Serum and Synovial Fluid Proteins in Arthritis

Wilkinson, Matthew; Jones, Brian S.

doi:10.1136/ard.21.1.51

Cited by 30 publications

(13 citation statements)

References 10 publications

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“…Synthesis or destruction in the joint can then be inferred by deviations from the expected value. Similar reasoning has been employed by others (34,35) in studies of rheumatoid factors and y-globulin in synovial fluid. T h e usefulness of this approach was tested by determining SF:S ratios of IgG and IgM for each group of patients and comparing them with what would be expected on the basis of the molecular size of these molecules.…”

Section: Discussionmentioning

confidence: 71%

Permeability of human synovial membrane to plasma proteins. Relationship to molecular size and inflammation

Kushner

Somerville

1971

Arthritis & Rheumatism

181

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Passage of plasma proteins from blood into synovial fluid was found quantitatively related to molecular size and to degree of inflammation in four groups of patients differing in severity of synovial inflammation, and in a group of autopsied subjects without joint disease. Greater inflammation was accompanied by augmented passage of each protein across the synovial membrane, and by proportionately greater increase in permeability to larger proteins. In classic rheumatoid arthritis, synovial IgM and IgG concentrations were higher than predicted from the above-described interrelationships, suggesting local immunoglobulin synthesis.The proteins of synovial fluid are derived from plasma proteins, with the exception of a few constituents present in very low concentration (1-3). It is known that passage of a protein into the synovial space through synovial vascular walls and interstitial hyaluronate is affected by the molecular size of the protein and by the presence

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Section: Discussionmentioning

confidence: 71%

Permeability of human synovial membrane to plasma proteins. Relationship to molecular size and inflammation

Kushner

Somerville

1971

Arthritis & Rheumatism

181

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“…Different activity zones represented a considerable quantity of protein fractions, however albumin remained the main fraction in the sera of control as well as the subjects with OA. Published reports have shown the occurrence of twenty fractions in the synovial fluid of patients with osteoarthritis and demonstrated albumin as the major fraction (16,33). The reason for the presence of an extra peptide in the b-globulin region may be either attributed to the sensitivity of the software used or the number of OA subjects analysed during the present investigation.…”

Section: Discussionmentioning

confidence: 62%

“…Available literature suggests that the synovial fluid protein profiles are most often fluctuating and could not be reproduced many times (33). However, the proteins present in synovial fluid and serum have been shown to be qualitatively identical (34).…”

Section: Discussionmentioning

confidence: 99%

“…Various studies have identified potential biochemical markers as a means of diagnosing and monitoring OA, and investigators in some of these studies have reported radiographic assessment (35), hyaluronan (36), C-reactive proteins (37)(38), enzymes (20, 22-23, 25, 39-41) and many more (42)(43)(44)(45)(46). Even the estimation of absolute protein values provides relatively better options and puts forward the marker value of many useful protein fractions (16,33,35,47).…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Serum Proteins and Enzymes Level in Human Subjects with Osteoarthritis

