Serological differentiation between infected and vaccinated cattle by visual and quantitative immunofluorescence using Brucella abortus antigen coupled sepharose beads

Raybould, T. J. G.; Chantler, Shireen

doi:10.1016/0022-1759(79)90271-0

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…Indirect primary binding assays usually rely on antibody present in test serum (or other body fluids) reacting with immobilized antigen and then being detected using a detection system with a marker molecule. The tracer system varies from antiglobulins labelled with isotopes [74-76, 78, 89-95] to fluorochromes [96][97][98][99][100][101][102][103][104][105][106][107][108][109][110] to enzymes (described initially by Carlsson et al, 1976 [111] and reviewed by Nielsen and Gall, 1994 [112-141].…”

Section: Indirect Formatsmentioning

confidence: 99%

Diagnosis of Brucellosis

Poester¹,

Nielsen²,

Samartino³

et al. 2010

TOVSJ

111

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Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis, and orchitis are the most common clinical manifestations in animals. In humans, brucellosis is a debilitating and chronic disease, which may affect a variety of organs. Clinical diagnosis of brucellosis is not easily achieved. Laboratory testing is therefore very important for a correct identification of the disease in humans and for the detection and confirmation in animals. Definitive diagnosis is normally done by isolation and identification of the causative agent. While definitive, isolation is time-consuming, must be performed by highly skilled personnel, and it is hazardous. For these reasons, serological tests are normally preferred. Brucellosis serology have advanced considerably in the last decades with very sensitive and specific new tests available. Modern genetic characterization of Brucellae using molecular DNA technology have been developed. Several PCR-based assays have been proposed, from the rapid recognition of genus to differential identification of species and strains. This review describes bacteriological, serological, and molecular methods used for the diagnosis of human and animal brucellosis.

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Section: Indirect Formatsmentioning

confidence: 99%

Diagnosis of Brucellosis

Poester¹,

Nielsen²,

Samartino³

et al. 2010

TOVSJ

111

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“…Previous studies have indicated that antigen X is responsible for the differential reactivity of bovine sera from infected and vaccinated animals in immunofluorescence and passive HA systems utilizing SDS extracts of B. abortus 544/W (20,21). Differential distribution of antigen X among virulent and vaccine strains of B. abortus might therefore have been anticipated.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial cultures. Bacterial cultures of B. abortus 544/W for immunoenzyme studies were prepared as previously described (20).…”

Section: Methodsmentioning

confidence: 99%

“…Representative virulent and vaccine strains of biotypes 1 to 7, and 9 of B. abortus were obtained from the National Culture Type Collection, (NCTC) Colindale, the Cen-tral Veterinary Laboratory (CVL), Weybridge, and Culture Collection, the Wellcome Research Laboratories, Beckenham. Ten percent suspensions of each culture from erythritol-supplemented serum dextrose agar were extracted with 0.5% SDS (20).…”

Section: Methodsmentioning

confidence: 99%

“…Recent immunofluorescence (20) and passive hemagglutination (HA) studies (21) using sodium dodecyl sulfate (SDS) extracts of B. abortus 544/W suggest that selected antigens enable improved serodiagnosis of bovine brucellosis compared to conventional bacterial cell agglutination tests.…”

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confidence: 99%

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Ultrastructural localization of characterized antigens of Brucella abortus and distribution among different biotypes

Raybould¹,

Beesley²,

Chantler³

1981

Infect Immun

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The distribution of characterized antigens in extracts of representative strains of Brucella abortus biotypes 1 to 9 was investigated by hemagglutination and precipitation reactivity. An antigen designated X was present in high concentrations in sodium dodecyl sulfate extracts of all smooth strains examined, but in low concentrations in the rough strain 45/20 extract. Antigen beta was demonstrable in 8 out of 12 smooth strains of B. abortus, but antigen gamma was detected in only 3 of these strains. Vaccine strain 19 extract contained beta antigen but no detectable gamma antigen, whereas both these antigens were present in rough strain 45/20 at slightly lower concentrations that in strain 544/W extract. Immunoenzyme histochemical studies performed with an electron microscope showed that antigens beta, gamma, and X were all situated in the outer membrane envelope of the cell wall of B. abortus 544/W.

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