Sequential selection and efficacy of antagonistic rhizobacteria for controlling Phytophthora blight of pepper

Kim, Hye Sook; Sang, Mee Kyung; Jeun, Yong Chull; Hwang, Byung Kook; Kim, Ki Deok

doi:10.1016/j.cropro.2007.07.013

Cited by 47 publications

(35 citation statements)

References 32 publications

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“…La producción de chile dulce (Capsicum annuum L.) tanto en Costa Rica como en otras partes del mundo, está limitada por la enfermedad conocida como pudrición basal del tallo o maya, causada por el oomycete Phytophthora capsici Leonian (Solís, 1989;Corrales et al, 1990;Kim et al, 2008). La enfermedad se presenta en áreas tropicales y subtropicales e infecta tanto raíces, tallos, hojas y frutos (Kim et al, 1997), siendo una de las enfermedades más destructivas a nivel mundial (Ristaino y Johnston, 1999;Hausbeck y Lamour, 2004).…”

Section: Introductionunclassified

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Pudrición basal causada por Phytophthora capcisi en plantas de chile tratadas con vermicompost

et al. 2014

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El objetivo de este trabajo fue evaluar a nivel de invernadero el efecto de vermicompost sobre la incidencia y severidad de Phytophthora capcisi en plantas de chile (Capsicum annuum). Esta investigación se realizó entre febrero y marzo del 2012 en el Centro de Investigaciones Agronómicas, Sabanilla, San José, Costa Rica. Se utilizó un diseño completamente al azar con cuatro repeticiones y ocho plantas por unidad experimental en un arreglo factorial con los factores de dosis (0, 25% y 50% v/v vermicompost:suelo) e inoculación (0 y 500 zoosporas por gramo de suelo). Se trasplantaron plántulas de 35 días, que fueron inoculadas dos semanas después del trasplante. La aplicación de vermicompost provocó un aumento significativo del peso fresco foliar y del peso seco foliar y radical; a mayor dosis, mayor fue el incremento. Las plantas de todos los tratamientos que incluyeron inoculación con P. capsici presentaron síntomas de la enfermedad en la raíz. Los valores de incidencia y severidad fueron mayores para el tratamiento con 50% de abono. En este tratamiento se presentaron síntomas de marchitez. La ausencia de diferencias en las variables de peso fresco y seco entre los tratamientos inoculados y sin inocular, a los que se adicionó abono al 25%, sugiere que esta dosis podría compensar el daño causado por el patógeno. Se observó una menor concentración de nutrimentos en los tejidos de las plantas inoculadas lo que indica que el daño causado a la raíz pudo haber afectado la adquisición de nutrimentos.

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Section: Introductionunclassified

“…Debido a la naturaleza persistente del inóculo en el suelo, la estrategia para el control de este patógeno requiere un programa de manejo integral (Kim et al, 2008). Sin embargo, las medidas de control químico y cultural no siempre logran disminuir el inóculo de P. capsici, formado por zoosporas con alta movilidad en el suelo.…”

Section: Introductionunclassified

Pudrición basal causada por Phytophthora capcisi en plantas de chile tratadas con vermicompost

et al. 2014

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“…An oomycete Phytophthora capsici is one of the most destructive soilborne pathogens in pepper and other vegetables such as cucumber, pumpkin, beet, and turnip (Hausbeck and Lamour 2004). Biological control using root‐associated microbes or organic products in agricultural systems has been proposed as an environmental‐friendly disease management strategy (Kim et al. 2008, 2009, 2012; Sang et al.…”

Section: Introductionmentioning

confidence: 99%

The volatile-producing Flavobacterium johnsoniae strain GSE09 shows biocontrol activity against Phytophthora capsici in pepper

