Serum Albumin

Peters, Theodore

doi:10.1016/s0065-2423(08)60385-6

Cited by 488 publications

(571 citation statements)

References 397 publications

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“…On the basis of morphological obser vations (discussed later in the text) it is suggested that the value of (J is probably low. Edsal, 1953;Putnam, 1965;and Peters, 1970. c Source of data Groothuis et ai ., 1983.…”

Section: Discussionmentioning

confidence: 99%

“…where PS is the permeability-surface area product of the capillaries, 8 is the average thickness of the capillary wall (= 100 A) (Coomber and Stewart, 1985;Molnar et al, unpublished observations), D is the aqueous diffusion constant of albumin (6.4 cm2/s x 10-7; value corrected to 37°C) (Edsal, 1953;Putnam, 1965;Peters, 1970), a is the average radius of the albumin mole cule (3 nm),1 p is the radius of the pore, and N is the number of pores per unit mass of tissue. If K/D is ap proximately constant (i.e., varies by <30%) for different size molecules, then the "holes" or "pores" in the capil lary membrane must be large enough so that the steric hinderance factor is negligible for these molecules.…”

Section: (5)mentioning

confidence: 99%

“…Plasma-derived fluid flow in the ECS is also assumed to extend across the tumor margin, into and through adjacent brain paren chyma; but no significant fluid transfer is assumed to occur across normal brain capillaries during the experi mental period. It has also been assumed that the extra cellular space of tumor and adjacent brain are the same 1 The tertiary structure and hydrodynamic properties of al bumin have been extensively studied; it is generally agreed that the overall dimensions of the molecule approximates a prolate ellipsoid with a 3.5 to I axial ratio (14 x 4 nm) (Edsal, 1953;Putnam, 1965;Peters, 1970) . The molecule has a radius of gyra tion in solution of 2.98 nm.…”

Section: (5)mentioning

confidence: 99%

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Dexamethasone Effects on [¹²⁵I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Nakagawa¹,

Groothuis

Owens³

et al. 1987

J Cereb Blood Flow Metab

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Summ ary: A total of 72 RG-2 transplanted gliomas were studied in 58 rats at three time points (1, 30, 240 min) after intravenous injection of [125Ilradioiodinated serum albumin ([125I]RISA). The animals were divided into two groups: a control group that received no treatment and a second group that was treated with five doses of 1.5 mg/kg of dexamethasone over 2.5 days. 687comparison of the tumor influx constants in control an imals and the aqueous diffusion constants of two different size molecules (RISA and amino isobutyric acid) suggests that the "pores" across RG-2 capillaries are large and may not restrict the free diffusion of RISA (estimated minimum pore diameter > 36 nm) and that the total pore area is -6.2 x 10-5 cm2 g-I in RG-2 tumor tissue. The second model, which allows for diffusion and solvent drag of RISA across tumor capillaries and through the tissue, was used to analyze the distribution profiles of RISA in peripheral tumor and adjacent brain. This anal ysis was consistent with a small bulk flow of plasma-de rived edema fluid (capillary filtration rate = 0.8 I·d g-I min -I) and a larger component of free diffusion of RISA ( K = 2 fLl g-I min-I) through pores in the tumor vessels of control animals. Dexamethasone treatment markedly reduced or eliminated the filtration of plasma-derived fluid across tumor capillaries and the movement of RISA through the extracellular space by solvent drag. These effects may be mediated by a reduction in the size of the extracellular space and/or a decrease in the pore size of tumor capillaries and could represent an important mech anism for corticosteroid control of tumor and peri tumoral brain edema.

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Section: Discussionmentioning

confidence: 99%

Section: (5)mentioning

confidence: 99%

Section: (5)mentioning

confidence: 99%

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Dexamethasone Effects on [¹²⁵I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Nakagawa¹,

Groothuis

Owens³

et al. 1987

J Cereb Blood Flow Metab

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“…Each assay contained 20 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (Hepes) (pH 7.6), 2 mM dithiothreitol, 85 mM KCI, 1.75 mM Mg(OAc)2, 75 MAM spermine, creatine kinase at 80,ag/ml, 1 mM ATP, 0.4 mM GTP, 15 (27).…”

Section: Methodsmentioning

confidence: 99%

Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.

Strauss¹,

Donohue²,

Bennett³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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Rat liver poly(A-containing RNA greatly stimulated incorporation of radioactive amino acids into protein when added to a wheat germ in vitro translation system. Approximately 7% of the labeled synthetic product was precipitated following indirect immunoprecipitation with antisera to rat serum albumin. Analysis of this material, and of the cyanogen bromide fragments derived from it, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed that it contained an NH2-terminal extension of about 2500 daltons when compared to rat serum albumin. Automated sequence determination of purified cell-free product labeled with various radioactive amino acids revealed the presence of 18 additional amino acids NH2-terminal to the sequence of rat proalbumin. The partial sequence of this extension was found to be: Met-

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“…In this study, we demonstrate the integration of BSA in the glass microfluidic channels. BSA is an abundant, stable, and soluble protein from blood plasma [17], which is routinely used in 3D microfabrication [8][9][10][11][12][13][14][15]. As a photoinitiator, we utilize (Sodium 4-[2-(4-Morpholino) benzoyl-2-dimethylamino] butylbenzenesulfonate) (MBS) because it is a water-soluble variant of the widely used Irgacure 369 with a broad UV absorption peak [18,19].…”

Section: Introductionmentioning

confidence: 99%

Femtosecond Laser Direct Write Integration of Multi-Protein Patterns and 3D Microstructures into 3D Glass Microfluidic Devices

et al. 2018

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Microfluidic devices and biochips offer miniaturized laboratories for the separation, reaction, and analysis of biochemical materials with high sensitivity and low reagent consumption. The integration of functional or biomimetic elements further functionalizes microfluidic devices for more complex biological studies. The recently proposed ship-in-a-bottle integration based on laser direct writing allows the construction of microcomponents made of photosensitive polymer inside closed microfluidic structures. Here, we expand this technology to integrate proteinaceous two-dimensional (2D) and three-dimensional (3D) microstructures with the aid of photo-induced cross-linking into glass microchannels. The concept is demonstrated with bovine serum albumin and enhanced green fluorescent protein, each mixed with photoinitiator (Sodium 4-[2-(4-Morpholino) benzoyl-2-dimethylamino] butylbenzenesulfonate). Unlike the polymer integration, fabrication over the entire channel cross-section is challenging. Two proteins are integrated into the same channel to demonstrate multi-protein patterning. Using 50% w/w glycerol solvent instead of 100% water achieves almost the same fabrication resolution for in-channel fabrication as on-surface fabrication due to the improved refractive index matching, enabling the fabrication of 3D microstructures. A glycerol-water solvent also reduces the risk of drying samples. We believe this technology can integrate diverse proteins to contribute to the versatility of microfluidics.

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Serum Albumin

Cited by 488 publications

References 397 publications

Dexamethasone Effects on [¹²⁵I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Dexamethasone Effects on [¹²⁵I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.

Femtosecond Laser Direct Write Integration of Multi-Protein Patterns and 3D Microstructures into 3D Glass Microfluidic Devices

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Serum Albumin

Cited by 488 publications

References 397 publications

Dexamethasone Effects on [125I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Dexamethasone Effects on [125I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.

Femtosecond Laser Direct Write Integration of Multi-Protein Patterns and 3D Microstructures into 3D Glass Microfluidic Devices

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Product

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Dexamethasone Effects on [¹²⁵I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain

Dexamethasone Effects on [¹²⁵I]Albumin Distribution in Experimental RG-2 Gliomas and Adjacent Brain