Seroreactivity to specific antigens of Helicobacter pylori infection is associated with an increased risk of the dyspeptic gastrointestinal diseases

Abstract

We confirm that the universal presence of CagA and VacA in H. pylori-infected patients in Thailand is independent of the gastroduodenal disease. The presence or absence of antibodies to H. pylori-specific antigens may be useful as indirect markers in the screening of H. pylori-infected patients, and may have specific protection roles in H. pylori-related gastroduodenal diseases.

Seroreactivity to Helicobacter pylori Antigens as a Risk Indicator of Gastric Cancer

“…Having used this assay in our comparative study, we found the absolute majority of our study population to be H. pylori seropositive. Over ninety percent of whom were CagA (116kDa) seropositive in accordance with our previous genotyping studies on Iranian H. pylori strains as well as serology findings from other developing countries such as Northeastern Thailand (Chomvarin et al, 2009). In agreement with the latter study and others (Hoang et al, 2006), we found the least seropositivity toward the 35kDa protein.…”

“…We have, therefore, analyzed our data by multivariate logistic regression model adjusting for the three mentioned potential confounder and found immunoreactivity towards H. pylori 35kDa (low molecular weight) protein drastically reduced the risk of GC by 60 percent. Accordingly, in a northeast Thai population, absence of reactivity to this protein in the presence of anti 19.5kDa protein was reported as a risk marker for gastric cancer (Chomvarin et al, 2009). Conversely, in another study from Thailand (Suriani et al, 2008) a risk inducing role was reported for H. pylori low molecular weight (35 and 37kDa) antigens in the development of gastrointestinal complications including gastric ulcers and cancer respectively.…”

“…Helicoblot 2.1 kit is a qualitative western blotting assay previously used by several groups of investigators (Vilaichone et al, 2003;Rocha et al, 2004;Yang et al, 2006;Simán et al, 2007;Mitchell et al, 2008;Chomvarin et al, 2009) for assessment of H. pylori infection as well as serologic typing of the infecting strains. Having used this assay in our comparative study, we found the absolute majority of our study population to be H. pylori seropositive.…”

Colonization by Helicobacter pylori of leprosy patients in Spain: immunomodulation to low molecular weight antigens of H. pylori

“…Further studies of the serological response in 182 subjects with H. pylori infection confirmed this association in Africa (Lamarque et al 1999), Thailand (Vilaichone et al 2003) and France (Delchier et al 2001). An additional study has demonstrated that the simultaneous presence of a 19.5 kDa antibody and absence of the antibody to the 35 kDa antigen was associated with an increased risk of gastric cancer Chomvarin et al (2009)…”

Serum IgG antibodies against Helicobacter pylori low molecular weight antigens 50kDa, 30kDa and Urease A 26 kDa, along with vacuolating cytotoxin A are associated with the outcome of the infection

“…Serum antibodies against low molecular-weight-antigens as 19.5kDa [13][14][15][16][17][18][19][20][21][22] , 26.5kDa [13][14][15][16][20][21][22][23] , 30kDa [13][14][15][16][17][18][19][20][21]23 , 35kDa 13-19, 21, 24 and 60kDa 13-15, 20, 25, 26 were associated with serious outcome of H. pylori infection in some studies, but the results were conflicting, too. Less extensive investigations of serum antibodies with conflicting results were performed including 37 kDa [17][18][19] and 45 kDa 14 , 54kDa 24 , Hsp60 25,26 antigens. One study was done for serum antibodies against 46kDa 24 , 48kDa 24 , 50kDa 27 , 53kDa 22 , 57kDa 20 , 67kDa 27 antigens.…”