Serological Evidence of Dengue and West Nile Virus Human Infection in Juarez City, Mexico

Palermo, Pedro M.; Mora-Covarrubias, Antonio de la; Jiménez-Vega, Florinda; Watts, Douglas M.

doi:10.1089/vbz.2018.2302

Cited by 7 publications

(12 citation statements)

References 40 publications

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“…Of the 11 mothers, 6 had not traveled outside of El Paso, Texas, and 3 had traveled to Mexico, one to Puerto Rico, and one had traveled, but not to a DENV endemic country. These observations and the more recent evidence that supported endemic transmission among a human cohort with DENV infection in Ciudad Juarez, MX, the urban adjoining sister community with El Paso suggested that the mothers could have acquired DENV infections in the El Paso community [12].…”

Section: Discussionmentioning

confidence: 77%

“…Seroprevalence rates as evidence of past DENV infection ranged from 23% in Laredo, TX to 38% in Brownsville, TX [8,37]. More recently, observations indicated that during 2015, 14.1% of a selected cohort of 77 adults had serological evidence of a past DENV infection and 10.2% (5/61) seroconverted as evidence of a recent DENV 1 and 2 infection the El Paso adjoining sister community of Ciudad Juarez, MX [12]. Other than the Rio Grande valley region of TX, autochthonous transmission of DENV has not been confirmed for any other U.S. border communities [13].…”

Section: Discussionmentioning

confidence: 99%

“…As a result, it may be possible that DENV are causing unrecognized human infections in the El Paso community (Watts D.M., unpublished data). Also, the more recent evidence that DENV was endemic in Ciudad Juarez, Mexico, an adjoining urban community to El Paso raised awareness of the possibility that these viruses are endemic in El Paso community [12]. These observations underscores the critical need for implementing and sustaining active surveillance program for understanding the endemic/enzootic arboviruses, as well as for the early detection of introduced viruses.…”

Section: Introductionmentioning

confidence: 99%

“…Outbreaks of DF have also been reported from 2007 to 2014 along the US-MX border in Sonora, MX, including 93 cases during 2014 in Yuma, Arizona, however, all infections were apparently acquired on the MX side of the border [11]. More recently, a report indicated that 14.1% of a selected cohort of 77 adults had serological evidence of a past DENV infection and 10.2% (5/ 61) had evidence of a recent DENV 1 and 2 infection during 2015 in the El Paso adjoining sister community of Ciudad Juarez, MX [12]. Although Ae.…”

Section: Introductionmentioning

confidence: 99%

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Serosurvey for dengue virus infection among pregnant women in the West Nile virus enzootic community of El Paso Texas

et al. 2020

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All 4 dengue viruses (DENV) cause sporadic outbreaks of human disease in the Rio Grande Valley along the US-Mexico border. In addition, West Nile virus (WNV) is enzootic in most border communities, and is the only arbovirus known to cause human disease in the El Paso, Texas community. In an effort to determine if DENV were also endemic in the El Paso community, a serosurvey was conducted among mothers at the time of delivery of their babies in selected hospitals. Cord-blood plasma samples obtained from mothers were tested for DENV antibody by an enzyme-linked immuno-sorbent assay (ELISA), plaque reduction neutralization test (PRNT) and a multiplex microsphere immunoassay. All DENV antibody positive plasma samples were also tested for WNV antibody by the same assays to consider the possibility that DENV antibody positive samples reflected WNV cross reactive antibody. The results indicated that 0.74% (11/1,472) of the mothers had a previous DENV infection and that 3.3% (48/1,472) had a previous WNV infection. Of these mothers, 0.20% (3/1,472) had antibody to both DENV and WNV as evidence of infection by both viruses. The results indicated that 0.2% (3/1472) of the mothers were positive for antibody to only WNV envelope, thus suggesting an undetermined flavivirus infection. Although 6 of the 11 DENV antibody positive mothers did not have a history of travel to a DENV endemic country, the findings of this survey provided further evidence of local transmission of WNV and suggested the possibility of focal autochthonous transmission of DENV in the El Paso community.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serosurvey for dengue virus infection among pregnant women in the West Nile virus enzootic community of El Paso Texas

et al. 2020

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“…Indirect immunoglobulin G enzyme-linked immunoabsorbent assay WTD sera samples were tested for immunoglobulin G (IgG) antibody to WNV by an indirect enzyme-linked immunosorbent assay (ELISA) using a lysate of Vero cells as previously described (Palermo et al 2019) with slight modifications. In brief, sera samples were diluted at a 1:100 di-lution in blocking buffer (5% skim milk, 1% Tween, in phosphate-buffered saline [PBS] 1X pH 7.4) and tested in duplicate against WNV antigen prepared as infected Vero cell lysates, and uninfected lysate cells as a control antigen.…”

Section: Sample Collectionmentioning

confidence: 99%

Serological Evidence of West Nile Virus Infection in White-Tailed Deer in Central Texas

Palermo

Orbegozo

Morrill

et al. 2020

Vector-Borne and Zoonotic Diseases

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White-tailed deer (WTD) are abundant mammals widely distributed across the United States. As a result, WTD are considered to be excellent sentinels for detecting arboviral activity in certain geographic areas. Evidence of West Nile virus (WNV) antibody in WTD has been reported previously in several states. However, WNV infection in WTD has not been reported from Texas, where the incidence of human West Nile (WN) cases is among the highest in the United States. Therefore, the aim of this study was to determine the prevalence of WNV antibody in WTD in central Texas. Sera samples (n = 644) were collected from deer during the fall and winter in western Travis County, Texas from 2014 to 2018 and tested for WNV immunoglobulin G (IgG) antibody by an indirect enzyme-linked immunosorbent assay (ELISA). ELISA antibody-positive samples were further tested for WNV and St. Louis encephalitis virus (SLEV) antibodies by an 80% plaque-reduction neutralization tests (PRNT 80). Overall, 9% (n = 58) and 0.31% (n = 2) of the deer samples had serological evidence of WNV and SLEV infections, respectively. WNV seroprevalence differed significantly by age (p < 0.05), but there was no significant difference between sex. Interestingly, 3.1% (n = 20) of the samples were positive for Flavivirus IgG antibody by ELISA, but negative for SLEV and WNV antibodies, suggesting that other Flaviviruses may be circulating among WTD in Texas. Finally, these results supported WNV infection among WTD and highlight their potential role as sentinels for the detection of WNV in Texas and warrant further studies to determine the role WTD play in the maintenance and transmission of WNV.

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West Nile virus in Mexico: Why vectors matter for explaining the current absence of epidemics

Correa-Morales,

González-Acosta,

Ibarra-Ojeda

et al. 2024

Acta Tropica

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Serological Evidence of Dengue and West Nile Virus Human Infection in Juarez City, Mexico

Cited by 7 publications

References 40 publications

Serosurvey for dengue virus infection among pregnant women in the West Nile virus enzootic community of El Paso Texas

Serosurvey for dengue virus infection among pregnant women in the West Nile virus enzootic community of El Paso Texas

Serological Evidence of West Nile Virus Infection in White-Tailed Deer in Central Texas

West Nile virus in Mexico: Why vectors matter for explaining the current absence of epidemics

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