Serotype specific polymerase chain reaction identifies a higher prevalence of streptococcus mutans serotype k and e in a random group of children with dental caries from the Southern region of India

Rao, Arun; Austin, Ravi David

doi:10.4103/0976-237x.137905

Cited by 10 publications

(20 citation statements)

References 29 publications

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“…The prevalence of serotype k (17.5%) reported here is higher than previously reported for Japanese (1.4–2%), Finnish (3.6%), and Thai (2–2.8%) subjects . A study of subjects from southern India reported a higher prevalence (26%) of serotype k than in those studies. However, in contrast to the Indian study , which used saliva, the present study was conducted using individual S. mutans isolates.…”

Section: Discussioncontrasting

confidence: 81%

“…A study of subjects from southern India reported a higher prevalence (26%) of serotype k than in those studies. However, in contrast to the Indian study , which used saliva, the present study was conducted using individual S. mutans isolates. It is possible that analysis of saliva samples in the Uniontown population would result in a higher prevalence of serotype k , comparable to that of the Indian study.…”

Section: Discussionmentioning

confidence: 60%

“…, in 2004, which is now commonly used in serotyping by multiplex PCR. A recent study in India of whole salvia reported 23% of isolates as undetermined serotypes; however, this study did not include serotype c in the analysis . In 2007, Nakano et al .…”

Section: Discussionmentioning

confidence: 95%

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Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing

Momeni

Whiddon

Cheon

et al. 2015

European J Oral Sciences

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Studies using multilocus sequence typing (MLST) have demonstrated that Streptococcus mutans isolates are genetically diverse. Our laboratory previously demonstrated clonality of S. mutans using MLST but could not discount the possibility of sampling bias. In this study, the clonality of randomly selected S. mutans plaque isolates from African American children was examined using MLST. Serotype and presence of collagen-binding proteins (CBP) cnm/cbm were also assessed. One hundred S. mutans isolates were randomly selected for MLST analysis. Sequence analysis was performed and phylogenetic trees were generated using START2 and MEGA. Thirty-four sequence types (ST) were identified of which 27 were unique to this population. Seventy-five percent of the isolates clustered into 16 clonal groups. Serotypes observed were c (n=84), e (n=3), and k (n=11). The prevalence of S. mutans isolates serotype k was notably high at 17.5%. All isolates were cnm/cbm negative. The clonality of S. mutans demonstrated in this study illustrates the importance of localized populations studies and are consistent with transmission. The prevalence of serotype k, a recently proposed systemic pathogen, observed in this study is higher than reported in most populations and is the first report of S. mutans serotype k in a US population.

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 60%

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Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing

Momeni

Whiddon

Cheon

et al. 2015

European J Oral Sciences

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“…All four serotypes were originally described using immunodiffusion method . Studies have already summarized the prevalence of S mutans serotypes by this approach and other traditional approaches . More recently, PCR‐based identification approaches have been developed and utilized for serotyping based on the variability in genes associated with the RGP synthesis.…”

Section: Introductionmentioning

confidence: 99%

Streptococcus mutans serotypes and collagen‐binding proteins Cnm/Cbm in children with caries analysed by PCR

Momeni

Ghazal

Grenett

et al. 2019

Molecular Oral Microbiology

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Streptococcus mutans, a primary bacterium associated with dental caries, has four known clinical serotypes (c, e, f and k). Certain serotypes, the presence of multiple serotypes and strains with collagen-binding proteins (CBP, Cnm and Cbm) have been linked with systemic disease. Evaluation of S mutans serotype distribution and caries association is needed in the United States. The purpose of this study was to evaluate the prevalence of S mutans serotypes from two cohorts of African-American children in rural Alabama using three sample types (saliva, plaque and individual S mutans isolates) by PCR detection for association with caries. Detection of CBP was also performed by PCR. In total, 129 children were evaluated and overall prevalence of serotypes were: serotype c(98%), e(26%), f(7%) and k(52%). Serotype c was statistically associated with higher caries scores in older children (P < 0.001) and serotype k was statistically more likely in females (P = 0.004). Fourteen per cent of children had CBP. Thirteen S mutans isolates from five children tested positive for both CBP. This study is the first to report on the prevalence of S mutans serotypes in a US population using the PCR-based approach. The frequency of serotype k in this study is the highest reported in any population, illustrating the need for further study to determine the prevalence of this clinically relevant serotype in the US. This is the first study to report S mutans isolates with both Cnm and Cbm in the same strain, and further analysis is needed to determine the clinical significance of these strains. K E Y W O R D Scollagen-binding proteins, dental caries, PCR, serotypes, Streptococcus mutans | 65 MOMENI Et al.

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“…[ 2 ] Plaque formation per se is conceived as a physiologically normal event, which otherwise is disrupted and removed by effective brushing techniques. However, when maintenance of oral hygiene suffers the bacterial flora within the plaque begins to mature with early and late colonizers,[ 3 4 ] which in the availability of carbohydrate rich substrates becomes preferentially populated by acidogenic and acid-tolerant species. [ 5 6 ]…”

Section: Introductionmentioning

confidence: 99%

16S rRNA gene-based metagenomic analysis identifies a novel bacterial co-prevalence pattern in dental caries

et al. 2015

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Objective:To identify the prevalence of acidogenic and nonacidogenic bacteria in patients with polycaries lesions, and to ascertain caries specific bacterial prevalence in relation to noncaries controls.Materials and Methods:Total genomic DNA extracted from saliva of three adults and four children from the same family were subjected to 16S rRNA gene sequencing analysis on a next generation sequencer, the PGS-Ion Torrent. Those bacterial genera with read counts > 1000 were considered as significant in each of the subject and used to associate the occurrence with caries.Results and Conclusion:Sequencing analysis indicated a higher prevalence of Streptococcus, Rothia, Granulicatella, Gemella, Actinomyces, Selenomonas, Haemophilus and Veillonella in the caries group relative to controls. While higher prevalence of Streptococcus, Rothia and Granulicatella were observed in all caries samples, the prevalence of others was observable in 29–57% of samples. Interestingly, Rothia and Selenomonas, which are known to occur within anaerobic environments of dentinal caries and subgingival plaque biofilms, were seen in the saliva of these caries patients. Taken together, the study has identified for the first time a unique co-prevalence pattern of bacteria in caries patients that may be explored as distinct caries specific bacterial signature to predict cariogenesis in high-risk primary and mixed dentition age groups.

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Serotype specific polymerase chain reaction identifies a higher prevalence of streptococcus mutans serotype k and e in a random group of children with dental caries from the Southern region of India

Cited by 10 publications

References 29 publications

Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing

Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing

Streptococcus mutans serotypes and collagen‐binding proteins Cnm/Cbm in children with caries analysed by PCR

16S rRNA gene-based metagenomic analysis identifies a novel bacterial co-prevalence pattern in dental caries

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