SERPINE1 and its co-expressed genes are associated with the progression of clear cell renal cell carcinoma

Abstract

Background Clear cell renal cell carcinoma(ccRCC) is a frequently occurring malignant tumor of the urinary system. Despite extensive research, the regulatory mechanisms underlying the pathogenesis and progression of ccRCC remain largely unknown. Methods We downloaded 5 ccRCC expression profiles from the Gene Expression Omnibus (GEO) database and obtained the list of differentially expressed genes (DEGs). Using String and Cytoscape tools, we determined the hub genes of ccRCC, and then analyzed their relationship with ccRCC patient survival. Ultimately, we identified SERPINE1 as a prognostic factor in ccRCC. Meanwhile, we confirmed the role of SERPINE1 in 786-O cells by cell transfection and in vitro experiments. Results Our analysis yielded a total of 258 differentially expressed genes, comprising 105 down-regulated genes and 153 up-regulated genes. Survival analysis of SERPINE1 expression in The Cancer Genome Atlas (TCGA) confirmed its association with the increase of tumor grade, lymph node metastasis, and tumor stage, as well as with shorter survival. Furthermore, we found that SERPINE1 expression levels were associated with CD8 + T cells, CD4 + T cells, B cells, macrophages, neutrophils, and dendritic cells. Cell experiments showed that knockdown SERPINE1 expression could inhibit the proliferation, migration and invasion of ccRCC cells. Among the co-expressed genes with the highest correlation, ITGA5, SLC2A3, SLC2A14, SHC1, CEBPB, and ADA were overexpressed and associated with shorter overall survival (OS) in ccRCC. Conclusions In this study, we identified hub genes that are strongly related to ccRCC, and highlights the potential utility of overexpressed SERPINE1 and its co-expressed genes could be used as prognostic and diagnostic biomarkers in ccRCC.

Integrated bioinformatics analysis of noncoding RNAs with tumor immune microenvironment in gastric cancer

“…Moreover, SERPINE1 was associated with immune cells in the TIME of stomach adenocarcinoma 65 . Another study discovered that SERPINE1 expression was correlated with several types of immune cells, such as neutrophils, macrophages, CD4+ T cells, CD8+ T cells, B cells, and dendritic cells in clear cell renal cell carcinoma 66 .…”

Examining Transcriptomic Alterations in Rat Models of Intracerebral Hemorrhage and Severe Intracerebral Hemorrhage

“…Key genes identified from the plots were annotated with their respective fold change and p -value, highlighting those with the most pronounced expression differences. For instance, genes such as Timpg1 and Serpine1 appear prominently in the ICH versus normal comparison, suggesting their potential roles in the pathophysiological response to ICH [ 53 , 54 ].…”

Assessment of the progression of kidney renal clear cell carcinoma using transcriptional profiles revealed new cancer subtypes with variable prognosis

“…While apelin signaling has also been linked to the development of cancer and its progression (Liu et al, 2021). It is thus noteworthy, that the survival analysis of SERPINE1 expression in TCGA found a correlation between shorter survival, and the increased tumor grade, lymph node metastasis, and tumor stage (Guo et al, 2023). Therefore, SERPINE1 plays a crucial role in the progression of KIRC.…”