Serum and tissue miRNAs: potential biomarkers for the diagnosis of cervical cancer

Farzanehpour, Mahdieh; Mozhgani, Sayed‐Hamidreza; Jalilvand, Somayeh; Faghihloo, Ebrahim; Akhavan, Setareh; Salimi, Vahid; Azad, Talat Mokhtari

doi:10.1186/s12985-019-1220-y

Cited by 35 publications

(55 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-192-5p could functionally suppressed CSC features in HCC cells through targeting PABPC4 44 . Differently, the levels of serum miR-192-5p was significantly upregulated in nasopharyngeal carcinoma and cervical cancer 43,46 . This controversy indicate that miR-192-5p acts as either tumor-suppressive or oncogenic miRNA in different tumor types.…”

Section: Discussionmentioning

confidence: 85%

“…The genes which were also up-regulated in cervical adenocarcinoma tissues samples were shown. www.nature.com/scientificreports/ Aberrant expression of miR-192-5p has been reported in several tumors, such as hepatocellular carcinoma (HCC), non-small cell lung cancer (NSCLC), pancreatic cancer, and cervical cancer [42][43][44][45] . It was reported that miR-192-5p expression was significantly decreased in HCC, especially in cancer stem cell (CSC) + HCC.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome analysis uncovers the diagnostic value of miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma

Zou

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Despite the fact that the incidence of cervical squamous cell carcinoma has decreased, there is an increase in the incidence of cervical adenocarcinoma. However, our knowledge on cervical adenocarcinoma is largely unclear. Transcriptome sequencing was conducted to compare 4 cervical adenocarcinoma tissue samples with 4 normal cervical tissue samples. mRNA, lncRNA, and miRNA signatures were identified to discriminate cervical adenocarcinoma from normal cervix. The expression of VIL1, HNF1A-AS1, MIR194-2HG, SSTR5-AS1, miR-192-5p, and miR-194-5p in adenocarcinoma were statistically significantly higher than that in normal control samples. The Receiver Operating Characteristic (ROC) curve analysis indicated that combination of miR-192-5p, HNF1A-AS1, and VIL1 yielded a better performance (AUC = 0.911) than any single molecule -and could serve as potential biomarkers for cervical adenocarcinoma. Of note, the combination model also gave better performance than TCT test for cervical adenocarcinoma diagnosis. However, there was no correlation between miR-192-5p or HNF1A-AS1 and HPV16/18 E6 or E7. VIL1 was weakly correlated with HPV18 E7 expression. In summary, our study has identified miR-192-5p/HNF1A-AS1/VIL1 panel that accurately discriminates adenocarcinoma from normal cervix. Detection of this panel may provide considerable clinical value in the diagnosis of cervical adenocarcinoma.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis uncovers the diagnostic value of miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma

Zou

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…After removing 158 duplicate articles, we included the eligible studies through reading titles and abstracts. Ultimately, ten articles [31,[47][48][49][50][51][52][53][54][55], covering 50 studies, were included according to the inclusion and exclusion criteria. Figure 1 showed the flow diagram of study selection.…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, Jia et al found that serum miRNA panel as the diagnostic fingerprint of CC had sensitivity, specificity, and AUC of 88.6%, 81%, and 0.908, respectively [48]. In addition, Farzanehpour et al showed that serum miR-192 can be a potential diagnostic biomarker for early detection of CC, with 83.3% sensitivity, 94.4% specificity, and 0.98 AUC [54]. Luo et al demonstrated that serum miR-3142 was significantly upregulated in CC patients, with the AUC of being 0.935 (95% CI: 0.893-0.977) [65].…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, Aishanjiang et al also found that miR-9 was overexpressed in CC lines and clinical tissues, which can directly target FOXO1 gene to enhance invasion and migration of CC [68]. Moreover, Farzanehpour et al showed that miR-9 had AUC of 0.99 in distinguishing CC patients from healthy individuals, with 100% sensitivity and 94.4% specificity [54]. For miR-205, Xie et al found that elevated miR-205 expression had significantly higher specificity than the high-risk HPV DNA testing, and its sensitivity was similar to the high-risk [51].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Circulating MicroRNAs as a Promising Diagnostic Biomarker for Cervical Intraepithelial Neoplasia and Early Cancer: A Meta-Analysis

