Serologic Assay Based on Gliadin-Related Nonapeptides as a Highly Sensitive and Specific Diagnostic Aid in Celiac Disease

Schwertz, Elke; Kahlenberg, Franka; Sack, Ulrich; Richter, Thomas; Stern, Martin; Conrad, Karsten; Zimmer, Klaus–Peter; Mothes, Thomas

doi:10.1373/clinchem.2004.036111

Cited by 116 publications

(113 citation statements)

References 35 publications

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“…To sum up, our study confirms the preliminary data published by other authors, in which it is evident that AGA tests using deamidated gliadin peptides give excellent results in the diagnosis of CD patients (18,19,32). Our very heterogeneous case study demonstrates the particular diagnostic efficiency of the AGAs class IgG tests.…”

Section: Discussionsupporting

confidence: 89%

Diagnostic efficacy of the ELISA test for the detection of deamidated anti‐gliadin peptide antibodies in the diagnosis and monitoring of celiac disease

Tonutti

Visentini

Picierno

et al. 2009

Clinical Laboratory Analysis

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Background and Aim: We evaluated the diagnostic performance of an ELISA test for anti-gliadin IgA and IgG antibodies, which uses synthetic deamidated gliadin peptides (anti-gliadin antibodies, AGAs) as coating; the results were compared with a test that uses extracted gliadin (AGAe). Methods: The study was conducted on the sera of 144 patients suffering from celiac disease (CD), including 20 patients with IgA deficiency and 9 who were following a gluten-free diet (GFD), and 129 controls. Results: In the 115 CD patients (without IgA deficiency), the sensitivity of AGAe IgA and IgG was 32.2 and 60.9%, whereas that of AGAs IgA and IgG was 59.1 and 72.2%. The specificity for AGAe IgA and IgG, and AGAs IgA and IgG was 93.8 and 89.9%, and 96.9% and 99.2%, respectively. Of the 20 patients with CD and IgA deficiency, 7 tested positive for AGAe IgG and 14 for AGAs IgG. The test using deamidated gliadin peptides performed better in terms of sensitivity and specificity than the AGA tests with extracted antigen. Conclusions: The very high specificity of the AGAs IgG test (99.2%) also suggests that patients who test positive with this assay require a thorough followup, even if the anti-tissue transglutaminase antibodies (anti-tTG) and anti-endomysial autoantibodies (EMA) assays are negative.

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Section: Discussionsupporting

confidence: 89%

Diagnostic efficacy of the ELISA test for the detection of deamidated anti‐gliadin peptide antibodies in the diagnosis and monitoring of celiac disease

Tonutti

Visentini

Picierno

et al. 2009

Clinical Laboratory Analysis

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“…In recent years it has been shown that the new anti-DGP ELISA tests using deamidated gliadin peptides as antigen have a high sensitivity and a specificity comparable to those of anti-tTG and EMA and higher than AGA (25,(38)(39)(40).…”

Section: Discussionmentioning

confidence: 99%

“…Schwertz et al (38) demonstrated that an immunoassay based on deamidated synthetic gliadin peptides (DGP) bound to nitrocellulose filters were recognized by sera of CD patients. The recognition of the epitopes containing the sequence QPEQPFP showed high specificity for CD.…”

Section: Introductionmentioning

confidence: 99%

The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?

Brusca

Carroccio²,

Tonutti³

et al. 2012

Clinical Chemistry and Laboratory Medicine

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Background: In the diagnosis of celiac disease (CD), serum assays for anti-endomysium (EMA) and anti-transglutaminase (anti-tTG) antibodies have excellent diagnostic accuracy. However, these assays are less sensitive in young pediatric patients. Recently, a new ELISA test using deamidated gliadin peptides (DGP) as antigen has proved to be very sensitive and specific even in pediatric patients. In addition, anti-actin IgA antibodies (AAA) is another test that can be used in CD patients because antibody concentrations correlate with the degree of villous atrophy. This study evaluated the clinical accuracy of anti-tTG, EMA, AGA, anti-DGP and AAA and the effectiveness of these in different combinations for diagnosing CD in a large cohort of pediatric patients. Methods: Sera of 150 children under 6 years of age were tested: 95 patients had a diagnosis of CD, while 55 patients who did not suffer from CD were used as controls. Anti-DGP IgA/IgG and AAA were assayed with ELISA kits, while anti-tTG IgA/IgG and AGA IgG/IgA were assayed using a quantitative fluoroimmunoassay. The EMA test was conducted by indirect immunofluorescence.

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“…Several lines of evidence support retrotransport of SIgA-gliadin complexes in active CD. First, the intestinal lumen of patients with active CD contains elevated levels of antigliadin IgA antibodies ( 17 ), which can bind a set of gliadin peptides ( 42,43 ) including the 33-mer ( 44 ) and p31-55 ( 45 ). We show the presence of high molecular weight gliadin-specifi c IgA immune complexes containing Tgase.…”

Section: Iga Mediates the Intestinal Transport Of Intact 33-mer Via Cd71mentioning

confidence: 99%

Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease

Matysiak‐Budnik¹,

Moura²,

Arcos-Fajardo³

et al. 2008

J Cell Biol

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Serologic Assay Based on Gliadin-Related Nonapeptides as a Highly Sensitive and Specific Diagnostic Aid in Celiac Disease

Cited by 116 publications

References 35 publications

Diagnostic efficacy of the ELISA test for the detection of deamidated anti‐gliadin peptide antibodies in the diagnosis and monitoring of celiac disease

Diagnostic efficacy of the ELISA test for the detection of deamidated anti‐gliadin peptide antibodies in the diagnosis and monitoring of celiac disease

The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?

Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease

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