Ahmad¹,

Irshad²,

Ahmad³

et al. 2011

Journal of Medical Biochemistry

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Kratak sadr`aj: Cilj ove studije bio je da se pomo}u profila PAGE odrede nivoi proteina i enzima u serumu subjekata sa osteoartritisom (OA). Za studiju je izabrana 41 osoba sa potvr|enom dijagnozom OA. Uzorci seruma su u jednakim koli~inama zasebno stavljeni na PAGE sa i bez denaturacije. Softverska analiza ovih profila obavljena je pomo}u pro grama Scion Imaging (Beta release-4, Scion Corporation) and GelPro (Media Cybernetics, SAD). Radi vizualizacije esteraza i (Est) izoenzima laktat dehidrogenaze (LDH) u serumu ovih pacijenata izvr{eno je bojenje specifi~nim supstratom. Razlike u vrednostima kontrolnih i osoba sa OA su statisti~ki testirane. Softverska analiza PAGE profila bez denaturacije otkrila je prisustvo devetnaest peptida u kontroli i dvadeset jednog u osobama sa OA. Dva dodatna peptida bila su prisutna u regionu gama-globulina kod osoba sa OA. Zna~ajan pad sa 42,77% na 36,72 % nivoa albumina (hipoalbuminemia) prime}en je kod osoba sa OA a odnos ukupnog albumina i globulina bio je 0,58. U SAD-PAGE, razlika izme|u kontrola i osoba sa OA uo~ena je izme|u osam peptida molekularne te`ine 25, 22 i 20 kDa (kojih nema u OA) i pet novih peptida od 270, 125, 30, 21,36 i 18,5 kDa (kojih nema kod kontrola), dok je albumin zadr`ao najve}u aktivnost. Za enzime, Est prate relativni poredak BchEst (42,86%) > ArylEst (16,24%) > AchEst (6,85%) kod osoba sa OA uz ekspresiju nove izoforme BchEst sa 4,78% i dve izoforme ArylEst u koncentracijama od 2,13 i 1,61%. Esterazi sli~na aktivnost albumina (AlbEst) bila je zna~ajno (P<0,05) sni`ena (34%) kod obolelih u pore|enju sa kontrolom (47%). Zna~ajan porast LDH-5 i pad LDH-1 i -2 izoenzima tako|e su prime}eni Summary: The aim of the present study was to assess the serum proteins and enzymes level using polyacrylamide gel electrophoretic (PAGE) profiles in human subjects with osteo arthritis (OA). Forty-one subjects with confir med OA were selected for the present study. Sera were collected from these individuals and loaded in equal amounts on native and denaturing PAGE separately. Software analysis of these profiles was done using Scion Imaging (Beta release-4, Scion Corporation) and GelPro (Media Cybernetics, USA) programs. To visualize esterases (Est) and lactate dehydro genase (LDH) isoenzymes in the sera of these patients substrate specific staining was performed. Differences in the values of control and OA subjects were tested statistically. Software analysis of native-PAGE profiles revealed the presence of nineteen peptides in control and twenty one in OA subjects respectively. Two extra peptides were present in the b-globulins region of OA subjects. Significant decline from 42.77% to 34.72% in albumin levels (hypoalbuminemia) was observed in OA subjects with total albumin to globulin ratio 0.58. In SDS-PAGE, the difference in control and OA subjects was observed among eight peptides with molecular weight 25, 22 and 20 kDa (absent in OA) and five novel peptides 270, 125, 30, 21.36 and 18.4 kDa (absent in controls), while albumin retains the major activity. For enzymes...

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“…Synovial fluid is generally considered to be a dialysate of plasma to which mucin has been added The proteins in the serum and synovial fluid have been shown to be qualitatively identical and the concentrations of the various proteins in synovial fluid have been thought to depend upon the following factors: the molecular weight and the permeability of the synovial membrane which is affected by the degree of inflammation [42,43].…”

Section: Discussionmentioning

confidence: 99%

Prorenin-Renin Axis in Synovial Fluid in Patients with Rheumatoid Arthritis and Osteoarthritis.

et al. 1992

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Abstract. This study was undertaken 1) to determine whether or not renin is present in synovial fluid in patients with rheumatoid arthritis and osteoarthritis, and, if present, 2) to investigate whether it is synthesized in synovial fluid, or it is only transported from the circulation into the synovial cavity. The active renin concentration (indirect) was measured with angiotensin I radioimmunoassay kits. Inactive renin was converted into active renin with Sepharose-bound trypsin. Both active and inactive forms of renin were found in synovial fluid. They were significantly higher in patients with rheumatoid arthritis (n=9) than in those with osteoarthritis (n=16). In plasma, the concentration of inactive renin was significantly higher (P<0.001) in the former. Albumin, transferrin, cr2-macroglobulin, ceruloplasmin and immunoglobulins G and M were also found in synovial fluid. In each disease, a plot of the log ratio of synovial fluid to the serum concentration against the log molecular weight of each protein gave an approximately straight line curve, suggesting that these proteins are derived from the circulation and are transported into the synovial cavity. In contrast, the ratio of synovial fluid to plasma concentrations of active renin was significantly higher than that predicted on the basis of the above-mentioned interrelationships in both diseases, whereas the ratio of inactive renin was significantly lower. These findings suggest that 1) inactive and active renin are filtered into the synovial fluid from thecirculation, and that 2) inactive renin is converted into the active form in the fluid.

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Serum and Synovial Fluid Proteins in Arthritis

Cited by 30 publications

References 10 publications

Permeability of human synovial membrane to plasma proteins. Relationship to molecular size and inflammation

Permeability of human synovial membrane to plasma proteins. Relationship to molecular size and inflammation

Analysis of Serum Proteins and Enzymes Level in Human Subjects with Osteoarthritis

Prorenin-Renin Axis in Synovial Fluid in Patients with Rheumatoid Arthritis and Osteoarthritis.

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