1991
DOI: 10.1016/s0021-9258(20)64329-0
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Sequential processing of epidermal growth factor in early and late endosomes of rat liver

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Cited by 47 publications
(12 citation statements)
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“…Despite the inhibition of inward vesiculation, EGFRs are still delivered to the lysosome in the presence of wortmannin, and EGF degradation is largely unaffected. Although some proteolytic processing of EGF has been reported to occur in prelysosomal compartments (Schaudies et al, 1987;Renfrew and Hubbard, 1991), we have shown previously that MVB-lysosome fusion is required for the degradation of EGF to TCA-soluble products (Futter et al, 1996). Therefore, we conclude that MVB-lysosome fusion is not affected by wortmannin treatment.…”
Section: Wortmannin Treatment Inhibits Inward Vesiculation Within Mvbs But Not Egfr Delivery To the Lysosomementioning
confidence: 51%
“…Despite the inhibition of inward vesiculation, EGFRs are still delivered to the lysosome in the presence of wortmannin, and EGF degradation is largely unaffected. Although some proteolytic processing of EGF has been reported to occur in prelysosomal compartments (Schaudies et al, 1987;Renfrew and Hubbard, 1991), we have shown previously that MVB-lysosome fusion is required for the degradation of EGF to TCA-soluble products (Futter et al, 1996). Therefore, we conclude that MVB-lysosome fusion is not affected by wortmannin treatment.…”
Section: Wortmannin Treatment Inhibits Inward Vesiculation Within Mvbs But Not Egfr Delivery To the Lysosomementioning
confidence: 51%
“…These complexes are delivered intact to this compartment since their degradation can be inhibited >80% by DAB cross-linking. In other cell types, most notably the hepatocyte, it has been suggested that partial proteolytic processing of internalized EGF may occur at an early stage in the endocytic pathway (Renfrew and Hubbard, 1991). We cannot rule out the possibility that partial processing events occur before fusion of the MVB with the lysosome, but it is clear that the full degradation of EGF to TCA-soluble products, as in the hepatocyte (Renfrew and Hubbard, 1991), requires delivery to the lysosome.…”
Section: Docking and Fusion Of Egfr ÷ Mvb With Lysosomesmentioning
confidence: 88%
“…In other cell types, most notably the hepatocyte, it has been suggested that partial proteolytic processing of internalized EGF may occur at an early stage in the endocytic pathway (Renfrew and Hubbard, 1991). We cannot rule out the possibility that partial processing events occur before fusion of the MVB with the lysosome, but it is clear that the full degradation of EGF to TCA-soluble products, as in the hepatocyte (Renfrew and Hubbard, 1991), requires delivery to the lysosome. That the HRP only labels a subset of lysosomes is shown by the inhibition of only 55% of cell n'acetylglucosaminidase activity and by the light and electron micoscopical observations that show that there are many LAMP-1 positive lysosomes that contain neither EGF nor HRP.…”
Section: Docking and Fusion Of Egfr ÷ Mvb With Lysosomesmentioning
confidence: 88%
“…Thus, one fate of the internal vesicles is the degradation of their components. A well-characterized example is the epidermal growth factor receptor, which is endocytosed and accumulates at the MVB's internal membrane vesicles only after binding of a cognate ligand (Felder et al, 1990;Renfrew and Hubbard, 1991). A second defined role for MVBs stems from their ability to fuse with the plasma membrane.…”
Section: Introductionmentioning
confidence: 99%