Serotypes, Pathogenic Potential, and Antimicrobial Resistance of &lt;i&gt;Escherichia coli&lt;/i&gt; Isolated from Subclinical Bovine Mastitis Milk Samples in Egypt

Ombarak, Rabee; Zayda, Mahmoud Gamaleldin; Awasthi, Sharda Prasad; Hinenoya, Atsushi; Yamasaki, Shinji

doi:10.7883/yoken.jjid.2018.538

Cited by 19 publications

(11 citation statements)

References 11 publications

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“…Absence of stx genes in the E. coli is in agreement with the findings reported earlier China, Iran and Belgium ( Lan et al, 2020 , Mansouri-Najand and Khalili, 2007 , Vivegnis et al, 1999 ). However, occurrence of stx genes in E. coli have also been reported from bovine clinical and subclinical mastitis ( Claeys et al, 2013 , Jayarao et al, 2006 , Little et al, 2008 , Momtaz et al, 2012 , Ombarak et al, 2019 , Pradel et al, 2008 , Van Kessel et al, 2011 ). In addition to stx , occurrence of eae in E. coli isolated from bovine mastitis milk was also reported by several authors ( Lan et al, 2020 , Momtaz et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Virulence determinants and antimicrobial resistance of E. coli isolated from bovine clinical mastitis in some selected dairy farms of Bangladesh

Bag

Khan

Sami

et al. 2021

Saudi Journal of Biological Sciences

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E. coli is one of the major significant pathogens causing mastitis, the most complex and costly diseases in the dairy industry worldwide. Present study was undertaken to isolate, detect the virulence factors, phylogroup, antimicrobial susceptibility and antimicrobial resistance genes in E. coli from cows with clinical mastitis. A total of 68 milk samples comprising 53 from clinical mastitis and 15 from apparently healthy cattle were collected from four different established dairy farms in Bangladesh. E. coli was isolated from the milk samples and identified by PCR targeting malB gene and sequencing of 16S rRNA gene. E. coli isolates were screened by PCR for the detection of major virulence genes ( stx , eae and cdt ) of diarrheagenic E. coli followed by phylogenetic grouping. Antimicrobial susceptibility of the E. coli isolates was determined by disk diffusion test and E. coli showing resistance was further screened for the presence of antimicrobial resistance genes. E. coli was isolated from 35.8% of the mastitis milk samples but none from the apparently healthy cattle milk. All the E. coli isolates were negative for stx , eae and cdt genes and belonged to the phylogenetic groups A and B1 which comprising of commensal E. coli . Antibiotic sensitivity testing revealed 84.2% (16/19) of the isolates as multidrug resistant. Highest resistance was observed against amoxicillin (94.5%) followed by ampicillin (89.5%) and tetracycline (89.5%). E. coli were found resistant against all the classes of antimicrobials used at the farm level. Tetracycline resistance gene ( tetA ) was detected in 100% of the tetracycline resistant E. coli and bla TEM-1 was present in 38.9% of the E. coli isolates. Findings of this study indicate a potential threat of developing antimicrobial resistance in commensal E. coli and their association with clinical mastitis. Occurrence of multidrug resistant E. coli might be responsible for the failure of antibiotic therapies in clinical mastitis as well as pose potential threat of transmitting and development of antibiotic resistance in human.

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Section: Discussionmentioning

confidence: 99%

Virulence determinants and antimicrobial resistance of E. coli isolated from bovine clinical mastitis in some selected dairy farms of Bangladesh

Bag

Khan

Sami

et al. 2021

Saudi Journal of Biological Sciences

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“…In the study, the mannose-specific adhesion type 1 fimbriae gene, fimH , among eight virulence profiles ( eaeA , stx1 , stx2 , hly , fimH , iucC , fyuA , and iutA genes), was the most prevalent virulence gene (73.2%). Other studies also reported that fimH was the most widespread virulence gene of E. coli isolates from the subclinical mastitis milk samples (93%) in Egypt [ 24 ] and clinical mastitis milk samples (84.2%) in Taiwan [ 25 ]. The fimH gene was associated with the initial colonization of tissues, and uropathogenic E. coli strains were reported to express genes by more than 90% [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Other studies also reported that fimH was the most widespread virulence gene of E. coli isolates from the subclinical mastitis milk samples (93%) in Egypt [ 24 ] and clinical mastitis milk samples (84.2%) in Taiwan [ 25 ]. The fimH gene was associated with the initial colonization of tissues, and uropathogenic E. coli strains were reported to express genes by more than 90% [ 24 ]. In contrast, only one isolate (4.3%) from phylogenetic group A, considered commensal, carried the fimH gene in this study.…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of Escherichia coli from bulk tank milk in Korea

