Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

Abstract

The two commercially available ELISA systems allow for a highly sensitive and specific diagnosis of EBA. The sensitivity of the Col7-NC1/NC2 ELISA is significantly higher compared with the ELISA based on the Col7-NC1 domain only.

Diagnosis of anti‐laminin γ‐1 pemphigoid by immunoblot analysis

“…Preparation and evaluation of immunofluorescence microscopy on salt-split human skin was performed as previously described. 27…”

“…IgG serum autoantibodies against BP180, BP230 and ColVII were excluded using commercially available ELISA (Euroimmun, L€ ubeck, Germany); according to the manufacturers' protocol. 27 Presence of autoantibodies against laminin 332 were excluded by ELISA using native human laminin 332 as previously described. 28…”

Accessible diagnostic methods to differentiate between epidermolysis bullosa acquisita and other subepidermal autoimmune bullous diseases

“…The sensitivity of IIF microscopy on human salt-split skin for the detection of anti-BMZ autoantibodies in EBA ranged between 27% and 100% in several studies, with a pooled measure of 75.9% in 426 EBA sera reported so far. [4]…”

“…concluded that type VII collagen combined NC1/NC2 domains ELISA is superior to ELISA based on type VII collagen NC1 domain only, immunoblotting, and IIF on salt-split skin, with a sensitivity of 97.9%. [4] All of the immunoassays above are restricted to several specialized laboratories across the world.…”

Improving the diagnosis of autoimmune blistering dermatoses of the oral mucosa

“…5 Other causes of mucosal erosions and ulcerations include other AIBD, such as laminin c-1 pemphigoid and epidermolysis bullosa acquisita, Stevens-Johnson syndrome, erythema multiforme and erosive lichen planus (LP). 6,7 Of note, cases of oral MMP with clinical and histopathological characteristics of oral LP have been highlighted recently. 8 Moreover, coexistence of lichenoid mucositis with acantholysis is typically found in paraneoplastic pemphigus.…”