Serodiagnostic Aids and Management Practice for Feline Retrovirus and Coronavirus Infections

Barlough, Jeffrey E.

doi:10.1016/s0195-5616(84)50101-6

Cited by 16 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous attempts have been made to develop a suitable vaccine against FIP. Unfortunately most have failed, and a number have actively enhanced disease (Woods and Pedersen, 1979;Pedersen and Black, 1983;Barlough, 1984;Pedersen et al, 1984;Pedersen and Floyd, 1985;Barlough and Stoddart, 1990;Gerber et al, 1990;Scott et al, 1992Scott et al, , 1995a. When enhancement occurs the earlier onset of clinical signs and higher mortality results from the presence of antibody to FCoV prior to virulent challenge (Weiss and Scott, 1981a, c;Pedersen, 1987).…”

Section: Discussionmentioning

confidence: 99%

Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)

Gunn-Moore

Caney

Gruffydd-Jones

et al. 1998

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Two recombinant FIPV spike proteins were assessed for their immunogenic properties in 8-week-old kittens, which were then challenged intranasally with FIPV 79-1146. Humoral responses were assessed by ELISA and serum neutralisation test. Changes in PBMC cytokine mRNA levels were detected by a reverse transcription, semiquantitative polymerase chain reaction assay (RT-sqPCR), assessing IL-2, IL-4, IL-6, IL-10, IL-12 and IFNgamma. All of the kittens developed clinical signs typical of FIP, which were confirmed on gross post mortem examination. The recombinant proteins induced little or no specific antibody response prior to challenge, and failed to alter the course of disease compared to controls. One week after virus challenge, the stimulated PBMCs showed small increases in the expression of IL-6 and IFNgamma mRNA, which correlated with a transient pyrexia. After this time expression of IL-6 mRNA remained unaltered but, as FIP developed, mRNA levels of IL-2, IL-4, IL-10, IL-12 and IFNgamma became markedly depressed.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)

Gunn-Moore

Caney

Gruffydd-Jones

et al. 1998

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

show abstract

“…Antibody titers measured in serum are an extensively used diagnostic tool [6,125]. In view of the facts that a large percentage of the healthy cat population is FCoV antibody-positive, that high and rising titers are frequently found in asymptomatic cats, and that most of those cats never develop FIP [126], antibody titers must be interpreted extremely cautiously [7,10,127,128].…”

Section: Measurement Of Antibodiesmentioning

confidence: 99%

Feline infectious peritonitis

Hartmann

2005

Veterinary Clinics of North America: Small Animal Practice

148

251

View full text Add to dashboard Cite

The article discusses feline infectious peritonitis (FIP), an important disease frequently seen in veterinary practice. FIP causes many problems to the veterinarian as it can be difficult to definitively diagnose the disease, as there is no effective treatment, and as prophylactic interventions are not very successful. Although intense research has created a lot of new knowledge about this disease in the last years, there are still many unanswered questions. The objective of this article is to review recent knowledge and to increase understanding of the complex pathogenesis of FIP.

show abstract

“…An indirect fluorescent antibody test was used to detect the presence of coronavirus antibody in the cat sera. 1,10 The sera were diluted 1:25 in phosphate buffered saline (PBS), pH 7.4, and 50 µ1 of each diluted serum was added to a well on a reagent test slide. After incubation in a humid chamber at 37 C for 30 min, the slides were washed 3 times for 5 min in PBS.…”

Section: Serologic Determination Of Antibody To a Coronavirus (Fipv-dmentioning

confidence: 99%

“…11,15 viridae, subfamily Oncovirinae, and was first discovThe family Coronaviridae includes feline infectious ered in Scotland in 1964. 1 Feline leukemia virus inperitonitis virus, feline enteric coronavirus (FECV), fection causes an immunosuppressive syndrome that transmissible gastroenteritis virus (TGEV) of swine, allows an array of secondary infections to occur. 1,2,4,13 It has been reported that 50% of cats with FIPV, chronFrom the Oklahoma Animal Disease Diagnostic Laboratory, Col-ic gingivitis, oral ulcers, or chronic stomatitis are conlege of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078.…”

mentioning

confidence: 99%

A Serologic Survey of Oklahoma Cats for Antibodies to Feline Immunodeficiency Virus, Coronavirus, and Toxoplasma Gondii and for Antigen to Feline Leukemia Virus

Rodgers

Baldwin

1990

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract.A serologic survey was done on 618 cat sera submitted to the Oklahoma Animal Disease Diagnostic Laboratory between July 1, 1987 and June 30, 1988. The samples were collected from clinically normal and sick cats. The sera were tested for the presence of antibodies to feline immunodeficiency virus by a commercial immunoassay, to a coronavirus by an indirect fluorescent antibody test, and to Toxoplasma gondii by a commercial latex agglutination test and for the presence of feline leukemia virus antigen with one of 3 different commercial assay kits. Ten percent of the sera had antibodies to feline immunodeficiency virus, 35% had antibodies to a coronavirus, and 22% had antibodies to Toxoplasma gondii. Feline leukemia virus antigen was detected in 15% of the sera. Thirty-two percent of the sera had evidence of exposure to 2 or more of the agents.

show abstract

Serodiagnostic Aids and Management Practice for Feline Retrovirus and Coronavirus Infections

Cited by 16 publications

References 29 publications

Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)

Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)

Feline infectious peritonitis

A Serologic Survey of Oklahoma Cats for Antibodies to Feline Immunodeficiency Virus, Coronavirus, and Toxoplasma Gondii and for Antigen to Feline Leukemia Virus

Contact Info

Product

Resources

About