Yoon

Lee

2022

J Vet Sci

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Background Escherichia coli , which causes subclinical or clinical mastitis in cattle, is responsible for transmitting antimicrobial resistance via human consumption of raw milk or raw milk products. Objectives The objective of this study was to investigate the molecular characteristics of 183 E. coli from bulk tank milk of five different dairy factories in Korea. Methods The molecular characteristics of E. coli such as serogroup, virulence, antimicrobial resistance, and integron genes were detected using polymerase chain reaction and antimicrobial susceptibility were tested using the disk diffusion test. Results In the distribution of phylogenetic groups, group D was the most prevalent (59.6%) and followed by group B1 (25.1%). The most predominant serogroup was O173 (15.3%), and a total of 46 different serotypes were detected. The virulence gene found most often was fimH (73.2%), and stx1 , fimH , incC , fyuA , and iutA genes were significantly higher in isolates of phylogenetic group B1 compared to phylogenetic groups A, B2, and D ( p < 0.05). Among 64 E. coli isolates that showed resistance to at least one antimicrobial, the highest resistance rate was observed for tetracyclines (37.5%). All 18 integron-positive E. coli carried the integron class I ( int1 ) gene, and three different gene cassette arrangements, dfrA12 + aadA2 (2 isolates), aac(6′)-Ib3+aac(6′)-Ib-cr+aadA4 (2 isolates), and dfrA17+aadA5 (1 isolate) were detected. Conclusions These data suggest that the E. coli from bulk tank milk can be an indicator for dissemination of antimicrobial resistance and virulence factors via cross-contamination.

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“…[56][57][58][59][60] Some recent studies support this view, and populationbased reports revealed that the use of ACEI and ARB inhibitors in patients with COVID-19 does not aggravate the severity of the disease, increase the risk of infection, improve prognosis, or reduce mortality. [61][62][63][64][65][66] In addition, a systematic review and meta-analysis of related research has reconfirmed this thesis. [67][68][69][70][71] Given that hypertension and CVD can increase the severity of the disease, dividing patients without hypertensive and CVD into a non-ACEI/ARB group may underestimate the favorable effects of ACEI/ARB drugs on affected patients.…”

Section: Sars-cov-2mentioning

confidence: 60%

<p>Role of Renin-Angiotensin System in Acute Lung Injury Caused by Viral Infection</p>

Gao¹,

Du²,

Zhang³

et al. 2020

IDR

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The renin-angiotensin system (RAS) is the most important regulatory system of electrolyte homeostasis and blood pressure and acts through angiotensin-converting enzyme (ACE)/angiotensin II (Ang II)/Ang II type 1 (AT1) receptor axis and angiotensin-converting enzyme 2 (ACE2)/angiotensin (1-7)/MAS receptor axis. RAS dysfunction is related to the occurrence and development of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) and causes a serious prognosis and even death. ALI/ARDS can be induced by various ways, one of which is viral infections, such as SARS-CoV, SARS-CoV-2, H5N1, H7N9, and EV71. This article reviews the specific mechanism on how RAS dysfunction affects ALI/ARDs caused by viral infections. SARS-CoV and SARS-CoV-2 enter the host cells by binding with ACE2. H5N1 and H7N9 avian influenza viruses reduce the ACE2 level in the body, and EV71 increases Ang II concentration. Treatment with angiotensin-converting enzyme inhibitor and angiotensin AT1 receptor blocker can alleviate ALI/ARDS symptoms. This review provides suggestions for the treatment of lung injury caused by viral infections.

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Serotypes, Pathogenic Potential, and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Subclinical Bovine Mastitis Milk Samples in Egypt

Cited by 19 publications

References 11 publications

Virulence determinants and antimicrobial resistance of E. coli isolated from bovine clinical mastitis in some selected dairy farms of Bangladesh

Virulence determinants and antimicrobial resistance of E. coli isolated from bovine clinical mastitis in some selected dairy farms of Bangladesh

Molecular characteristics of Escherichia coli from bulk tank milk in Korea

<p>Role of Renin-Angiotensin System in Acute Lung Injury Caused by Viral Infection</p>

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