2012

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Aims: Previously, we selected a bacterial strain (GSE09) antagonistic to Phytophthora capsici on pepper, which produced a volatile compound (2,4‐di‐tert‐butylphenol), inhibiting the pathogen. In this study, we identified strain GSE09 and characterized some of the biological traits of this strain in relation to its antagonistic properties against P. capsici. In addition, we examined bacterial colonization on the root surface or in rhizosphere soil and the effect of various concentrations of the volatile compound and strain GSE09 on pathogen development and radicle infection as well as radicle growth. Methods and Results: Strain GSE09 was identified as Flavobacterium johnsoniae, which forms biofilms and produces indolic compounds and biosurfactant but not hydrogen cyanide (HCN) with little or low levels of antifungal activity and swimming and swarming activities. Fl. johnsoniae GSE09 effectively colonized on pepper root, rhizosphere, and bulk (pot) soil, which reduced the pathogen colonization in the roots and disease severity in the plants. Various concentrations of 2,4‐di‐tert‐butylphenol or strain GSE09 inhibited pathogen development (mycelial growth, sporulation, and zoospore germination) in I‐plate (a plastic plate containing a center partition). In addition, germinated seeds treated with the compound (1–100 μg ml−1) or the strain (102–1010 cells ml−1) significantly reduced radicle infection by P. capsici without radicle growth inhibition. Conclusions: These results indicate that colonization of pepper root and rhizosphere by the Fl. johnsoniae strain GSE09, which can form biofilms and produce indolic compounds, biosurfactant, and 2,4‐di‐tert‐butylphenol, might provide effective biocontrol activity against P. capsici. Significance and Impact of the Study: To our knowledge, this is the first study demonstrating that the Fl. johnsoniae strain GSE09, as a potential biocontrol agent, can effectively protect pepper plants against P. capsici infection by colonizing the roots.

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“…Kim et al (1989), Kim et al (2008) and Sang et al (2008) reported that it has taken 5−7 days until the symptom appearance of Phytophthora blight of peppers in the field plots when 9~10-week old pepper seedlings were inoculated with zoospore suspension (10 4~1 0 5 zoospores/ml) by soil drenching around individual plants. Considering the favorable conditions for artificial inoculation in their experiments, the average incubation period of 8 ± 3 days as suggested by the infection risk model would be a plausible time period between the primary infection and the first occurrence of disease symptoms for Phytophthora blight epidemics occurring under natural conditions in the field.…”

Section: Discussionmentioning

confidence: 99%

A Forecast Model for the First Occurrence of Phytophthora Blight on Chili Pepper after Overwintering

Seok¹,

Kang²,

Park³

2012

The Plant Pathology Journal

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An infection risk model for Phytophthora blight on chili pepper was developed to estimate the first date of disease occurrence in the field. The model consisted of three parts including estimation of zoosporangium formation, soil water content, and amount of active inoculum in soil. Daily weather data on air temperature, relative humidity and rainfall, and the soil texture data of local areas were used to estimate infection risk level that was quantified as the accumulated amount of active inoculum during the prior three days. Based on the analysis on 190 sets of weather and disease data, it was found that the threshold infection risk of 224 could be an appropriate criterion for determining the primary infection date. The 95% confidence interval for the difference between the estimated date of primary infection and the observed date of first disease occurrence was 8 ± 3 days. In the model validation tests, the observed dates of first disease occurrence were within the 95% confidence intervals of the estimated dates in the five out of six cases. The sensitivity analyses suggested that the model was more responsive to temperature and soil texture than relative humidity, rainfall, and transplanting date. The infection risk model could be implemented in practice to control Phytophthora blight in chili pepper fields.

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Sequential selection and efficacy of antagonistic rhizobacteria for controlling Phytophthora blight of pepper

Cited by 47 publications

References 32 publications

Pudrición basal causada por Phytophthora capcisi en plantas de chile tratadas con vermicompost

Pudrición basal causada por Phytophthora capcisi en plantas de chile tratadas con vermicompost

The volatile-producing Flavobacterium johnsoniae strain GSE09 shows biocontrol activity against Phytophthora capsici in pepper

A Forecast Model for the First Occurrence of Phytophthora Blight on Chili Pepper after Overwintering

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