Serological and pathalogenic characterization of Erysipelothrix rhusiopathiae isolates from tonsils of slaughter pigs in Indonesia

Takahashi, Toshio; Zarkasie, Kamaluddin; Mariana, Siti; Sumadi, .; Ogata, M.

doi:10.1016/0378-1135(89)90029-1

Cited by 14 publications

(14 citation statements)

References 10 publications

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“…Isolation of the bacteria from the each sample, and serotyping of bacteria identified as E. rhusiopathiae were performed by the method of Takahashi et al [14].…”

Section: Bacterial Strainsmentioning

confidence: 99%

Protective Effect of NaOH-Extracted Erysipelothrix rhusiopathiae Vaccine in Pigs.

Kitajima

Oishi

Amimoto

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. A vaccine was prepared from a NaOH-extracted antigen of the Kyoto strain (serovar 2) of Erysipelothrix rhusiopathiae (E. rhusiopathiae) with an oil adjuvant, and was injected twice at 3-week intervals into SPF pigs and conventional pigs with maternal antibodies. After the second vaccination, IgG-GA titers of immunized SPF pigs were more than 256-fold at 3 weeks, and immunized pigs with maternal antibodies were 64-fold at 7 weeks. The pig with maternal antibodies vaccinated once with live vaccine had less than 4-fold titers. The ELISA antibody titers which were measured by using the NaOH-extracted antigen showed similar transition to the IgG-GA antibody titers. All immunized pigs and nonvaccinated control pigs were challenged with the strains Fujisawa (serovar 1a) or Saitama-1 (serovar 2). After challenge exposure, all pigs immunized with the NaOH-extracted vaccine showed no clinical signs and survived, and the pig immunized with the live vaccine had a local rhomboidal lesion at the site of the injection. Nonvaccinated pigs developed typical symptoms of E. rhusiopathiae infection and one of them died. After the autopsy, the challenge strains were not recovered from the main organs except tonsils of the pigs immunized with the NaOH-extracted vaccine. These results indicated that the NaOH-extracted vaccine induces a protective effect in pigs with maternal antibodies as well as in SPF pigs negative for such antibodies, and that 67-64, 62-60 kDa proteins in the NaOH-extracted antigen play an important role in protecting against E. rhusiopathiae infection. -KEY WORDS: Erysipelothrix rhusiopathiae, NaOH-extracted antigen, vaccine.

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“…Isolation of the bacteria from the each sample, and serotyping of bacteria identified as E. rhusiopathiae were performed by the method of Takahashi et al [14].…”

Section: Bacterial Strainsmentioning

confidence: 99%

Protective Effect of NaOH-Extracted Erysipelothrix rhusiopathiae Vaccine in Pigs.

Kitajima

Oishi

Amimoto

et al. 1998

The Journal of Veterinary Medical Science

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“…Pathogenicity tests: Tests for pathogenic characteristics of 29 selected isolates were conducted as described previously [18,25]. Groups of 4 mice were inoculated subcutaneously with 0.1 ml of TP broth culture (10 8 colony forming units (CFU)/ml) of each isolate.…”

Section: Methodsmentioning

confidence: 99%

Serotyping and Pathogenicity of Erysipelothrix Strains Isolated from Tonsils of Slaughter Pigs in Thailand.

Takahashi

Sunama²,

Satra³

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. Erysipelothrix strains were isolated from the tonsils of 46 (15.0%) of 307 apparently healthy slaughter pigs in Thailand during the period of August to September, 1997. A total of 27 of the 46 Erysipelothrix isolates could be classified into 5 serovars but the remaining 19 were untypable in this study. Of the 25 isolates serologically identified as Erysipelothrix rhusiopathiae, 20, 4, and 1 isolates belonged to serovars 2, 12, and 17, respectively. Only 2 isolates from the tonsils belonged to Erysipelothrix tonsillarum and represented either serovar 7 or 10. Although the periods and the districts of the survey were limited, the information obtained in the present investigation demonstrates the presence of a variety of serovars in pigs in Thailand. Of 29 selected isolates belonging to serovars 2, 7, 10, 12, 17, and untypable, only 5 (17.2%) were virulent for both mice and pigs. Five of these virulent isolates belonging to serovars 2 and 12 killed less than 30% of mice immunized with a swine erysipelas bacterin commercially available in Thailand, suggesting that the vaccine elicited a sufficient immunity to these field isolates.-KEY WORDS: characterization, Erysipelothrix, slaughter swine, Thailand, tonsil.

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“…Dentre os isolados de tonsila de suínos portadores houve maior variabilidade de sorotipos (1b, 2b, 5, 6, 7, 8, 10, 11, 12, 17, 19 e 21), sendo que entre os isolados recentes foram encontrados os sorotipos 1b, 2b, 5, 6, 8, 10 e 19, enquanto que entre os isolados históricos foram identificados os sorotipos 2b, 5,6,7,8,11,12,17,19 Foram identificados 18 sorotipos entre as amostras deste estudo (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 e 25) Em relação às amostras de referência provenientes da Austrália, a maioria dos isolados teve identificação concordante com a descrita há 30 anos (1b, 2a, 2b, 4, 5, 6, 7, 10, 11, 12 e 15 (TAKAHASHI et al, 1984a,b;TAKAHASHI et al, 1987a;TAKAHASHI et al, 1989;VENDITTI et al, 1999;YAMAMOTO et al, 1999;.…”

Section: Identificação Sorológicaunclassified

“…Até certo tempo atrás, era sugerido que a resistência nesta espécie bacteriana se desse por meio de mutações cromossomais, uma vez que nenhum plasmídeo ainda havia sido detectado em amostras de E. rhusiopathiae resistentes à canamicina, oxitetraciclina e sulfonamida (TAKAHASHI et al, 1984b (TAKAHASHI et al, 1984a,b;TAKAHASHI et al, 1989;VENDITTI et al, 1999;YAMAMOTO et al, 1999;. A tiamulina, droga que não foi testada pela disco-difusão, também obteve valores de CIM dentro da faixa de sensibilidade, resultado que corrobora com as descrições de outros autores .…”

Section: Identificação Sorológicaunclassified

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Caracterização de amostras de Erysipelothrix spp. isoladas de suínos nos últimos 30 anos

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Serological and pathalogenic characterization of Erysipelothrix rhusiopathiae isolates from tonsils of slaughter pigs in Indonesia

Cited by 14 publications

References 10 publications

Protective Effect of NaOH-Extracted Erysipelothrix rhusiopathiae Vaccine in Pigs.

Protective Effect of NaOH-Extracted Erysipelothrix rhusiopathiae Vaccine in Pigs.

Serotyping and Pathogenicity of Erysipelothrix Strains Isolated from Tonsils of Slaughter Pigs in Thailand.

Caracterização de amostras de Erysipelothrix spp. isoladas de suínos nos últimos 30 anos

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