Sertoli Cell-specific Expression of Metastasis-associated Protein 2 (MTA2) Is Required for Transcriptional Regulation of the Follicle-stimulating Hormone Receptor (FSHR) Gene during Spermatogenesis

Zhang, Shun; Li, Wei; Zhu, Chuchao; Wang, Xiaohong; Li, Zhen; Zhang, Jinshan; Zhao, Jie; Hu, Jing; Teng, Li; Zhang, Yuanqiang

doi:10.1074/jbc.m112.383802

Cited by 68 publications

(37 citation statements)

References 40 publications

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“…31, 32, 33 A number of putative binding sites for transcription factors were detected in the 5' regulatory region of mammalian FSHR genes. Some of these transcription factors, such as GATA-1, 34 E2F, 34 SF-1, 35 and USF-1, 36 regulate FSHR expression by binding to its 5' regulatory region.…”

Section: Discussionmentioning

confidence: 99%

TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis

et al. 2016

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Follicle-stimulating hormone receptor (FSHR) and its intracellular signaling control mammalian follicular development and female infertility. Our previous study showed that FSHR is downregulated during follicular atresia of porcine ovaries. However, its role and regulation in follicular atresia remain unclear. Here, we showed that FSHR knockdown induced porcine granulosa cell (pGC) apoptosis and follicular atresia, and attenuated the levels of intracellular signaling molecules such as PKA, AKT and p-AKT. FSHR was identified as a target of miR-143, a microRNA that was upregulated during porcine follicular atresia. miR-143 enhanced pGC apoptosis by targeting FSHR, and reduced the levels of intracellular signaling molecules. SMAD4, the final molecule in transforming growth factor (TGF)-β signaling, bound to the promoter and induced significant downregulation of miR-143 in vitro and in vivo. Activated TGF-β signaling rescued miR-143-reduced FSHR and intracellular signaling molecules, and miR-143-induced pGC apoptosis. Overall, our findings offer evidence to explain how TGF-β signaling influences and FSHR signaling for regulation of pGC apoptosis and follicular atresia by a specific microRNA, miR-143.

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Section: Discussionmentioning

confidence: 99%

TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis

et al. 2016

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“…Western blotting was carried out as described in our previous work. 49 Protein samples were prepared in ice-cold RIPA buffer (Tris-HCl 50 mM, NaCl 150 mM, Triton X-100 1% (vol/vol), sodium deoxycholate 1% (wt/vol) and SDS 0.1% (wt/vol), pH 7.5) supplemented with complete proteinase-inhibitor cocktail tablets (Roche Diagnostic, Shanghai, China). Approximately, 20 μg of total protein was separated on SDS-PAGE and transferred to nitrocellulose membrane (Millipore, Bedford, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…49 Briefly, after deparaffinization and rehydration, antigen retrieval was performed by incubating slides in 0.01% citrate acid in a preheated water bath (95-100°C) for 30 min and then cooling to room temperature. Sections were then exposed to 0.3% hydrogen peroxide in methanol for 10 min to destroy endogenous peroxides activity, followed by incubation with primary antibodies at 4°C overnight in a moist box.…”

Section: Methodsmentioning

confidence: 99%

Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells

Hou

et al. 2015

Cell Death Differ

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“…Another member of the MTA family, MTA2, is induced by testosterone as well as by follicle-stimulating hormone (FSH) in Sertoli cells. Once induced, MTA2 also represses the transcription of the FSH receptor in FSH stimulated cells as a part of a negative feedback regulatory loop [38]. Consist with this pathway, experimental downregulation of MTA2 impairs the FSH-mediated downregulation of the FSH receptor [38].…”

Section: Mta Proteins In Mammalsmentioning

confidence: 99%

Physiological functions of MTA family of proteins

Sen

Gui

Kumar

2014

Cancer Metastasis Rev

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Although the functional significance of the MTA family of chromatin remodeling proteins in the pathobiology of cancer is fairly well recognized, the physiological role of MTA proteins continues to be an understudied research area and is just beginning to be recognized. Similar to cancer cells, MTA1 also modulates the expression of target genes in normal cells either by acting as a corepressor or coactivator. In addition, physiological functions of MTA proteins are likely to be influenced by its differential expression, subcellular localization and regulation by upstream modulators and extracellular signals. This review summarizes our current understanding of physiological functions of the MTA proteins in model systems. In particular, we highlight recent advances of the role MTA proteins play in the brain, eye, circadian rhythm, mammary gland biology, spermatogenesis, liver, immunomodulation and inflammation, cellular radio-sensitivity, and hematopoiesis and differentiation. Based on the growth of knowledge regarding the exciting new facets of the MTA family of proteins in biology and medicine, we speculate that the next burst of findings in this field may reveal further molecular regulatory insights of non-redundant functions of MTA coregulators in the normal physiology as well as in pathological conditions outside cancer.

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Sertoli Cell-specific Expression of Metastasis-associated Protein 2 (MTA2) Is Required for Transcriptional Regulation of the Follicle-stimulating Hormone Receptor (FSHR) Gene during Spermatogenesis

Cited by 68 publications

References 40 publications

TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis

TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis

Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells

Physiological functions of MTA family of proteins

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