Sertoli cell–conditioned medium restores spermatogenesis in azoospermic mouse testis

Panahi, Sakineh; Karamian, Amin; Sajadi, Ensieh; Aliaghaei, Abbas; Nazarian, Hamid; Abdi, Shabnam; Danyali, Samira; Paktinat, Shahrokh; Abdollahifar, Mohammad‐Amin; Farahani, Reza Mastery

doi:10.1007/s00441-019-03092-w

Cited by 26 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study for the rst time indicated the effects of BMCM for the healing of chemotherapy disrupted the germinal layer in mice. Consistent with our ndings, applications of Sertoli cell-CM on the azoospermia model induced spermatogenesis in mice (33). In addition, 10% of the CM from canine amniotic membrane-derived MSCs enhanced the quality of frozen-thawed sperm cells (34).…”

Section: Discussionsupporting

confidence: 89%

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Zhankina

Afshar

Farrar

et al. 2022

Preprint

View full text Add to dashboard Cite

Background One of the main cause of male infertility is non-obstructive azoospermia, which is not manageable medically. The first aim of the current research was to show the effect of extracellular vesicle-contained conditioned media (CM) instead of mesenchymal stromal/stem cells (MSCs) for treatment of non-obstructive azoospermia. In the next step, we aimed to study the differentiation potential of MSCs into spermatocytes after injection of MSCs in mice seminiferous tubules. This study has provided an applied treatment for busulfan-induced azoospermia using adipose tissue-derived (AT-MSCs) and bone marrow-derived MSCs (BM-MSCs) and bone marrow CM (BMCM) in animal models. Methods Thirty male adult Balb/C mice (30±5 g) and two female eGFP+/+Balb/C mice (30±5 g) were used to design experimental groups and to culture stem cells, respectively. Then, six groups including intact control, azoospermia, AT-MSC therapy, BM-MSC therapy, BMCM therapy, and spontaneous healing groups were considered. All groups except intact control were induced azoospermia by injecting two doses of busulfan (10 mg/kg) with 21 days’ interval. Testes of all mice were removed and studied through histomorphometry and flow cytometry analysis 60 days after treatment. Results Histomorphometry and flow cytometry evaluation of testes showed normal morphology of most of the seminiferous tubules of therapy groups as well as successful recovery of spermatogenesis, but spermatogenesis was not observed in the azoospermia group. It is worth notable that the results of the BM-MSC therapy group were more favorable than other therapy groups. Conclusions AT-MSC, BM-MSC and BMCM can be strongly suggested as candidates in the therapy of azoospermia.

show abstract

Section: Discussionsupporting

confidence: 89%

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Zhankina

Afshar

Farrar

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Our study for the rst time indicated the effects of BMCM for the healing of chemotherapy disrupted the germinal layer in mice. Consistent with our ndings, applications of Sertoli cell-CM on the azoospermia model induced spermatogenesis in mice (Panahi et al, 2020). In addition, 10% of the CM from canine amniotic membrane-derived MSCs enhanced the quality of frozen-thawed sperm cells (Mahiddine et al, 2020).…”

Section: Discussionsupporting

confidence: 89%

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Zhankina

Afshar

Farrar

et al. 2021

Preprint

View full text Add to dashboard Cite

One of the main cause of male infertility is non-obstructive azoospermia, which is not manageable medically. The first aim of the current research was to show the effect of extracellular vesicle-contained conditioned media (CM) instead of mesenchymal stromal/stem cells (MSCs) for treatment of non-obstructive azoospermia. In the next step, we aimed to study the differentiation potential of MSCs into spermatocytes after injection of MSCs in mice seminiferous tubules. This study has provided an applied treatment for busulfan-induced azoospermia using adipose tissue-derived (AT-MSCs) and bone marrow-derived MSCs (BM-MSCs) and bone marrow CM (BMCM) in animal models. In this regard, 30 male adult Balb/C mice (30±5g) and two female eGFP+/+ Balb/C mice (30±5g) were used to design experimental groups and to culture stem cells, respectively. Then, six groups including intact control, azoospermia, AT-MSC therapy, BM-MSC therapy, BMCM therapy, and spontaneous healing groups were considered. All groups except intact control were induced azoospermia by injecting two doses of busulfan (10 mg/kg) with 21 days’ interval. Testes of all mice were removed and studied through histomorphometry and flow cytometry analysis 60 days after treatment. Histomorphometry and flow cytometry evaluation of testes showed normal morphology of most of the seminiferous tubules of therapy groups as well as successful recovery of spermatogenesis, but spermatogenesis was not observed in the azoospermia group. It is worth notable that the results of the BM-MSC therapy group were more favorable than other therapy groups. Consequently, AT-MSC, BM-MSC and BMCM can be strongly suggested as candidates in the therapy of azoospermia.

show abstract

“…The number of the selected seminiferous tubule profiles was counted using an unbiased counting frame. The length density of seminiferous tubules was also estimated using the following formula [ 13 , 14 ]: where ΣQ is the total number of the seminiferous tubules, a/f is the area per frame and ΣP is the total points superimposed on the testis tissue. The total length of the seminiferous tubules was estimated by multiplying the length density (L v ) by the total volume of the testis.…”

Section: Methodsmentioning

confidence: 99%

“…Using a projecting microscope, the live image of 8–10 testis sections was evaluated. The volumes were calculated using the Cavalieri method [ 13 , 14 ]. Using stereological software, a point grid was superimposed on the testis images.…”

Section: Methodsmentioning

confidence: 99%

COVID-19 disrupts spermatogenesis through the oxidative stress pathway following induction of apoptosis

et al. 2021

Self Cite

View full text Add to dashboard Cite

To evaluate the incidence of apoptosis within the testes of patients who died from severe acute respiratory syndrome coronavirus 2 (COVID-19) complications, testis tissue was collected from autopsies of COVID-19 positive (n = 6) and negative men (n = 6). They were then taken for histopathological experiments, and RNA extraction, to examine the expression of angiotensin-converting enzyme 2 (ACE2), transmembrane protease, serine 2 (TMPRSS2), BAX, BCL2 and Caspase3 genes. Reactive oxygen species (ROS) production and glutathione disulfide (GSH) activity were also thoroughly examined. Autopsied testicular specimens of COVID-19 showed that COVID-19 infection significantly decreased the seminiferous tubule length, interstitial tissue and seminiferous tubule volume, as well as the number of testicular cells. An analysis of the results showed that the Johnsen expressed a reduction in the COVID-19 group when compared to the control group. Our data showed that the expression of ACE2, BAX and Caspase3 were remarkably increased as well as a decrease in the expression of BCL2 in COVID-19 cases. Although, no significant difference was found for TMPRSS2. Furthermore, the results signified an increase in the formation of ROS and suppression of the GSH activity as oxidative stress biomarkers. The results of immunohistochemistry and TUNEL assay showed that the expression of ACE2 and the number of apoptotic cells significantly increased in the COVID-19 group. Overall, this study suggests that COVID-19 infection causes spermatogenesis disruption, probably through the oxidative stress pathway and subsequently induces apoptosis.

show abstract

Sertoli cell–conditioned medium restores spermatogenesis in azoospermic mouse testis

Cited by 26 publications

References 20 publications

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

COVID-19 disrupts spermatogenesis through the oxidative stress pathway following induction of apoptosis

Contact Info

Product

Resources

About