Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis

Ishida, Yoshiteru; Hashiguchi, Hiroyuki; Todaka, Kazunari; Kuwahara, Itsumi; Ishizuka, Yuta; Nakane, Hideyuki; Uchimura, Daisuke; Nishimori, Toshikazu; Mitsuyama, Yoshio

doi:10.1016/s0006-8993(97)01541-2

Cited by 23 publications

(16 citation statements)

References 23 publications

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“…It has been reported that the basal DA output in the cACC is not significantly different from that in the sACC (29). The concentrations of DA and DOPAC that were detected in this study are similar to those reported in the striatum (30). Moreover, the steady DA level reported in this study is close to that reported in the sACC (31), but higher than that reported in the whole ACC (32).…”

Section: Discussionsupporting

confidence: 86%

Role of Dopaminergic System in Core Part of Nucleus Accumbens in Hyperlocomotion and Rearing Induced by MK-801 in Rats: A Behavioral and In Vivo Microdialysis Study

Hatip‐Al‐Khatib

Bolukbasi

Mishima

et al. 2001

Japanese Journal of Pharmacology

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ABSTRACT-We investigated modification of the MK-801 effect on motor activity and extracellular amines concentration by 6-hydroxydopamine (6-OHDA)-induced lesion of core nucleus accumbens (cACC) of rats. In vivo microdialysis-HPLC showed that the concentrations (fmol /ml) of dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and serotonin were 0.738 ± 0.135, 155.34 ± 41.01 and 0.334 ± 0.024, respectively, in the cACC of intact rats. The DOPAC /DA ratio was 264.24 ± 94.01. Unilateral lesion of the cACC with 6-OHDA (8 mg/ml) substantially reduced DA (-93%) and DOPAC (-97%) in desipramine (30 mg /kg, i.p.)-pretreated rats (6-OHDA+DMI rats) as compared to the 65% reduction rate of both amines in salinepretreated rats (6-OHDA+saline rats). Moreover, DOPAC was reduced by 72% in 6-OHDA+DMI rats. MK-801 increased DOPAC (426 -467%) and DOPAC /DA ratio (180 -230%) in intact rats. On the other hand, MK-801 increased DA by 154% and 505% in 6-OHDA+saline and 6-OHDA+DMI rats, respectively. 6-OHDA reduced the effect of MK-801 on DOPAC and DOPAC /DA ratio. In the behavioral studies, MK-801 (0.01 -0.3 mg /kg, i.p.) increased locomotor activity and rearing of intact rats. Bilateral 6-OHDA+DMI lesion of the cACC caused greater reduction in the effect of MK-801 (0.1 mg /kg) than that of the shell nucleus accumbens. These results suggest that increased extracellular DOPAC concentration (but not DA) and DOPAC /DA ratio in the cACC plays an important role in MK-801-hyperactivity.Keywords: MK-801, Core nucleus accumbens (rat), Microdialysis, Motor activityThe nucleus accumbens (ACC) is a part of the mesocortical and mesolimbic dopaminergic system. The ACC receives dopaminergic inputs from the ventral tegmental area (VTA) and glutamatergic inputs that originate from cortical regions (including medial frontal), amygdala and midline thalamus (1). The ACC is involved in a variety of behavioral activities. The dopaminergic system in the ACC plays an important role in control of spontaneous, psychostimulants (2)-and MK-801 (3 -6)-induced locomotor hyperactivity. On the other hand, the allocortical-originated glutamatergic afferents could modulate the psychomotor activation and drug reinforcement (7). Moreover, the glutamatergic and dopaminergic systems not only separately alter the behavioral effects but could also act by interacting and modulating the function of each other. A functional interaction has been reported between the glutamatergic system (via Nmethyl-D-aspartate (NMDA) receptors) and the dopaminergic system at the striatal levels (8) with a negative modulatory effect of the glutamatergic system on the dopaminergic one (9). The consequence of glutamate block depends on which of glutamate receptors are blocked. In the ACC, it is suggested that antagonists that block only NMDA autoreceptors increase synaptic glutamate level and disinhibit dopaminergic terminals. On the other hand, NMDA and non-NMDA antagonists that block both the glutamate autoreceptors and postsynaptic receptors inactivate the dopaminergic terminals (10).Cytoarchite...

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Section: Discussionsupporting

confidence: 86%

Role of Dopaminergic System in Core Part of Nucleus Accumbens in Hyperlocomotion and Rearing Induced by MK-801 in Rats: A Behavioral and In Vivo Microdialysis Study

Hatip‐Al‐Khatib

Bolukbasi

Mishima

et al. 2001

Japanese Journal of Pharmacology

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“…2) and was consistent with previous findings. 26,28) We also confirmed the induction of AIMs by repeated L-DOPA treatment in 6-OHDA-lesioned rats in the present and previous studies. 13) This induction may be due to the facilitation of neurotransmission of DA synthesized from exogenous L-DOPA in the unilateral DA-depleted striatum by 6-OHDA; in the striatum on the lesion side, DA was synthesized in 5-HT neurons instead of DA neurons because 5-HT neurons contain AADC, an enzyme for DA synthesis.…”

Section: Discussionsupporting

confidence: 90%

“…Animals that made at least 5 turns/min with the methamphetamine challenge were selected and used to evaluate subsequent L-DOPA-induced involuntary movements. We previously reported the immediate and almost complete destruction of DA neurons in the substantia nigra and ventral tegmental area, resulting in the near total depletion (2% of normal) of DA in the striatum ipsilateral to the 6-OHDA injection, 28) using methods similar to those used in the present study.…”