Jiang

Hu²,

Zuo

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Background. Cervical cancer (CC) is one of the most common female malignant tumors. And cervical intraepithelial neoplasia (CIN) is the precancerous lesion of CC, which can progress to invasive CC. MicroRNAs (miRNAs) have been found to be potential diagnostic biomarkers for CIN or CC. However, recently, the lack of sufficient studies about the diagnostic value of miRNAs for CIN made it challenging to separately investigate the diagnostic efficacy of miRNAs for CIN. Likewise, the conclusions among those studies were discordant. Therefore, we conducted this meta-analysis, aimed at evaluating the diagnostic efficacy of miRNAs for CIN and CC patients. Methods. Literature search was performed in PubMed, Embase, and Web of Science databases. Pooled sensitivity, specificity, and other diagnostic parameters were calculated through Stata 14.0 software. Furthermore, subgroup analyses and metaregression analysis were conducted to explore the main sources of heterogeneity. Results. Ten articles covering 50 studies were eligible, which included 5,908 patients and 4,819 healthy individuals. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were 0.81 (95% CI, 0.77-0.85), 0.86 (95% CI, 0.83-0.89), 5.9 (95% CI, 4.5-7.7), 0.22 (95% CI, 0.17-0.28), 27 (95% CI, 17-44), and 0.91 (95% CI, 0.88-0.93), respectively. Additionally, the ethnicity and internal reference were the main sources of heterogeneity. Conclusions. Circulating miRNAs can be a promising noninvasive diagnostic biomarker for CIN and early CC, especially miR-9 and miR-205, which need to be verified by large-scale studies.

show abstract

Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer

Dong

Zhuang

Wang

et al. 2021

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

Cervical cancer is the fourth highest mortality cancer among women worldwide.Many researchers have discovered the major anticancer role of miR-192-5p.However, no study has revealed the effect of miR-192-5p on cervical cancer and its molecular mechanism. Therefore, in this study, we aimed to explore the role of miR-192-5p in proliferation, invasion of cervical cancer, and its regulatory mechanism. Firstly, the expression level of miR-192-5p was examined by real-time quantitative polymerase chain reaction. Cell counting kit-8 analysis was applied to detect the proliferation of transfected Caski and SiHa cells. Flow cytometry assay was applied to detect the apoptosis of transfected Caski and SiHa cells. Our result showed that miR-192-5p restrained cervical cancer cell proliferation and induced apoptosis. Then we employed wound healing and transwell assays to analyze the migration and invasion abilities of Caski and SiHa cells in vitro. The results showed that miR-192-5p had an inhibitory effect on cervical cancer migration and invasion. The results of in vivo experiment demonstrated that miR-192-5p also inhibited tumor development in nude mice. We further detected that the binding of transient receptor potential melastatin-subfamily member 7 (TRPM7) to miR-192-5p using bioinformatic methods and dual-luciferase reporter assay. Finally, we found that TRPM7 overexpression reversed the inhibitory effects of miR-192-5p on proliferation, migration, and invasion on cervical cancer cells. In conclusion, the findings of the present study revealed that miR-192-5p performs an inhibitory role in cervical cancer proliferation and invasion by targeting TRPM7.

show abstract

Serum and tissue miRNAs: potential biomarkers for the diagnosis of cervical cancer

Cited by 35 publications

References 60 publications

Transcriptome analysis uncovers the diagnostic value of miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma

Transcriptome analysis uncovers the diagnostic value of miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma

Identification of Circulating MicroRNAs as a Promising Diagnostic Biomarker for Cervical Intraepithelial Neoplasia and Early Cancer: A Meta-Analysis

Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer

Contact Info

Product

Resources

About