Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 II the use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants

Kittelberger, Reinhold; Hilbink, F.; Hansen, Michael A.; Ross, G.; Joyce, Maree A.; Fenwick, Stanley G.; Heesemann, J.; Wolf‐Watz, Hans; Nielsen, Klaus

doi:10.1016/0378-1135(95)00121-2

Cited by 36 publications

(25 citation statements)

References 11 publications

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“…Although of lower intensity and frequency than that induced against the S-LPS, the immunoresponse to Brucella proteins is highly specific for brucellosis (6,7,15,35,36,60). Accordingly, double gel immunodiffusion and RID with the mixture of proteins contained in cytosolic fractions (Table 1) were 100% specific with the sera from animals infected by Y. enterocolitica O:9 or belonging to FPSR herds.…”

Section: Discussionmentioning

confidence: 94%

“…A second approach is based on the use of antigens not shared by these bacteria. The enterobacterial common antigen (40), Y. enterocolitica flagellar antigens (40), and outer membrane proteins (30,36,68) have been found to be of little usefulness, and the existence of dual infections by Y. enterocolitica O:9 and B. abortus (36,42) further reduces the value of Y. enterocolitica-specific antigens. On the other hand, the immunoresponse to Brucella proteins is highly specific (7, 8, 10-12, 13-15, 17, 18, 20, 30, 35), and on the basis of present evidence, the best available strategy to solve the FPSR problem is the use of a skin test with Brucella-soluble proteins (brucellin) (8,11,12,52,30).…”

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confidence: 99%

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Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Muñoz

Marín

Monreal

et al. 2005

Clin Vaccine Immunol

134

107

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Brucellosis is a disease caused by members of the genus Brucella that affects animals and humans. The species that infects cattle most often is Brucella abortus, but cattle infections by Brucella melitensis are not rare in areas where there is contact with infected sheep and goats (63, 64). Both B. abortus and B. melitensis are termed smooth (S) because they bear a S-type lipopolysaccharide (S-LPS). Many serological tests have been proposed for the diagnosis of brucellosis caused by S brucellae, and they can be broadly classified as those detecting antibodies to the S-LPS and those detecting antibodies to proteins (21,45). The former tests use either suspensions of S brucellae as antigens (3) or S-LPS extracts. The Rose Bengal test (RBT) and the complement fixation test (CFT) belong to the first group, and are recommended by the Office International des Épizooties for international trade (4). In addition, indirect enzyme-linked immunosorbent assays (ELISA) using S-LPS extracts or its O-chain have been extensively studied (47) and may replace the RBT and CFT. S-LPS tests are the most sensitive for detecting cattle brucellosis, but they may yield false positive results for cattle vaccinated with B. abortus

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Muñoz

Marín

Monreal

et al. 2005

Clin Vaccine Immunol

134

107

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“…rather than cross-reacting bacteria (Kittelberger et al, 1995). Differences in prevalence estimates obtained from the four tests highlight the need to consider test performance when presenting results of serologic surveys.…”

Section: Discussionmentioning

confidence: 99%

Epizootiology of Brucella Infection in Australian Fur Seals

Lynch

Duignan

Taylor

et al. 2011

Journal of Wildlife Diseases

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ABSTRACT:Novel members of the bacterial genus Brucella have recently emerged as pathogens of various marine mammal species and as potential zoonotic agents. We investigated the epizootiology of Brucella infection in Australian fur seals (Arctocephalus pusillus doriferus) by establishing demographic and temporal variations in antibody prevalence, attempting isolation of the causative agent, and determining whether this potential pathogen is involved in frequent abortions observed in this pinniped species. Two competitive enzyme-linked immunosorbent assays (cELISAs), an indirect ELISA, and a fluorescence polarization assay (FPA) were used to test sera for Brucella antibodies. The FPA and cELISA proved suitable for use in this species. Significant differences in antibody prevalence were found between age classes of seals sampled between 2007 and 2009 at one colony. Pups sampled at this site (n5134) were negative for Brucella antibodies by all serologic tests but 17 of 45 (38%) of juveniles were antibody-positive. Antibody prevalence in adult females was significantly higher than in juveniles (P50.044). . Inflammatory lesions suggestive of infectious agents were found in 14 of 39 aborted Australian fur seal pups, but pathologic changes were not uniformly consistent for Brucella infection. Culture and PCR investigations on fetal tissues were negative for Brucella. Culture and PCR on selected fresh or frozen tissues from 36 juvenile and adult animals were also negative. We suspect that the prevalence of active infection with Brucella in Australian fur seals is low relative to antibody prevalence.

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“…The cross-reactivity of Yersinia with other bacterial species, such as Borrellia burgdorferi (3,25), Rickettsia rickettsii (2,23), and Brucella spp. (2,(17)(18)(19), has been reported. Additionally, crossreactivity between Yersinia and thyroid-stimulating immunoglobulin (TSI) in patients with Graves ' disease has been shown (1,2,13,24).…”

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confidence: 99%

Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi

Rawlins

Gerstner

Hill

et al. 2005

Clin Vaccine Immunol

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Yersinia enterocolitica and Yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. We evaluated a Western blot assay which uses Yersinia outer membrane proteins as antigens for the detection of Yersinia antibodies as a replacement for the complement fixation (CF) assay. Clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the CF assay, Western blot assay, and enzyme-linked immunosorbent assay (ELISA). The CF assay and ELISA were compared to the Western blot assay, which was the reference method used in this study. Sera with antibodies that could potentially cross-react with Yersinia were also tested by the Western blot assay. The agreement, sensitivity, and specificity of the CF method were 61%, 26%, and 95%, respectively; and those for the ELISA were 89%, 95%, and 82%, respectively. The prevalences of Yersinia antibodies in 50 healthy donors were 6% for immunoglobulin G (IgG), 2% for IgA, and 2% for IgM. Sera positive for Bartonella henselae, Brucella, Chlamydia pneumoniae, and Rickettsia rickettsii antibodies showed cross-reactivity by the Western blot assay. The highest cross-reactivity was observed with Borrelia burgdorferi; 5 of 11 (45%) specimens were cross-reactive by the IgM-specific assay. Overall, the Western blot assay performs acceptably and is more sensitive than the CF assay, warranting replacement of the CF assay in the laboratory. Due to the evidence of cross-reactivity, particularly with B. burgdorferi, which can cause an oligoarthritis similar to reactive arthritis, the diagnosis of reactive arthritis should be based on clinical findings and complete serologic analysis of the potential causative infectious pathogens.

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Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 II the use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants

Cited by 36 publications

References 11 publications

Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Epizootiology of Brucella Infection in Australian Fur Seals

Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi

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