Serological Activity of Low Frequency Antigens of the MNSs System and Reappraisal of the Miltenberger Complex

Abstract

Low frequency antigens of the MNSs system were tested for sensitivity to trypsin treatment which subdivided them into two distinct groups. A new interpretation of the Miltenberger complex based on determinants rather than type sera is proposed.

Further Examples of Human Anti-M^e Found in Sera of Israeli Donors

“…N He(+) red cells and untreated M He(-) red cells removed all antibody, but trypsin-treated MHe(-) had no effect. Table II In a recent report [3] the He determinant has been located, as predicted [4], on the Ss sialoglycoprotein (SGP). At the N-terminal end the normal leucine is replaced by tryp tophan which is accompanied by glycine at position 5 instead of glutamic acid.…”

“…Unexpected positive reactions with N He(+) control red cells led to further inves tigation and identification of anti-Me in 9 samples. The report describes the serologi cal activity of the Me determinant in relation to its trypsin sensitivity, since M is sensitive [1] and He is insensitive to treatment [4], The anti-Me(M) sera were all capable of reacting in saline at 18 °C. Tests were per formed with untreated and trypsin-treated [6] red cells in tubes and given a short spin (1,000 rpm for 1 min) to enhance agglutina tion, before reading microscopically.…”

Miltenberger Subsystem of the MNSs Blood Group System

“…they are non reactive with anti-Mia. The author does not agree with the decision to ignore the reaction of sera of the Anek type with class VII RBC [19]. Since there are also difficulties in explaining the reaction pattern of serum Lane, the author has preferred to use the original names Anek, Raddon and Lane, rather than the ISBT-defined names and numThe Miltenberger (Mi) antigens represent a group of low frequency antigens belonging to the MNSs system.…”

“…Therefore, a detailed review of the serology, genetics and biochemistry of the Mi subsystem appears to be justified. The relevant studies concerning the serology and genetics of the Mi subsystem have been published in Vox Sanguinis [8][9][10][11][12][13][14][15][16][17]19].…”

An Unusual Sialoglycoprotein Associated with the Webb-Positive Phenotype

“…In addition, there exist a number of low frequency antigens associated with the MNs system which are found in association with hybrid SGPs [5], e.g. Hil [6][7][8][9], Mur [8,9], Sta [10] and Dantu [11,12]. Other low frequency antigens associated with the MNSs system are due to amino acid substitutions occurring in the normal a or 8 SGPs, e.g.…”