Sertoli cell signaling by Desert hedgehog regulates the male germline

Bitgood, Mark Joseph; Shen, Liya; McMahon, Andrew P.

doi:10.1016/s0960-9822(02)00480-3

Cited by 600 publications

(472 citation statements)

References 32 publications

Supporting

Mentioning

447

Contrasting

Unclassified

Order By: Relevance

“…Paramantier et al12 found that the peripheral nerves of dhh null mutant mice showed minifascicular formation. Analysis of null mutant mice demonstrated that dhh signaling plays an essential role in the regulation of mammalian spermatogenesis 13. Because mammalian hedgehog genes share a striking homology to the Drosophila segment polarity hedgehog gene, these findings suggest that DHH plays crucial roles in both peripheral nerve sheath development and spermatogenetic regulation in humans.…”

Section: Discussionmentioning

confidence: 98%

Partial duplication of DHH causes minifascicular neuropathy

Sato

Maekawa

Ishiura

et al. 2017

Ann Clin Transl Neurol

View full text Add to dashboard Cite

Minifascicular neuropathy (MN) is an extremely rare developmental malformation in which peripheral nerves are composed of many small fascicles. Only one patient with MN with 46XY gonadal dysgenesis (GD) was found to carry a mutation affecting the start codon in desert hedgehog (DHH). We identified an identical novel rearrangement mutation of DHH in two consanguineous families with MN, confirming mutations in DHH cause MN with 46XY GD. The patients with the 46XY karyotype developed GD, whereas a patient with the 46XX karyotype did not. These findings further support that DHH has important roles in perineural formation and male gonadal differentiation.

show abstract

Section: Discussionmentioning

confidence: 98%

Partial duplication of DHH causes minifascicular neuropathy

Sato

Maekawa

Ishiura

et al. 2017

Ann Clin Transl Neurol

View full text Add to dashboard Cite

show abstract

“…Unlike SHH, which is required for the development of seemingly all organs, the role of IHH and DHH is restricted to a more limited number of tissue-specific developmental events, e.g. bone morphology and gonadal differentiation, respectively (Bitgood et al, 1996;St-Jacques et al, 1999).…”

Section: Hh Signaling: a Master Regulator Of Developmentmentioning

confidence: 99%

Roles for Hedgehog signaling in adult organ homeostasis and repair

2014

View full text Add to dashboard Cite

The hedgehog (HH) pathway is well known for its mitogenic and morphogenic functions during development, and HH signaling continues in discrete populations of cells within many adult mammalian tissues. Growing evidence indicates that HH regulates diverse quiescent stem cell populations, but the exact roles that HH signaling plays in adult organ homeostasis and regeneration remain poorly understood. Here, we review recently identified functions of HH in modulating the behavior of tissue-specific adult stem and progenitor cells during homeostasis, regeneration and disease. We conclude that HH signaling is a key factor in the regulation of adult tissue homeostasis and repair, acting via multiple different routes to regulate distinct cellular outcomes, including maintenance of plasticity, in a context-dependent manner.

show abstract

“…Within this cell population we identified genes known to be important for testes development or as preSertoli cell markers, including Sox9, Amh/Mis, Dhh, Sf1/Nr5a1, Gata4, Gata6, Dmrt1, Fgf9, and Ptgds (Ikeda et al, 1994;Bitgood et al, 1996;Kent et al, 1996;Morais da Silva et al, 1996;Ketola et al, 1999;Colvin et al, 2001;Adams and McLaren, 2002;Tevosian et al, 2002;Malki et al, 2005;Wilhelm et al, 2005). Notably, HybSRYp-RFP expressing cells did not express markers for germ cells, such as Oct4 and Ssea-1, or markers for endothelial cells, such as Ntrk3.…”

Section: Fluorescent Hybsryp-rfp Cells Express Presertoli Cells Markementioning

confidence: 99%

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Cory

Boyer

Pilon

et al. 2007

Molecular Reproduction Devel

View full text Add to dashboard Cite

In mammals, the pre-Sertoli cell of the male genital ridge is the first cell type to display sex specific differentiation and differential gene expression. The genetic cascade driving the differentiationof pre-Sertoli cells and ultimately testis formationis beginning to be unravelled, but many questions remain. A better understanding of the transcriptome of pre-Sertoli cells immediately after sex determination is essential in order to further understand this differentiationprocess. A mouse model expressing Red Fluorescent Protein (RFP) under the control of a hybrid mouse/pig SRY promoter (HybSRYp-RFP) was used to purify cells from embryonic day 12.0 (e12.0) male genital ridges. To compare the transcriptomes of HybSRYp-RFP cell populations versus age matched whole female genital ridges, RNA was extracted and used to generate molecular probes that were hybridized onto Affymetrix Mouse Genome 430 2.0 microarrays. The expression of genes considered markers for pre-Sertoli cells, including Sox9, Mis, Dhh and Fgf9 were identified within the HybSRYp-RFP expressing cell population, while markers for germ cells (Oct4, SSEA-1) and endothelial cells (Ntrk3) were not identified. In contrast, markers for ovarian somatic cell expression, including Fst and Bmp2, were identified as overexpressed within the ovarian cell population. In a general fashion, genes identified as 2.5-fold over expressed in HybSRYp-RFP expressing cells coded notably for cell signalling and extra cellular proteins. The expression of Sox10, Stc2, Fgf18, Fgf13 and Wnt6 were further characterized via whole mount in situ hybridization (WISH) on male and female genital ridges between e11.5 and e14.5. Sox10, Fgf18, Fgf13 and Stc2 gene expression was detected within the male genital ridges while Wnt6 was found diffusely within both the male and female genital ridges. These data represent the earliest comprehensive microarray expression analysis of purified presumptive pre-Sertoli cells available to date. Mol. Reprod. Dev. 74: 1491-1504, 2007

show abstract

Sertoli cell signaling by Desert hedgehog regulates the male germline

Cited by 600 publications

References 32 publications

Partial duplication of DHH causes minifascicular neuropathy

Partial duplication of DHH causes minifascicular neuropathy

Roles for Hedgehog signaling in adult organ homeostasis and repair

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Contact Info

Product

Resources

About