Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Reis, Mariana M. S.; Moreira, Ana C.; Sousa, Mário; Mathur, Premendu P.; Oliveira, Pedro F.; Alves, Marco G.

doi:10.1002/jat.3122

Cited by 73 publications

(39 citation statements)

References 156 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their functions could be altered through a mechanism mediated by oxidative stress and mitochondrial dysfunction caused by endocrine disruptor (Reis et al, 2015). Thus, the results above indicated that the oral administration of TPP and TCEP for 35 days had adverse effects on the endocrine system in male mice.…”

Section: Discussionmentioning

confidence: 87%

Exposure of male mice to two kinds of organophosphate flame retardants (OPFRs) induced oxidative stress and endocrine disruption

Chen

Jin

et al. 2015

Environmental Toxicology and Pharmacology

134

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 87%

Exposure of male mice to two kinds of organophosphate flame retardants (OPFRs) induced oxidative stress and endocrine disruption

Chen

Jin

et al. 2015

Environmental Toxicology and Pharmacology

134

View full text Add to dashboard Cite

“…This in vitro model has also been used for mechanistic studies to probe the molecular events underlying toxicant-induced Sertoli cell TJ-barrier disruption or Sertoli cell-gonocyte interactions, such as cadmium, BPA, PFOS and phthalates, which faithfully reproduce findings in vivo such as by identifying the downstream signaling MAPKs p38, ERK1/2 or JNK (Table 1) . Thus, Sertoli cells cultured in vitro is a good and reliable model to study male reproductive toxicology [58]. It must be noted that findings based on this in vitro system using rat (or mouse) Sertoli cells may not be applicable to reproductive toxicology in humans in vivo for a number of reasons.…”

Section: Rat Versus Human Sertoli Cell In Vitro Models To Study Envirmentioning

confidence: 99%

Is toxicant-induced Sertoli cell injury in vitro a useful model to study molecular mechanisms in spermatogenesis?

Mruk

Lee

et al. 2016

Seminars in Cell & Developmental Biology

View full text Add to dashboard Cite

Sertoli cells isolated from rodents or humans and cultured in vitro are known to establish a functional tight junction (TJ)-permeability barrier that mimics the blood-testis barrier (BTB) in vivo. This model has been widely used by investigators to study the biology of the TJ and the BTB. Studies have shown that environmental toxicants (e.g., perfluorooctanesulfonate (PFOS), bisphenol A (BPA) and cadmium) that exert their disruptive effects to induce Sertoli cell injury using this in vitro model are reproducible in studies in vivo. Thus, this in vitro system provides a convenient approach to probe the molecular mechanism(s) underlying toxicant-induced testis injury but also to provide new insights in understanding spermatogenesis, such as the biology of cell adhesion, spermatid transport, and others. Herein, we provide a brief and critical review based on studies using this in vitro model of Sertoli cell cultures using primary cells isolated from rodent testes versus humans to monitor environmental toxicant-mediated Sertoli cell injury, and this information is relevant to the molecular mechanisms that regulate spermatogenesis. In short, recent findings have shown that environmental toxicants exert their effects on Sertoli cells to induce testis injury through their action on Sertoli cell actin- and/or microtubule-based cytoskeleton. These effects are mediated via their disruptive effects on actin- and/or microtubule-binding proteins. Sertoli cells also utilize differential spatiotemporal expression of these actin binding proteins to confer plasticity to the BTB to regulate germ cell transport across the BTB.

show abstract

“…It is now well established that the in vitro study of SC physiology is a useful model to disclose deleterious effects of toxicant exposure to male fertility and to probe the molecular mechanisms that regulate spermatogenesis (Li et al 2016;Reis et al 2015). Therefore, in the present study, we established both the SC and rat models to determine the effect of NP on the activity of autophagy in SCs and to explore its underlying mechanisms in view of the intriguing role of AMPK-mTOR signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells

Duan

Quan

et al. 2017

Toxicology Letters

View full text Add to dashboard Cite

The estrogenic chemical 4-nonylphenol (NP) is known to impair testicular devolopment and spermatogenesis in rodents. The objective of this study was to explore the effects of NP on autophagy induction and AMPK-mTOR signaling pathway in Sertoli cells (SCs), which are the "nursemaid cells" for meiosis of spermatocytes. In this study we exposed 7-week-old male rats to NP by intra-peritoneal injection at 0, 20, 50 or 100 mg/kg body weight/2 days for 20 consecutive days. Our results showed that exposure to NP dose-dependently induces the formation of autophagosomes in SCs, increases the expression of Beclin-1, the conversion of LC3-I to LC3-II and the mRNA expression of Atg3, Atg5, Atg7 and Atg12 in testis, and these effects are concomitant with the activation of AMPK, and the suppression of TSC2-mTOR-p70S6K/4EBP1 signaling cascade in testis. Furthermore, 10 µM Compound C or AMPKα1 siRNA pre-treatment effectively attenuated autophagy and reversed AMPK-mTOR-p70S6K/4EBP1 signaling in NP-treated SCs. Co-treatment with 1 mM AICAR remarkably strengthened NP-induced autophagy and mTOR inhibition in SCs. Together, these data suggest that NP stimulates Sertoli cell autophagy and inhibits mTOR-p70S6K/4EBP1 activity through AMPK activation, which is the potential mechanism responsible for the regulation of testis function and differentiation following NP exposure.

show abstract

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Cited by 73 publications

References 156 publications

Exposure of male mice to two kinds of organophosphate flame retardants (OPFRs) induced oxidative stress and endocrine disruption

Exposure of male mice to two kinds of organophosphate flame retardants (OPFRs) induced oxidative stress and endocrine disruption

Is toxicant-induced Sertoli cell injury in vitro a useful model to study molecular mechanisms in spermatogenesis?

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells

Contact Info

Product

Resources

About