Serum adiponectin and bone mineral density in male hemodialysis patients

Abstract

Increased levels of serum adiponectin were associated with decrease in BMD in male hemodialysis patients. Adiponectin may play a role in mineral and bone disorder, possibly in bone resorption, of patients with CKD 5D.

Significant Association Between Bone-Specific Alkaline Phosphatase and Vascular Calcification of the Hand Arteries in Male Hemodialysis Patients

“…As serum bone resorption markers, serum cross-linked N-telopeptide of type I collagen (NTX) and C-terminal telopeptide of type I collagen (CTX) were measured. Serum NTX was measured by ELISA (Osteomark NTX serum; Ostex International, Seattle, WA, USA), with an intra-assay CV of 4.6%, as reported previously [22,23]. Serum CTX was determined by means of the Elecsys β-CrossLaps/serum assay (Roche Diagnostics, Mannheim, Germany), which is a sandwich immunoassay with two monoclonal antibodies specific for the β-isomerized 8-amino acid sequence of the C-terminal telopeptide of type I collagen [24].…”

“…The serum samples were stored in aliquots at −20°C until subsequent assay of bone metabolism markers, with measurements made immediately after thawing. The serum biochemical parameters for bone metabolism were determined, as described previously [22,23,24,25]. All serum bone markers were measured in the same assay run, in order to avoid inter-assay variance.…”

“…As markers of bone formation, serum BAP, N-Terminal propeptide of type I collagen (P1NP), and intact osteocalcin (OC) were measured. Serum BAP was measured, using an enzyme monoclonal antibody used immunoassay kit (Alkphase-B; Metra Biosystem, Mountain View, CA, USA), as reported previously [22,26,27]. Intra-assay and inter-assay CVs were 2.2% and 3.1%, respectively [22].…”

“…Serum BAP was measured, using an enzyme monoclonal antibody used immunoassay kit (Alkphase-B; Metra Biosystem, Mountain View, CA, USA), as reported previously [22,26,27]. Intra-assay and inter-assay CVs were 2.2% and 3.1%, respectively [22]. Serum P1NP was measured using a competitive radioimmunoassay kit (Orion Diagnostica, Oulunsalo, Finland) [25].…”

“…The mean values of six measurements of calcium and phosphate during the 3 months prior to roentgenography were used for analysis. Serum intact parathyroid hormone (intact PTH) was measured by electrochemiluminescence immunoassay (Elecsys PTH, Roche Diagnostics GmbH, Mannheim, Germany) [22]. Serum intact PTH was measured once, at the time of roentgenography.…”