Serum alkaline phosphatase activity during zinc deficiency and long-term inflammatory stress

Abstract

A decrease in serum zinc can be caused by a real zinc deficiency but can also be caused by an apparent zinc deficiency, e.g. in inflammatory stress. The aim of this study was to evaluate the diagnostic power of serum alkaline phosphatase (AP) activity in the discrimi nation between pathophysiologic states of "real" and "apparent" zinc deficiency. A decrease in serum zinc was induced in growing and adult rats, by providing a diet low in zinc and by causing inflammatory stress. AP activity was determined using reagents low or enriched in zinc. Serum AP was decreased in zinc-deficient adult rats (P < 0.01). In zinc-deficient growing rats AP activity was not different from normal rats but AP activity decreased rapidly. In the same growing rats a significant difference was found in AP activities determined using buffers low and enriched in zinc (P < 0.001) between both groups of rats. After inducing inflammatory stress a decrease in AP activity (P < 0.01) and serum zinc (P < 0.001) was seen during the first few days. After the initial phase of inflammation AP activity normalized, serum zinc showed a rise which after correction for the decrease in serum albumin reached the level of the control rats. A difference in AP activity in buffers low and enriched in zinc was observed only during the first few days after induction of inflammatory stress (P < 0.001). Probably the method of measurement of the difference in (1996) [109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][124][125][126][127] enzyme activity, using buffers low and enriched in zinc, can be used as an indication for zinc deficiency in situations with changing AP enzyme concentrations. AP activity is decreased during the initial phase of inflammatory stress due to a decrease in serum zinc.

Effect of Zinc Supplementation in Children with Acute Diarrhea: Randomized Double Blind Controlled Trial

“…Serum alkaline phosphatase measurement has been suggested as an important surrogate marker of zinc status in human [14]. In present study, it has been shown that serum alkaline phosphatase was low in all patients, may be correlating with zinc deficiency, but we were not able to do the follow up study on the seventh and fourteenth day supplementation.…”

Zinc Supplementation in Malnourished Children With Persistent Diarrhea in Pakistan

“…Serum alkaline phosphatase measurement has been suggested as an important surrogate marker of zinc status in humans. 37,38 The serial rise of serum alkaline phosphatase values in supplemented children suggests that sufficient absorption and replenishment of body zinc occurred with the dose of zinc used.…”

The cognitive impairment induced by zinc deficiency in rats aged 0∼2 months related to BDNF DNA methylation changes in the hippocampus

“…[22][23][24] Because zinc deprivation Data are expressed as mean ± SD, n = 10. [25][26][27] Hence, the ALP activity is a good indicator to evaluate the status of zinc deficiency in growing rats. † Difference from the PF group, P < 0.05.…”