Serological typing of reference strains and clinical isolates of streptococcus mutans by agglutination reactions and/or radioimmunoassay

Ota, Fusao; Kiso, Michiko; Akiyama, Yasuyuki; Satomi, Yasuhiko; Yasuoka, Masahito; Fukui, Komei

doi:10.1016/s0176-6724(87)80251-1

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…All these results indicate that a combination of both antibodies with the previous ones [Ota et al, 1987b] can successfully identify serotypes d, g and h of S. sobrinus and S. downei. Moreover, with the previous observations [Kato et al, 1986], the results support the existence of subserotypes or serotypes, as suggested for serotype c [Ota et al, 1987a]. In fact, a new subtype of S. mutans (serotype k) [Nakano et al, 2004] has been reported very recently.…”

Section: Serological Diversity Of Eight Serotypes Of Mutans Streptococcisupporting

confidence: 90%

“…Copyright © 2006 S. Karger AG, Basel Species belonging to the mutans group of streptococci have been considered responsible for dental caries for many years [Fitzgerald, 1960;Hamada, 1986;Loesche, 1986]. They have been divided into 8 serotypes, a-h, based on the serological specifi city of cell wall antigens usually using polyclonal antibodies [Hamada, 1986;Ota et al, 1985Ota et al, , 1987a. Several authors have prepared monoclonal antibodies (mAbs) reacting with some types of mutans streptococci.…”

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confidence: 99%

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Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Ota

Ota²,

Mahmud³

et al. 2005

Caries Res

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A set of monoclonal antibodies were prepared by the conventional cell fusion of myeloma cells (SP2/0-Ag14) with spleen cells from BALB/c mice immunised with whole cells of a strain of mutans streptococci. Their specificities were examined against 35 reference strains of mutans streptococci, 34 reference strains of other oral streptococci and 8 reference strains of other microorganisms often inhabiting the oral cavity. Specificity was examined by enzyme immunoassay using whole cells. A total of 52 strains, consisting of 19 strains isolated in Japan, 19 strains isolated in Italy and 14 strains isolated in England, were characterised by conventional physiological and biochemical tests and then serotyped by the use of 8 monoclonal antibodies with different specificities. They were also confirmed by guanine-plus-cytosine contents of their nucleic acid and DNA-DNA hybridisation test. The results indicated that all monoclonal antibodies are useful for identification of 8 serotypes of the mutans streptococci responsible for dental caries. They also suggest the existence of more serological varieties among mutans species.

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Section: Serological Diversity Of Eight Serotypes Of Mutans Streptococcisupporting

confidence: 90%

mentioning

confidence: 99%

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Ota

Ota²,

Mahmud³

et al. 2005

Caries Res

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“…Samples were centrifuged at 2000 x g in a microcentrifuge for 5 rain to separate serum from the clot and stored in aliquots at -70 ° C. Since blood from 1 mouse on the 5 or 7% casein (w/w) diet was often too small in quantity, serum was pooled from 2-3 mice. Immunoglobulins reactive with whole cells of group B streptococci were estimated by enzyme immunoassay (EIA) by the method described previously [12]. Peroxidaselabelled anti-mouse IgG or IgM (affinity-purified, Kirkegaard and Perry Laboratories Inc., Gaithersburg, MD, U.S.A.) and HzO 2 plus ABTS (2,2'-azino-di-(3-ethyl-benzothiazolin-6-sulfonate, Kirkegaard and Perry Laboratories) were used.…”

Section: Determination Of Serum Immunoglobulins Reactive With Group Bmentioning

confidence: 99%

“…After 5 days the cultures were centrifuged and the supernate used as samples. Antibody titres in the samples were examined by EIA [12]. Control animals did not receive bacteria.…”

Section: Immunoglobulin Production From Spleen Cell Culturesmentioning

confidence: 99%

“…0.1 ml-10 g-1 body weight of an immune rabbit serum prepared by injecting living bacterial cells [12]. The immune serum was examined for its titre by agglutination reactions in microtitre plates (U-shaped wells, Titertek, VA., U.S.A.) by the method described previously [12]. The antiserum was inactivated by treatment at 56 °C for 30 min before use.…”

Section: Protection By Immune Rabbit Serummentioning

confidence: 99%

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Effects of protein intake on immune parameters in mice surviving streptococcal infection

Ota

1992

FEMS Microbiology Letters

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Groups of mice were fed for 2 weeks on isocaloric diets containing 5, 7, 20 and 40% (w/w) casein, respectively, then injected intraperitoneally with group B streptococci, and observed for their survival rates. The mice fed 7% or 20% casein had lower mortalities than those fed 5% or 40% casein. In order to explain the different survival rates, other groups of mice were fed on the experimental diets and examined for the number of leukocytes in the blood, of spleen cells and thymocytes, for IgG and IgM antibody titres to the streptococci, for haemolytic titres of sera, the amount of complement component 3 (C3), for chemiluminescence and opsonic activity of peritoneal exudate cells (PEC) and spleen cells (SP), production of superoxide anion from PEC and SP, and production of immunoglobulins from cultured SP. After 2 weeks on a 7 or 20% casein diet mice showed increased serum levels of IgM antibodies reactive with the whole bacterial cells on days 3–5 when they were immunised with a sublethal dose of group B streptococci. The mice fed on the 7% casein diet also showed a higher C3 titre than the other diet groups when assayed by enzyme immunoassay. Furthermore, opsonophagocytic activity was highest when PEC or SP taken from mice on the 20% case in diet were incubated with radiolabelled microorganisms in the presence of fresh serum taken from the 7% casein group. The production of superoxide anions from PEC and SP was lowest in the mice fed on 5% casein when activity was expressed as nano‐mol per animal. It is suggested from these results that the greater activity of phagocytic cells in the presence of increased amounts of C3 and IgM explains the heightened resistance in the mice fed on a 7% casein diet, and that suppressed opsonophagocytic activity resulting from the decreased number of leukocytes in the blood and other phagocytic cells explains the lowest resistance in the 5% casein group. However, mice fed on a 40% casein diet showed all these immunological parameters untouched, and their lowered resistance could not be explained. Different factors seem to be operative in them.

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Immunochemical analysis and localisation of a cell wall antigen of Streptococcus rattus with a specific monoclonal antibody

Ota¹,

Kato²,

Hirota

et al. 1995

Zentralblatt für Bakteriologie

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Serological typing of reference strains and clinical isolates of streptococcus mutans by agglutination reactions and/or radioimmunoassay

Cited by 7 publications

References 18 publications

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Effects of protein intake on immune parameters in mice surviving streptococcal infection

Immunochemical analysis and localisation of a cell wall antigen of Streptococcus rattus with a specific monoclonal antibody

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