Seroconversion to virus-specific pre-early nuclear antigens in infants with primary cytomegalovirus infection

Chiba, S; Motokawa, T; Tamura, Tadashi; Hanazono, Hisaaki; Kamada, Makoto; Chiba, Yasuo; Nakao, Toshiko

doi:10.1128/iai.30.1.135-139.1980

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…In human sera anti-CMV antibodies are mainly directed against CMV intracellular (nuclear and cytoplasmic) antigens (Fig. 1D), and the sera may contain only small amounts of antibodies with specificity for CMV-MA (2,5,13,15,16,26). Using human sera, we found that even mild fixation of CMV-infected cells prior to immunofluorescence causes considerable damage to the cell membranes, thereby making CMV intracellular (including nuclear) antigens accessible to their corresponding antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…For these studies, a convenient method is required that allows the analysis of multiple samples for the presence of antibodies with specificity for CMV-MA. Indirect immunofluorescence and immunoperoxidase staining of viable (1,3,5,13,16,17,22,23,26) or air-dried (8) CMV-infected cells have been used to measure antibody binding to CMV-MA. However, the specificity of the membrane staining reaction was not always reported (1,5,8,16,17).…”

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confidence: 99%

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Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens

Middeldorp¹,

Jongsma²

1986

J Clin Microbiol

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This paper describes an improved method for the in vitro detection of antibodies specifically directed against human cytomegalovirus (CMV)-induced membrane antigens present on the surface of CMV-infected fibroblasts (CMV-MA). Viable cells were found to be essential for specific visualization of CMV-MA staining. The addition of divalent cations (2.6 mM Ca2' and 2.2 mM Mg2+) and glucose (180 mM) to the incubation and washing buffers improved the viability and morphology of the cells and increased the cell yield at the end of the assay. Clustering of antigen-antibody complexes on the surface of viable CMV-infected cells was prevented by low-temperature incubation (0 to 4°C) rather than by the addition of agents which act on the metabolism of the cell. No interaction with the CMV-induced Fc receptor was observed at 0°C with either human sera or murine monoclonal antibodies. The specificity of the CMV-MA reaction was confirmed by using monoclonal antibodies to CMV nuclear, cytoplasmic, and membrane-associated antigens. Furthermore, a microplate modification of the membrane fluorescence test is described which is suitable for multisample screening purposes. This method can be applied to the determination of anti-CMV-MA antibody titers in human sera and to the screening of hybridoma supernatants for the presence of antibodies with specificity for CMV-MA.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens

Middeldorp¹,

Jongsma²

1986

J Clin Microbiol

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“…Antibodies against CMV-MA have been detected in sera from patients recovering from acute CMV infections (2,4,8,9,20). In contrast to neutralizing antibodies (10,14), CMV-MA antibodies are detectable only temporarily early after the onset of disease symptoms (2,8,20) and may help to limit viral replication by means of their capacity to mediate the killing of CMV-infected cells (2, 8; J. M. Middeldorp, J. Jongsma, and T. H. The, unpublished data).…”

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confidence: 99%

Cytomegalovirus early and late membrane antigens detected by antibodies in human convalescent sera

Middeldorp¹,

Jongsma²

1985

J Virol

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Using indirect immunofluorescence and a panel of human convalescent-phase sera, we identified cytomegalovirus (CMV) early and late membrane antigens (CMV-EMA and CMV-LMA, respectively) as separate entities on the surfaces of viable CMV-infected fibroblasts starting at 6 to 12 and 36 to 48 h postinoculation, respectively. For expression of CMV-EMA and CMV-LMA, infectious virus and active protein synthesis were required, whereas the expression of CMV-LMA, in addition, required viral DNA synthesis. Our data suggest that CMV-EMA and CMV-LMA form an individual set of CMV antigens that are different from intracellular CMV antigens and possibly (partly) different from the-viral envelope.

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Antibody response against early antigens in Herpesviridae infections

1985

Eur J Epidemiol

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For the serological diagnosis of Herpesvirus infection, increasing use is made of the determination of the antibodies against virus-specific early antigens. The presence of serum antibody to early antigens is a widely accepted marker of acute Epstein-Barr virus infection or Varicella-Zoster virus infection. Controversial data are present in the literature about the significance of Cytomegalovirus early antigens antibody response. This is discussed in view of the possibility that controversial results could be due to different methods used to produce early antigens by different authors.

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Seroconversion to virus-specific pre-early nuclear antigens in infants with primary cytomegalovirus infection

Cited by 8 publications

References 9 publications

Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens

Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens

Cytomegalovirus early and late membrane antigens detected by antibodies in human convalescent sera

Antibody response against early antigens in Herpesviridae infections

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