Serotonin uptake by stored platelets

Adams, George A.; Swenson, Sonia D.; Rock, Gail

doi:10.1016/0049-3848(82)90270-5

Cited by 7 publications

(2 citation statements)

References 7 publications

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“…The percentage of release of "C-serotonin was calculated as previously reported. 6 We tested each aggregating agent singly and paired and measured radioactivity release using a liquid scintillation counter (LS7500, Beckman Instruments Inc., Richmond, CA).…”

Section: Platelet Aggregation and Serotonin Releasementioning

confidence: 99%

Storage of platelets in balanced salt solutions: a simple platelet storage medium

Rock¹,

White

Labow

1991

Transfusion

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When a commercially available intravenous solution, plasmalyte-A, was added to platelet pellets as a platelet storage medium (PSM) after more than 85 percent of the plasma had been expressed, the platelets functioned equally well, after 5 days of storage, in platelet aggregation, release reaction, and serotonin uptake as did platelets stored in plasma. The level of fibrinopeptide A was significantly different in the PSM. Similarly, lower levels of kallikrein and complement components in the PSM-stored platelets indicated reduced activation of plasma enzyme systems. Morphology scores showed better shape maintenance in PSM, with values of 255 versus values of 185 in plasma (time zero, 280); electron microscopy also showed somewhat better structural maintenance in PSM. Glucose consumption, lactate and ammonia production, and fatty acid oxidation were also reduced in PSM. No significant differences in pH, white cell count, pO2, pCO2, or lactic dehydrogenase were observed after 5 days of storage in plasma or PSM; however, platelet counts were reduced after 5 days in plasma but remained constant in PSM. The beta-thromboglobulin release was significantly lower in the PSM-stored platelets after 5 days. The volume of platelet membrane glycoprotein lb (which is responsible for platelet adhesion) decreased 15 percent in plasma following storage, whereas a decrease of only 5 percent was noted in PSM. There was less leaching of plasticizers in PSM than in plasma. Survival and recovery using 51Cr-labeled autologous platelets showed that, after 5 days of storage, the recovery of PSM-stored platelets was 63 percent and the survival was 7.7 +/- 0.8 days (multiple hit model).(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Platelet Aggregation and Serotonin Releasementioning

confidence: 99%

Storage of platelets in balanced salt solutions: a simple platelet storage medium

Rock¹,

White

Labow

1991

Transfusion

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“…At the end of 4 min, the suspensions were centrifuged at 15,00 g for 1 min in an Eppendorf centrifuge to obtain supernatant samples. The percent release of 14C-serotonin was cal culated as previously reported [15],…”

Section: Platelet Function Testingmentioning

confidence: 99%

5-Day Storage of Human Platelet Concentrates in 30 ml of Plasma or Artificial Medium

Adams¹,

Swenson²,

Rock³

1987

Vox Sanguinis

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Optimal conditions for the storage of platelet concentrates were studied by changing 5 environmental parameters: bag composition (PL 146 vs. PL732), volume of plasma (60 vs. 30 ml), anticoagulant (CPDA^-1 vs. heparin), nutrient (glucose vs. fructose) and medium (plasma vs. artificial medium). A full bilevel factorial study was conducted to evaluate each variable alone and in combination with the other variables for their effects on platelet aggregation and release in response to single and pairs of stimuli. Serotonin uptake, pCO(2), platelet count, lactate, glucose, pO(2), pH and white blood cell concentration were also measured after 3 and 5 days of storage. Platelets that were stored in PL146 bags had reduced responses to stimulation by 3 days and markedly impaired responses after 5 days relative to platelets that were stored in PL732 bags. There was a large drop in pH and platelet responsiveness when platelets were stored in a volume of 30 ml in PL146 bags; these were not found when platelets were stored in 30 ml in PL732 bags. Replacing plasma with an artificial medium or adding fructose or heparin and calcium to plasma yielded platelets that were equally functional as routine controls in CPD-A1 plasma. It was concluded that replacement of plasma with 60 ml of artificial medium or a reduction of plasma volume with storage in PL732 bags are two possible mechanisms of obtaining more plasma from blood donations without compromising maximum platelet storage life.

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Platelet yield varies but in vitro function is maintained after leukocyte removal using terumo imugard™ filter and cutter Leukotrap™

Gerber

Nydegger

1987

Plasma Therapy and Transfusion Technology

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Serotonin uptake by stored platelets

Cited by 7 publications

References 7 publications

Storage of platelets in balanced salt solutions: a simple platelet storage medium

Storage of platelets in balanced salt solutions: a simple platelet storage medium

5-Day Storage of Human Platelet Concentrates in 30 ml of Plasma or Artificial Medium

Platelet yield varies but in vitro function is maintained after leukocyte removal using terumo imugard™ filter and cutter Leukotrap™

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