Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells

Geens, Mieke; Sermon, Karen; Velde, H. Van de; Tournaye, Herman

doi:10.1007/s10815-011-9541-9

Cited by 27 publications

(34 citation statements)

References 34 publications

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“…; Geens et al . ). In contrast, meiotic‐specific marker expression was not influence by the treatment of monkey ES cell‐derived EBs with the same concentration of BMP4 (Yamauchi et al .…”

Section: Discussionmentioning

confidence: 97%

Effect of BMP4 preceded by retinoic acid and co‐culturing ovarian somatic cells on differentiation of mouse embryonic stem cells into oocyte‐like cells

et al. 2015

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Bone morphogenetic protein 4 (BMP4) and retinoic acid (RA) signaling are the key regulators for germ cell and meiosis induction, respectively. Gonadal tissue also provides an appropriate microenvironment for oocyte differentiation in vivo. The current study aimed to determine whether mimicking in vivo niche is more efficient for oocyte differentiation from embryonic stem (ES) cells. Here, differentiation of mouse ES cells toward oocyte-like cells using embryoid body (EB) and monolayer protocols was induced in the presence (+BMP4) or absence (-BMP4) of BMP4. On day 5, each group was co-cultured with ovarian somatic cells in the presence or absence of RA (+RA or -RA) for an additional 14 days. Our results showed a significant increase in expression of meiotic markers in the +BMP4 condition in EB differentiation protocol. Further differentiation with ovarian somatic cells led to a subpopulation of oocyte-like cell formation. Compared to the controls, the +RA condition resulted in a significant elevation of the meiotic gene expression in contrast to Oct4 that significantly decreased in both protocols. In the cells pre-treated with BMP4 and then exposed to RA in the monolayer differentiation protocol, the gene expression levels of germ cell, Mvh, and maturation markers, Cx37, Zp2, and Gdf9, were also upregulated significantly. Therefore, it can be concluded that +BMP4 and +RA along with ovarian somatic cell co-culture improved the rate of in vitro oocyte differentiation.

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Section: Discussionmentioning

confidence: 97%

Effect of BMP4 preceded by retinoic acid and co‐culturing ovarian somatic cells on differentiation of mouse embryonic stem cells into oocyte‐like cells

et al. 2015

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“…To mimic a suitable microenvironment for the development of germ cells, co-culture systems or conditioned medium have been used for differentiating hESCs/ iPSCs. Co-culture with human fetal gonadal stromal cells (64), mouse Sertoli cells (65), mouse embryonic fibroblasts (66), or porcine ovarian fibroblasts (45) increased the efficiency of germ cell-like differentiation from hESCs/iPSCs. These results confirm that specific trophic factors are required to develop germline from pluripotent stem cells.…”

Section: Toward Efficient Differentiation Into Germline By Specific Cmentioning

confidence: 99%

Germline development from human pluripotent stem cells toward disease modeling of infertility

Hayashi

Saitou

Yamanaka

2012

Fertility and Sterility

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“…The first report of germ-like cells from hiPSCs suggests that it may be easier to derive germ cell-like cells from human iPSCs than from hESCs (Park et al, 2009). The use of co-culture systems or conditioned medium, increases the efficiency of human PGC-like cells derivation suggesting that yet unknown factors are required to develop germline cells from pluripotent stem cells (Geens et al, 2011;Park et al, 2009;Richards et al, 2010;West et al, 2008). In an attempt to define the most suitable conditions for the differentiation of germ cells from hESCs/iPSCs, various cytokines and signaling molecules have been tested.…”

Section: Critical Advances For the Study Of Pgc Specificationmentioning

confidence: 99%

Perceiving signals, building networks, reprogramming germ cell fate

Barrios¹,

Irie²,

Surani³

2013

Int. J. Dev. Biol.

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Germ cell development is a step-wise process that ensures the progression of the life cycle due to their unique ability to transmit their genome from one generation to the next. In the mouse, the precursors of germ cells, the Primordial Germ Cells (PGCs), arise at the onset of gastrulation. Here we discuss how PGCs acquire their fate in the epiblast and outline their development until their arrival into the gonads. Male germ cell tumors (GCTs) have a similar gene expression pattern to that of fetal germ cells and to pluripotent cells, suggesting that GCT originate from an alteration of gonocyte normal development. We evaluate coincidences and differences in germ cell development in mouse and humans and on this basis, we speculate future research perspectives.

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Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells

Cited by 27 publications

References 34 publications

Effect of BMP4 preceded by retinoic acid and co‐culturing ovarian somatic cells on differentiation of mouse embryonic stem cells into oocyte‐like cells

Effect of BMP4 preceded by retinoic acid and co‐culturing ovarian somatic cells on differentiation of mouse embryonic stem cells into oocyte‐like cells

Germline development from human pluripotent stem cells toward disease modeling of infertility

Perceiving signals, building networks, reprogramming germ cell fate

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