Sequencing and characterization of complete mitogenome DNA of Rasbora tornieri (Cypriniformes: Cyprinidae: Rasbora) and its evolutionary significance

Abstract

The yellowtail rasbora (Rasbora tornieri) is a miniature ray-finned fish categorized under the genus Rasbora in the family of Cyprinidae. In this study, a complete mitogenome sequence of R. tornieri was sequenced using four primers targeting two halves of the mitogenome with overlapping flanking regions. The size of mitogenome was 16,573 bp, housing 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes and a putative control region. Identical gene organization was detected between this species and other members of Rasbora genus. The heavy strand encompassed 28 genes while the light strand accommodated the other nine genes. Most protein-coding genes execute ATG as start codon, excluding COI and ND3 genes, which utilized GTG instead. The central conserved sequence blocks (CSB-E, CSB-F and CSB-D), variable sequence blocks (CSB-1, CSB-3 and CSB-2) as well as the terminal associated sequence (TAS) were conserved within the control region. The maximum likelihood phylogenetic family tree revealed the divergence of R. tornieri from the basal region of the Rasbora clade, where its evolutionary relationships with other Rasbora members are poorly resolved as indicated by the low bootstrap values. This work acts as window for further population genetics and molecular evolution studies of Rasbora genus in future.

Identification of optimal codons and analysis of phylogenetic relationship in Osteochilus salsburyi (Teleostei: Cypriniformes) based on complete mitogenome

“…Of there, 14 tRNAs were encoded on the H-strand, while the remaining eight tRNAs were coded on the L-strand. The coding pattern was commonly observed in almost all Cyprinidae mitogenomes (Wang et al, 2008;Lim et al, 2019;Chung et al, 2020). The primitive arrangement of these tRNA genes in the five Osteochilus species was the same as in the common vertebrate arrangement (Boore, 1999).…”

Comprehensive Analysis of Phylogenetic Relationship and Optimal Codons in Mitochondrial Genomes of the Genus Pseudogastromyzon

“…In the 11 Pseudogastromyzon mitogenomes, the arrangement of tRNAs on both the heavy and light strands remained consistent, encompassing 14 tRNAs encoded on the H-strand and 8 tRNAs on the L-strand. This distribution pattern is a prevalent feature in many fish mitogenomes, akin to Rasbora tornieri [43], Osteochilus salsburyi [10], and Rhynchocypris oxycephalus [44]. Among the 22 tRNAs, the arrangement order of these genes was the same as that of vertebrate [7], and 21 tRNAs could be folded into the typical clover-leaf secondary structures, except for the trnS1 gene lacking dihydrouracil arms.…”

A Review on the Emerging Asian Aquaculture Fish, the Malaysian Mahseer (Tor tambroides): Current Status and the Way Forward

“…The mitogenome sequencing is one pivotal gateway to the comprehensive elucidation of the underlying molecular evolution and population genetics of an organism (Chen et al 2016;Chung et al 2020a;Lim et al 2019a). The mitogenome tells a story about how the same fish species may look different phenotypically when segregated geographically, as part of their adaptation strategy to survive in harsh environment (Chung et al 2020b;Lim et al 2018a). This maternally inherited organellar genome has been proven powerful in improving the phylogenetic resolution of some fishes with high cryptic diversity, especially those from the Cyprinidae family, when coupled with both morphometric and meristic data (Aminan et al 2020).…”