Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt

Shabayek, Sarah; Abdalla, Salah; Abou-Zeid, A. M.

doi:10.1017/s0950268813000848

Cited by 34 publications

(30 citation statements)

References 12 publications

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“…In the present study, the rate of GBS among pregnant women was 26.5%, which is in agreement with other Egyptian studies 13,29 and higher than the rates reported in others. 30,31 According to a review article published in 2010, which consists of 76 original articles, the results demonstrated that Middle East are accounted for high risk countries for GBS colonization.…”

Section: Discussionsupporting

confidence: 93%

“…-The mean age of pregnant women carriers for GBS was (25.4 ± 2.9). GBS colonization was common mostly in the age group (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) and this finding was statistically significant (p = 0.01). GBS colonization was statistically significantly higher at 37 weeks of gestation (p = 0.002), among women who had frequent antenatal visits (p=<0.001), and who adopted the condom contraceptive method (p=<0.001).…”

Section: Demographic Data Resultsmentioning

confidence: 90%

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Group B streptococcal carriage, antimicrobial susceptibility, and virulence related genes among pregnant women in Alexandria, Egypt

Sadaka

Aly

Meheissen

et al. 2018

Alexandria Journal of Medicine

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2018) Group B streptococcal carriage, antimicrobial susceptibility, and virulence related genes among pregnant women in Alexandria, a b s t r a c t Background and aim: Group B Streptococcus (GBS) has emerged as a leading cause of illness and death among neonates. The study was conducted to estimate the prevalence of recto-vaginal carriage of GBS among pregnant women at 35-37 weeks, gestation, to describe GBS antimicrobial susceptibility profile and to investigate selected virulence genes by PCR. Subjects and methods: Two-hundred pregnant women at 35-37 weeks of gestation attending antenatal clinic at Al-Shatby University Hospital were enrolled in the study. Both vaginal and rectal swabs were collected from each subject. Swabs were inoculated onto CHROMagar TM StrepB and sheep blood agar plates. All GBS isolates were subjected to antimicrobial susceptibility testing using disc diffusion. Disc approximation test was performed to detect erythromycin resistance phenotype (MLSB). GBS virulence genes scpB, bac, bca, and rib were identified by PCR. Results: Among the 200 pregnant women, 53 (26.5%) were identified as GBS carriers. All carriers had vaginal colonization (100%), four (7.5%) had combined recto-vaginal colonization. None of the carriers had rectal colonization alone. All isolates (100%) were susceptible to penicillin, ampicillin, ceftriaxone, cefotaxime, cefepime, vancomycin, and linezolid. On the other hand, 43.4%, 28.3%, 22.6%, and 15% of isolates were resistant to levofloxacin, azithromycin, erythromycin, and clindamycin respectively. Out of 12 erythromycin resistant isolates, six isolates had constitutive while two had inducible MLSB resistance. scpB was identified in 100%, rib in 79.2%, and bac in 35.8% of GBS isolates. None of the isolates possessed the bca gene. Conclusion: Introduction of GBS screening in Egyptian pregnant women is recommended. Penicillin or ampicillin is still the antibiotic of choice for intrapartum prophylaxis. Ó 2017 Alexandria University Faculty of Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Section: Discussionsupporting

confidence: 93%

Section: Demographic Data Resultsmentioning

confidence: 90%

Group B streptococcal carriage, antimicrobial susceptibility, and virulence related genes among pregnant women in Alexandria, Egypt

Sadaka

Aly

Meheissen

et al. 2018

Alexandria Journal of Medicine

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“…Epidemiological distribution of these 10 serotypes can vary depending on geographical region, population under study and the source of the bacterial isolate 48. Studies from Gambia49 and Egypt50 have reported serotype V as the most common serotype, while in South Africa and Morocco13 51 52 serotypes Ia and III are dominant with a range of 30%–40% and serotype V only with 10% of isolates.…”

Section: Resultsmentioning

confidence: 99%

Incidence and serotype characterisation ofStreptococcus agalactiaein a Portuguese hospital

et al. 2017

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“…It is reported that S. agalactiae strains usually carry at least one of the alpha-like proteins, of which proteins Epsilon (Alp1), Alp2, Alp3, Alp4, Alpha-C and Rib have been extensively studied [17], although several bovine and human strains have been reported to be negative for any of the six proteins [16,17,18]. In this study, the multiple PCR assay for detecting Alp genes showed that 92% of the tested bovine isolates were non-typeable, possibly due to mismatch of the primers for genes encoding alpha-like proteins in these isolates.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Streptococcus agalactiae Isolated from Bovine Mastitis in Eastern China

et al. 2013

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One hundred and two Streptococcus agalactiae (group B streptococcus [GBS]) isolates were collected from dairy cattle with subclinical mastitis in Eastern China during 2011. Clonal groups were established by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE), respectively. Capsular polysaccharides (CPS), pilus and alpha-like-protein (Alp) family genes were also characterized by molecular techniques. MLST analysis revealed that these isolates were limited to three clonal groups and were clustered in six different lineages, i.e. ST (sequence type) 103, ST568, ST67, ST301, ST313 and ST570, of which ST568 and ST570 were new genotypes. PFGE analysis revealed this isolates were clustered in 27 PFGE types, of which, types 7, 8, 14, 15, 16, 18, 23 and 25 were the eight major types, comprising close to 70% (71/102) of all the isolates. The most prevalent sequence types were ST103 (58% isolates) and ST568 (31% isolates), comprising capsular genotype Ia isolates without any of the detected Alp genes, suggesting the appearance of novel genomic backgrounds of prevalent strains of bovine S. agalactiae. All the strains possessed the pilus island 2b (PI-2b) gene and the prevalent capsular genotypes were types Ia (89% isolates) and II (11% isolates), the conserved pilus type providing suitable data for the development of vaccines against mastitis caused by S. agalactiae.

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Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt

Cited by 34 publications

References 12 publications

Group B streptococcal carriage, antimicrobial susceptibility, and virulence related genes among pregnant women in Alexandria, Egypt

Group B streptococcal carriage, antimicrobial susceptibility, and virulence related genes among pregnant women in Alexandria, Egypt

Incidence and serotype characterisation ofStreptococcus agalactiaein a Portuguese hospital

Molecular Characterization of Streptococcus agalactiae Isolated from Bovine Mastitis in Eastern China

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