SERS-active Au@Ag nanorod dimers for ultrasensitive dopamine detection

Tang, Li‐Juan; Li, Si; Han, Fei; Liu, Liqiang; Xu, Liguang; Ma, Wei; Kuang, Hua; Li, Aike; Wang, Libing

doi:10.1016/j.bios.2015.04.013

Cited by 191 publications

(88 citation statements)

References 33 publications

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“…To further confirm the specificity of this method, these five interfering chemicals were mixed as the substrate for DA detection. 18 The results revealed that the interfering chemicals had no influence on DA detection. Therefore, this method has an excellent selectivity for the detection of DA.…”

Section: Selectivity For Da Detectionmentioning

confidence: 95%

“…The recent development of nanotechnology has made it possible to fabricate metal nanostructures with a highly controlled morphology. 18 By introducing abundant nanogaps, nanobridges, sharp protrusions, or crevices into the nanostructures, SERS signals with high EFs of 10 7 -10 14 , as well as improved uniformity and reproducibility, have been obtained. 17,19 In recent years, various nanostructures have been used for SERS detection in DA molecules.…”

Section: Introductionmentioning

confidence: 99%

“…20 An LOD of 1.05 pM DA level has been obtained by using an Ag/MIL-101 nanostructure, 11 an LOD of 0.1 mM by using flower-like gold nanostructures electrodeposited on indium tin oxide glass, 13,20 an LOD of 1 nM with graphene-Au nanopyramid heterostructure, 13 and an LOD of 0.006 pM has been achieved with Au@Ag nanorod dimers. 18 Meanwhile, efforts have been made for the quantitative analysis of DA. For example, Ag-Fe 3 O 4 nanocomposites possessing an internal reference were fabricated and used to quantitatively detect DA, and the LOD reached 10 μM.…”

Section: Introductionmentioning

confidence: 99%

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Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers

Yu¹,

He²,

Yang³

et al. 2018

IJN

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BackgroundDopamine (DA) is an important neurotransmitter in the hypothalamus and pituitary gland, which can produce a direct influence on mammals’ emotions in midbrain. Additionally, the level of DA is highly related with some important neurologic diseases such as schizophrenia, Parkinson, and Huntington’s diseases, etc. In light of the important roles that DA plays in the disease modulation, it is of considerable significance to develop a sensitive and reproducible approach for monitoring DA.PurposeThe objective of this study was to develop an efficient approach to quantitatively monitor the level of DA using Ag nanoparticle (NP) dimers and enhanced Raman spectroscopy.MethodsAg NP dimers were synthesized for the sensitive detection of DA via surface-enhanced Raman scattering (SERS). Citrate was used as both the capping agent of NPs and sensing agent to DA, which is self-assembled on the surface of Ag NP dimers by reacting with the surface carboxyl group to form a stable amide bond. To improve accuracy and precision, the multiplicative effects model for surface-enhanced Raman spectroscopy was utilized to analyze the SERS assays.ResultsA low limits of detection (LOD) of 20 pM and a wide linear response range from 30 pM to 300 nM were obtained for DA quantitative detection. The SERS enhancement factor was theoretically valued at approximately 107 by discrete dipole approximation. DA was self-assembled on the citrate capped surface of Ag NPs dimers through the amide bond. The adsorption energy was estimated to be 256 KJ/mol using the Langmuir isotherm model. The density functional theory was used to simulate the spectral characteristics of SERS during the adsorption of DA on the surface of the Ag dimers. Furthermore, to improve the accuracy and precision of quantitative analysis of SERS assays with a multiplicative effects model for surface-enhanced Raman spectroscopy.ConclusionA LOD of 20 pM DA-level was obtained, and the linear response ranged from 30 pM to 300 nM for quantitative DA detection. The absolute relative percentage error was 4.22% between the real and predicted DA concentrations. This detection scheme is expected to have good applications in the prevention and diagnosis of certain diseases caused by disorders in the DA level.

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Section: Selectivity For Da Detectionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers

Yu¹,

He²,

Yang³

et al. 2018

IJN

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“…The 60 nm diameter AgNPs were synthesized by the published method 35, 36. Briefly, AgNO 3 (90 mg) was dissolved in 500 mL of H 2 O and brought to boiling.…”

Section: Methodsmentioning

confidence: 99%

Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay

Zhao

Han

et al. 2017

Theranostics

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Detection of biomarkers is extremely important for the early diagnosis of diseases. Here, we developed an easy and highly sensitive fluorescence detection system for the determination of biomarkers by combining the rapid separation of magnetic beads and silver nanoparticles labeled antibodies. An ultrasensitive silver ions fluorescence probe 3', 6'-bis (diethylamino)-2-(2-iodoethyl) spiro[isoindoline-1, 9'-xanthen]-3-one (Ag+-FP) was applied to immunoassay. A significant signal amplification was achieved as the AgNPs can be dissolved by H2O2 and generate numerous Ag+, which would turn “on” the fluorescence of Ag+-FP. Using α-fetoprotein (AFP) and C-reactive protein (CRP) as target analytes, good linear responses were obtained from 0.1 to 10 ng mL-1 and the limits of detection (LOD) were as low as 70 pg·mL-1 and 30 pg·mL-1, respectively. In addition, the developed system was further evaluated for the detection of real samples including 30 positive serum specimens obtained from hepatocarcinoma patients and 20 negative serum samples, and performs as well as the commercial electrochemiluminescence immunoassay (ECLI) method with less cost and more convenience. Thus, the designed detection system can be used as a promising platform for the detection of a variety of biomarkers and served as a powerful tool in clinical diagnosis.

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“…S2 †), which is a powerful tool to measure particle size distribution. 36,[38][39][40] The average hydrodynamic diameter of the AuNRs shows little change aer the surfactant exchange process. However, an average increase of about 25 nm was observed aer the Ab/DNA complex was conjugated onto AuNRs, also indicating the successful conjugation process.…”

Section: -37mentioning

confidence: 99%

Digital immunoassay of a prostate-specific antigen using gold nanorods and magnetic nanoparticles

Zhu

Sun

et al. 2017

RSC Adv.

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Herein, a digital immunoassay was developed by counting highly confined gold nanorod (AuNR) probes with the aid of magnetic nanoparticles (MNPs). MNPs were conjugated with capture antibodies to concentrate the target analytes. AuNRs were conjugated with a mixture of the detection antibody and randomly selected DNA, where the detection antibody was applied to signal the target binding event and DNA was applied to provide sufficient negative charges. The bicomponent AuNR probes in the targetmediated immunocomplexes were eluted and confined onto a positively charged glass slide on the basis of electrostatic attraction for accurate counting. The combination of the magnetic concentration of the target analytes and the confinement of the DNA/antibody AuNR probes results in improved detection efficiency; a good linear range from 0.01 to 10 pg mL À1 and a detection limit of 8 fg mL À1 ($226 aM)were achieved for the detection of prostate-specific antigen (PSA). The proposed method offers the simultaneous advantages of high sensitivity, digital quantification accuracy, lack of requirement for signal or target amplification, wide dose response curve and low detection cost; thus, it shows good potential in practical applications.

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SERS-active Au@Ag nanorod dimers for ultrasensitive dopamine detection

Cited by 191 publications

References 33 publications

Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers

Sensitive determination of dopamine levels via surface-enhanced Raman scattering of Ag nanoparticle dimers

Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay

Digital immunoassay of a prostate-specific antigen using gold nanorods and magnetic nanoparticles

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