2013, ptxP1 isolates predominated with 77.5% [43]. However, in a more recent study, 61.5% of the isolates during 2010–2016 were found to be ptxP3 and were attributed to the increasing use of ACV since 2013 for primary immunization [43–45]. The near complete replacement of the B. pertussis population in Iran by ptxP3 strains where only WCV has been used further suggests that the expansion of ptxP3 strains was not driven by ACV selection alone.…”

Section: Discussionmentioning

confidence: 99%

Genomic epidemiology of Iranian Bordetella pertussis : 50 years after the implementation of whole cell vaccine

Safarchi

Octavia

Nikbin

et al. 2019

Emerging Microbes & Infections

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Pertussis caused by Bordetella pertussis, remains a public health problem worldwide, despite high vaccine coverage in infants and children in many countries. Iran has been using whole cell vaccine for the last 50 years with more than 95% vaccination rate since 1988 and has experienced pertussis resurgence in recent years. Here, we sequenced 55 B. pertussis isolates mostly collected from three provinces with the highest number of pertussis cases in Iran, including Tehran, Mazandaran, and Eastern-Azarbayjan from the period of 2008-2016. Most isolates carried ptxP3/prn2 alleles (42/55, 76%), the same genotype as isolates circulating in acellular vaccine-administrating countries. The second most frequent genotype was ptxP3/prn9 (8/55, 14%). Only three isolates (5%) were ptxP1. Phylogenetic analysis showed that Iranian ptxP3 isolates can be divided into eight clades (Clades 1-8) with no temporal association. Most of the isolates from Tehran grouped together as one distinctive clade (Clade 8) with six unique single nucleotide polymorphisms (SNPs). In addition, the prn9 isolates were grouped together as Clade 5 with 12 clade-supporting SNPs. No pertactin deficient isolates were found among the 55 Iranian isolates. Our findings suggest that there is an ongoing adaptation and evolution of B. pertussis regardless of the types of vaccine used.

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“…Do tej pory opisano 13 alleli genu podjednostki S1 toksyny krztuścowej: ptxA1-ptxA13, kodujących 6 wariantów białka [65,70]. Dla genu ptxC, kodującego podjednostkę S3 toksyny krztuścowej, dotychczas opisano trzy allele -ptxC1, ptxC2, ptxC3, kodujące trzy warianty białka [70]. Gen kodujący pertaktynę charakteryzuje się wysokim polimorfizmem.…”

Section: Bordetella Pertussisunclassified