Sequential Development of Cysteine Proteinase Activities and Gene Expression during Somatic Embryogenesis in Carrot

Mitsuhashi, Wataru; Yamashita, Taroh; Toyomasu, Tomonobu; Kashiwagi, Yoshiko; Konnai, Tomoko

doi:10.1271/bbb.68.705

Cited by 13 publications

(45 citation statements)

References 44 publications

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“…CP gene identification has occurred in several plant sources, such as Mesembryanthemum crystallinum (Forsthoefel et al, 1998), Daucus carota (Mitsuhashi et al, 2004), and C. candamarcensis (Pereira et al, 2001). Over-expression of CP genes from plant sources, like C. papaya (Taylor et al, 1999), Ipomoea batatas (Chen et al, 2009;2010), Nicotiana tabacum (Beyene et al, 2006;Zhang et al, 2009), and C. candamarcensis (Corrêa et al, 2011), has also been reported and shown to be encouraging examples of the aims presented in this study.…”

Section: Introductionsupporting

confidence: 53%

Isolation of cDNA from Jacaratia mexicana encoding a mexicain-like cysteine protease gene

Ramos-Martinez

Herrera-Ramírez

Badillo-Corona

et al. 2012

Gene

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Section: Introductionsupporting

confidence: 53%

Isolation of cDNA from Jacaratia mexicana encoding a mexicain-like cysteine protease gene

Ramos-Martinez

Herrera-Ramírez

Badillo-Corona

et al. 2012

Gene

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“…Cysteine and aspartic proteases have been implicated in protein maturation, execution of PCD, germination, and tissue remodeling (Beers et al 2004). The induction of cysteine proteases correlates with somatic embryogenesis induction (Thibaud‐Nissen et al 2003, Mitsuhashi et al 2004), and several proteases are induced during zygotic embryogenesis (Dong et al 2004, Sreenivasulu et al 2004). During androgenesis induction, several studies have reported that one of the main changes brought by stress is represented by an overall decrease in total protein in the microspores (Kyo and Harada 1990, Garrido et al 1993, Ríhová et al 1996).…”

Section: Resultsmentioning

confidence: 99%

cDNA array analysis of stress‐induced gene expression in barley androgenesis

Maraschin¹,

Caspers

Potokina

et al. 2006

Physiologia Plantarum

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Different aspects of androgenesis induction have been studied in detail, but little is known about the molecular mechanisms associated with this developmental switch. We have employed macroarrays containing 1421 expressed sequence tags covering the early stages of barley zygotic embryogenesis to compare the gene expression profiles of stress-induced androgenic microspores with those of uninucleate microspores as they progressed into binucleate stage during pollen development. Principal component analysis defined distinct sets of gene expression profiles that were associated with androgenesis induction and pollen development. During pollen development, uninucleate microspores were characterized by the expression of cell division-related genes and transcripts involved in lipid biosynthesis. Progress into binucleate stage resulted in the significant increase in the level of transcripts associated with starch biosynthesis and energy production. These transcripts were downregulated in androgenic microspores. These results indicate that stress blocks the expression of pollen-related genes. The induction of androgenesis by stress was marked by the upregulation of transcripts involved in sugar and starch hydrolysis, proteolysis, stress response, inhibition of programmed cell death, and signaling. Further expression analysis revealed that the induction of genes encoding alcohol dehydrogenase 3, metalloprotease FtsH, cysteine protease 1 precursor, phytepsin precursor (aspartic protease), and a 26S proteasome regulatory subunit was associated with the androgenic potential of microspores, whereas the induction of transcripts involved in signaling and cytoprotection was associated with stress responses. Taken together, these expression profiles represent 'bio-markers' associated with the androgenic switch in microspores, providing a substantial contribution toward understanding the molecular events underlying stress-induced androgenesis.

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“…In ARP and EAR, a predicted cysteine proteinase RD21a-like (spot 0219) was up-regulated, indicating that cysteine proteinase also has a role in rooting. Several studies indicated that cysteine proteinase activity is involved in carrot somatic embryo development (Maës et al 1997;Mitsuhashi et al 2004). One enzyme with the hydrolytic activity, predicted 26S protease regulatory subunit 6B homolog (spot 4515) was up-regulated in ARP.…”

Section: Functional Analysis Of the Identified Proteinsmentioning

confidence: 99%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

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Key message Comparative proteome analysis revealed the proteins that are differentially expressed during the processes of tetraploid black locust (Robinia pseudoacacia L.) adventitious root formation, which are involved in carbohydrate binding, energy and metabolism, and stress response. Abstract The tetraploid black locust (Robinia pseudoacacia L.) is an important fast-growing tree used in afforestation and is also a protein-rich feed for poultry. Currently, the primary approach for tetraploid black locust reproduction is through cutting propagation. To identify the specific proteins in the rooting process, including the formation of adventitious root primordium (ARP) and the elongation of adventitious root (EAR), a comparative proteomic analysis of the ARP and EAR of tetraploid black locust was performed to identify related proteins that may be involved in regulating ARP formation and the process of EAR. High-resolution two-dimensional electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric (MS) analysis was used to identify differentially expressed proteins in ARP and EAR. A total of 84 protein spots showed significant expression changes by 2-DE and were successfully identified by MALDI TOF/ TOF MS/MS. Of these, 29 proteins were differentially expressed during the ARP phase, and the remaining 55 proteins did so in the EAR phase. The majority of identified proteins were classified into functional categories, including carbohydrate binding, energy and metabolism, protein degradation/folding and import, cellular cytoskeleton, hormonerelated and oxidation reactions, stress response, cell proliferation, and transcription regulation. Quantitative real-time PCR analysis was performed for selected proteins and showed that not all of the protein expression levels were consistent with the mRNA levels. This study provides the basis for further functional studies of differentially expressed proteins, which will contribute to the understanding of the biochemical processes in adventitious root primordium formation and the elongation of adventitious root. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI Matrix assisted laser desorption ionization TOF Time of flight TOF MS Time of flight mass spectrometry MS Mass spectrometry IntroductionTetraploid black locust (Robinia pseudoacacia L.) (TRP), which can be produced by doubling the chromosomes of a diploid black locust (2n 9 2) artificially, is a leguminous Communicated by R. Hampp. Electronic supplementary materialThe online version of this article (

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Sequential Development of Cysteine Proteinase Activities and Gene Expression during Somatic Embryogenesis in Carrot

Cited by 13 publications

References 44 publications

Isolation of cDNA from Jacaratia mexicana encoding a mexicain-like cysteine protease gene

Isolation of cDNA from Jacaratia mexicana encoding a mexicain-like cysteine protease gene

cDNA array analysis of stress‐induced gene expression in barley androgenesis

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

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