Serological study of small ruminant lentivirus in goats in Italy

Gufler, H.; Gasteiner, J.; Lombardo, D.; Stifter, Ernst; Krassnig, R; Baumgartner,

doi:10.1016/j.smallrumres.2007.01.016

Cited by 20 publications

(15 citation statements)

References 19 publications

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“…CAEV control has remained a big challenge for the goat industry, as prophylactic vaccinations do not induce antibodies that result in efficient viral clearance and provide protection against arthritis [11]. The control measures therefore rely heavily on accurate and reasonable laboratory diagnosis to identify and cull the CAEV-infected subjects so as to reduce economic losses [2].…”

Section: Discussionmentioning

confidence: 99%

“…CAEV infection is one of the most destructive and economically important viral diseases of the goat industry and is spread throughout many countries of the world, including the United States (31 %) [30], Norway (86 %) [22], Great Britain (54.5 %) [32], Switzerland (26.9 %) [3], Spain (20.6 %) [31], Poland (12.1 %) [15], Italy (6.58 %) [11], Japan (63.3 %) [14], Mexico (56.8 %) [33], Brazil (35 %) [18], Jordan (23.2 %) [1], Korea (2.73 %) [23] and China (0.2 %–30 %) [26]. Overall, the live-animal trade and exportation of goats play a major role in CAEV dissemination across large geographical regions [24].…”

Section: Introductionmentioning

confidence: 99%

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Development of TaqMan-based qPCR method for detection of caprine arthritis–encephalitis virus (CAEV) infection

Zhou

et al. 2013

Arch Virol

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A specific and sensitive two-step TaqMan real-time PCR has been developed for rapid diagnosis of caprine arthritis-encephalitis virus (CAEV) infection by using a set of specific primers and a TaqMan probe targeting a highly conserved region within the gene encoding the viral capsid protein (CA). The assay successfully detected CAEV proviral DNA in total DNA extracts originating from cell culture, whole blood samples and isolated PBMCs, with a lower detection limit of 102 copies and a linear dynamic range of 105 to 1010 copies/ml. There was no cross-reaction with other animal viruses (e.g., goat pox virus, bovine leukemia virus, bovine mucosal disease virus, swine influenza virus and Nipah virus). When applied in parallel with serological AGID and conventional PCR for detection of CAEV in field samples, this assay exhibited a higher sensitivity than these traditional methods, and 7.8 % of the 308 specimens collected in the Shanxi and Tianjin regions of China from 1993 to 2011 were found to be positive. Thus, the TaqMan qPCR assay provides a fast, specific and sensitive means for detecting CAEV proviral DNA in goat specimens and should be useful for large-scale detection in eradication programs and epidemiological studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of TaqMan-based qPCR method for detection of caprine arthritis–encephalitis virus (CAEV) infection

Zhou

et al. 2013

Arch Virol

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“…Both asymptomatic and diseased animals are a source of viruses for naive animals [8,9,10,11,12,13,14,15,16]. …”

Section: Small Ruminant Lentiviruses (Srlvs)mentioning

confidence: 99%

Retroviral Infections in Sheep and Goats: Small Ruminant Lentiviruses and Host Interaction

Larruskain

Jugo

2013

Viruses

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Small ruminant lentiviruses (SRLV) are members of the Retrovirus family comprising the closely related Visna/Maedi Virus (VMV) and the Caprine Arthritis-Encephalitis Virus (CAEV), which infect sheep and goats. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Infection by VMV and CAEV can lead to Visna/Maedi (VM) and Caprine Arthritis-Encephalitis (CAE) respectively, slow progressive inflammatory diseases primarily affecting the lungs, nervous system, joints and mammary glands. VM and CAE are distributed worldwide and develop over a period of months or years, always leading to the death of the host, with the consequent economic and welfare implications. Currently, the control of VM and CAE relies on the control of transmission and culling of infected animals. However, there is evidence that host genetics play an important role in determining Susceptibility/Resistance to SRLV infection and disease progression, but little work has been performed in small ruminants. More research is necessary to understand the host-SRLV interaction.

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“…The following ones can be mentioned as susceptible: Ovis aries, Capra aegagrus hircus, Capra ibex, Ovis gmelinii, Oreamnos americanus, Ovis orientalis, and Ovis aries musimon (Denner, 2007;Guiguen et al, 2000;Leroux et al, 2010;Ramirez et al, 2013;Sanjose et al, 2016). SRLVs transmission can be accomplished under the following conditions: direct contact (Leginagoikoa et al, 2006;Pisoni et al, 2007;Pérez et al, 2010), feeding with infected colostrum/milk (Mdurvwa et al, 1994;Leginagoikoa et al, 2006;Villoria et al, 2013;Junkuszew et al, 2016;Yang et al, 2017) or feeding on an infected pasture (Gufler, 2004;Gufler et al, 2007). The sexual transmission is not excluded (Al Ahmad et al, 2005;Lamara et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Serological Results of MVV and Caev Antibodies in Samples Collected From Sheep and Goat Flocks of Romania

Enache

Bărăităreanu

Dan

et al. 2019

CBUP

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INTRODUCTION: Maedi-Visna (MV) and Caprine Arthritis Encephalitis (CAE) are specific diseases of small ruminants caused by lentiretroviruses. These diseases are not a public health concern but they are important due to their economic impact. OBJECTIVES: The aim of this study is to analyse the results of the serological screening for MVV and CAEV antibodies in samples collected from small ruminants farms located in 14 Romanian counties and the proximity of Bucharest city. METHODS: The samples were analysed by indirect ELISA, using a commercial diagnosis kit. We investigated 702 serum samples in 160 iELISA tests: 148 pools (2-5 samples/pool) and 12 individual animals. RESULTS: We noticed 27.50% positive samples that confirm the exposure to MVV and CAEV viruses of small ruminants, but the confirmation is requesting investigation using molecular biology tools or other serological techniques. CONCLUSION: A reliable picture over the SRLV infections could underlie a national program for monitoring. UDC Classification: 616.9,

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Serological study of small ruminant lentivirus in goats in Italy

Cited by 20 publications

References 19 publications

Development of TaqMan-based qPCR method for detection of caprine arthritis–encephalitis virus (CAEV) infection

Development of TaqMan-based qPCR method for detection of caprine arthritis–encephalitis virus (CAEV) infection

Retroviral Infections in Sheep and Goats: Small Ruminant Lentiviruses and Host Interaction

Serological Results of MVV and Caev Antibodies in Samples Collected From Sheep and Goat Flocks of Romania

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