Sequences and coding strategies of the S RNAs of Toscana and Rift Valley fever viruses compared to those of Punta Toro, Sicilian sandfly fever, and Uukuniemi viruses

Giorgi, Colomba; Accardi, Luisa; Nicoletti, Loredana; Grò, Maria Cristina; Takehara, Kazuhiko; Hilditch, C. M.; Morikawa, Shigeru; Bishop, David H. L.

doi:10.1016/0042-6822(91)90087-r

Cited by 183 publications

(136 citation statements)

References 28 publications

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“…This ORF encodes a protein of expected size 331-5K which, based on sequence comparisons with the Bunyamwera virus L protein and influenza virus PB1, is believed to be the viral transcriptase. The S RNA segment of TSWV has an ambisense gene arrangement, as is also found in the S RNAs of phlebo-and uukuviruses, two other genera within the Bunyaviridae (Ihara et al, 1984;Marriot et al, 1989;Simons et al, 1990;Giorgi et al, 1991). The gene encoding the N protein is located on the vc strand, while a second gene, encoding a non-structural protein (NSs) of 52.4K, is found on the viral (v) strand (de Haan et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants

Kormelink¹,

Haan²,

Peters³

et al. 1992

Journal of General Virology

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The synthesis of viral RNA species in tomato spotted wilt virus-infected Nicotiana rustica plants was followed in terms of time and relative abundance. Systemic symptoms were visible after 4 days postinoculation (p.i.), but viral (v) and viral-complementary (vc) strands of all three genomic RNA segments [large (L) RNA, medium (M) RNA and small (S) RNA] were detected from 2 days p.i. In addition, two subgenomic mRNAs, derived from S RNA, were detected. For the L RNA segment no subgenomic mRNAs were detected, suggesting that this segment is expressed via the synthesis of a genome-sized vc mRNA. A possible M-specific subgenomic mRNA was detected, showing a similar time course of appearance as the subgenomic mRNAs derived from the S RNA segment. Analysis of cytoplasmic RNA fractions revealed that both v and vc strands of all three genomic segments associate with the nucleocapsid protein into nucleocapsid structures, the vcRNA species being present in lower amounts. Intact, enveloped virus particles contained only the v strand of the L RNA segment and, surprisingly, both v and vc strands of the M and S RNA segment, though in different ratios.

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Section: Introductionmentioning

confidence: 99%

Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants

Kormelink¹,

Haan²,

Peters³

et al. 1992

Journal of General Virology

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“…These viruses contain a negative-sense, single-stranded RNA genome, consisting of three segments, designated large (L), medium (M) and small (S) (Elliott, 1990). The S segment exhibits an ambisense coding strategy and encodes the N and NSs proteins from mRNAs of opposite polarities (Giorgi et al, 1991). The M segment of viruses in the phlebotomus fever group, but not viruses in the Uukuniemi group, has a pre-glycoprotein coding region that encodes a non-structural protein (NSm) (Elliott et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses

Liu

Tesh

Rosa

et al. 2003

Journal of General Virology

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Viruses in the Phlebovirus genus of the family Bunyaviridae cause clinical syndromes ranging from a short, self-limiting febrile illness to fatal haemorrhagic fever. The genus currently consists of 68 antigenically distinct virus serotypes, most of which have not been genetically characterized. RT-PCR with four 'cocktail' primers was performed to amplify a region of the M segment of the genome of 24 phleboviruses included in the sandfly fever Naples, sandfly fever Sicilian and Punta Toro serocomplexes. Partial M segment sequences were successfully obtained and phylogenetic analysis was performed. The three resultant genotypic lineages were consistent with serological data. The sequence divergences were 27?6 % (nucleotide) and 25?7 % (amino acid) within the Sicilian serocomplex, 33?7 % (nucleotide) and 34?4 % (amino acid) within the Naples serocomplex and 35?6 % (nucleotide) and 37?5 % (amino acid) within the Punta Toro serocomplex. Overall, the diversities among viruses of Sicilian, Naples and Punta Toro serocomplexes were 48?2 % and 57?6 % at the nucleotide and amino acid levels, respectively. This high genetic divergence may explain the difficulties in designing a consensus primer pair for the amplification of all the phleboviruses using RT-PCR. It also suggests that infection with one genotype may not completely immunize against infection with all other genotypes in a given serocomplex. These findings have implications for potential vaccine development and may help explain clinical reports of multiple episodes of sandfly fever in the same individual.

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“…The termini of the UTRs are conserved and form panhandle structures [26-28] which give the RNPs a circular appearance when observed by electron microscopy [29-31]. For RVFV, the L and M segments are of negative polarity while the S segment utilizes an ambisense strategy to code for both N and NSs [32]. The coding capacity of the genome is depicted in Fig.…”

Section: The Family Bunyaviridaementioning

confidence: 99%

Molecular Biology of Rift Valley Fever Virus

Bouloy¹

2010

TOVJ

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Rift Valley fever virus (RVFV) causes large outbreaks of acute febrile and often fatal illness among humans and domesticated animals in sub-saharan Africa and the Arabian peninsula. RVFV is a member of the family Bunyaviridae, genus Phlebovirus. Like all members of this large virus family, it contains a three-segmented genome of negative/ambisense strand RNA, packaged into viral nucleocapsid protein, and enveloped by a lipid bilayer containing two viral glycoproteins. During the past years, there was an increased interest in RVFV epidemiology, molecular biology, and virulence mechanisms. Here, we will try to provide an overview over the basic features of this significant pathogen, and review the latest developments in this highly active research field.

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Sequences and coding strategies of the S RNAs of Toscana and Rift Valley fever viruses compared to those of Punta Toro, Sicilian sandfly fever, and Uukuniemi viruses

Cited by 183 publications

References 28 publications

Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants

Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants

Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses

Molecular Biology of Rift Valley Fever Virus

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