Section: Methodsmentioning

confidence: 61%

“…Coronal serial sections (thickness: 50 µm) including the striatum, substantia nigra, or dorsal raphe nuclei were prepared for immunohistochemical staining. 28) Sections for tyrosine hydroxylase (TH) or 5-HT immunohistochemical staining were incubated in 10% normal horse or goat serum for 1 h at room temperature, and incubated at 4°C overnight with a primary antibody to TH (diluted 1 : 20000; mouse monoclonal; Sigma-Aldrich) or 5-HT (1 : 3000; rabbit polyclonal; ImmunoStar, Hudson, WI, U.S.A.) dissolved in PB containing 0.1% Triton X-100, 0.1% sodium azide, and 2% bovine serum albumin. All sections were obtained by the Vector ABC immunohistochemical method (Vectastain Elite kit; Vector, Burlingame, CA, U.S.A.) with diaminobenzidine as the chromogen.…”

Section: Methodsmentioning

confidence: 99%

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Yokukansan, a Traditional Japanese Medicine, Enhances the L-DOPA-Induced Rotational Response in 6-Hydroxydopamine-Lesioned Rats: Possible Inhibition of COMT

Ishida

Ebihara

Tabuchi

et al. 2016

Biological & Pharmaceutical Bulletin

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The aim of the present study was to investigate the effects of the traditional Japanese medicine yokukansan (YKS) on the function of dopamine (DA) in the rat nigrostriatal system. Unilateral 6-hydroxydopamine lesions were produced in the rat nigrostriatal system. Despite a marked loss in the striatal immunoreactivity of tyrosine hydroxylase on the lesion side, striatal serotonin (5-HT) immunoreactivity was not affected. Treatment using L-3,4-dihydroxyphenylalanine (L-DOPA) in conjunction with benserazide for 15 d induced abnormal involuntary movements (AIMs) such as locomotive (rotational response), axial, forelimb, and orolingual movements in the lesioned rats. The L-DOPA-induced locomotive and axial, but not forelimb and orolingual, AIMs were significantly increased and prolonged by the pre-administration of YKS. We next investigated

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“…One popular model in rats is produced by the unilateral stereotaxic injection of the neurotoxin 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle or substantia nigra [4]. In this model, there is an immediate and almost complete destruction of the dopamine neurons of the substantia nigra and of the ventral tegmental area, resulting in near total depletion (2% of normal) of dopamine in the ipsilateral striatum to the 6-OHDA injections [5]. Immunohistochemical study showed there is no evident damage of dopaminergic neurons and fibers in the brain contralateral to the 6-OHDA injections [5,6].…”

Section: -[ 18 F]fluoro-l-34-dihydroxyphenylalanine ([ 18 F]mentioning

confidence: 99%

Changes in Dopamine D2 Receptors and 6-[18F]Fluoro-L-3,4-Dihydroxyphenylalanine Uptake in the Brain of 6-Hydroxydopamine- Lesioned Rats

et al. 2004

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We studied tracer distributions in positron emission tomography of ligands for dopamine D₁ receptors ([¹¹C]SCH23390) and D₂ receptors ([¹¹C]raclopride) and the dopamine precursor analog 6-[¹⁸F]fluoro-L-3,4-dihydroxyphenylalanine ([¹⁸F]FDOPA), as a measurement of presynaptic dopaminergic function, in the brain after 6-hydroxydopamine lesioning of the medial forebrain bundle in rats. The unilateral lesions were confirmed behaviorally by methamphetamine-induced rotation 2 weeks after lesioning, and the brains were analyzed by tissue dissection following an intravenous bolus of each tracer 3 weeks after lesioning. [¹¹C]Raclopride, but not [¹¹C]SCH23390, showed a higher accumulation in the striatum on the lesion side compared with that on the non-lesioned (intact) side. On the other hand, a lower accumulation of [¹⁸F]FDOPA was found in the striatum and cerebral cortex on the lesion side. Our studies demonstrate upregulation of dopamine D₂ receptors in the striatum and a decrease in FDOPA uptake in both the striatum and cerebral cortex ipsilateral to the 6-hydroxydopamine lesions. Therefore, the combination of a D₂ antagonist and FDOPA may provide a potentially useful method for assessing the effects of dopamine depletion in Parkinson’s disease.

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Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis

Cited by 23 publications

References 23 publications

Role of Dopaminergic System in Core Part of Nucleus Accumbens in Hyperlocomotion and Rearing Induced by MK-801 in Rats: A Behavioral and In Vivo Microdialysis Study

Role of Dopaminergic System in Core Part of Nucleus Accumbens in Hyperlocomotion and Rearing Induced by MK-801 in Rats: A Behavioral and In Vivo Microdialysis Study

Yokukansan, a Traditional Japanese Medicine, Enhances the L-DOPA-Induced Rotational Response in 6-Hydroxydopamine-Lesioned Rats: Possible Inhibition of COMT

Changes in Dopamine D<sub>2</sub> Receptors and 6-[<sup>18</sup>F]Fluoro-<i>L</i>-3,4-Dihydroxyphenylalanine Uptake in the Brain of 6-Hydroxydopamine- Lesioned Rats

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