Serum Aromatic Microbial Metabolites as Biological Markers in Intensive Care

Белобородова, Н. В.

doi:10.1007/978-3-030-87302-8_64-1

Cited by 2 publications

(4 citation statements)

References 62 publications

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“…In this study, the authors took as a basis the postulate that "function is more important than systematics" [13] and assessed the state of the microbiota by the products of microbial metabolism of aromatic amino acids circulating in the blood. The obtained results confirmed the working hypothesis that patients with aortic aneurysm had deviations in the level of circulating AMMs at the stage of admission to the clinic, before surgery, in comparison with the healthy volunteers.…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, the assessment of the degree of microbiota metabolism disorder may be clinically more significant than information about taxonomic disorders [11,12]. The chapter in the work by Beloborodova N.V., 2022 summarizes the results of the study of microbial metabolites of aromatic structure as markers for monitoring septic conditions in intensive care units [13]. Earlier, an explanation was offered describing an increase in serum concentrations of aromatic amino acid metabolites with its high diagnostic significance.…”

Section: Introductionmentioning

confidence: 99%

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Microbiota Metabolism Failure as a Risk Factor for Postoperative Complications after Aortic Prosthetics

et al. 2023

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Postoperative complications in cardiovascular surgery remain an important unresolved problem, in particular in patients with aortic aneurysm. The role of the altered microbiota in such patients is of great interest. The aim of this pilot study was to determine whether the development of postoperative complications in patients with aortic aneurysm is related with initial or acquired disorders of microbiota metabolism by monitoring the level of some aromatic microbial metabolites (AMMs) circulating in the blood before the surgery and in the early postoperative period. The study comprised patients with aortic aneurysm (n = 79), including patients without complications (n = 36) and patients with all types of complications (n = 43). The serum samples from the patients were collected before and 6 h after the end of the surgery. The most significant results were obtained for the sum of three sepsis-associated AMMs. This level was higher before the surgery in comparison with that of healthy volunteers (n = 48), p < 0.001, and it was also higher in the early postoperative period in patients with all types of complications compared to those without complications, p = 0.001; the area under the ROC curve, the cut-off value, and the odds ratio were 0.7; 2.9 µmol/L, and 5.5, respectively. Impaired microbiota metabolism is important in the development of complications after complex reconstructive aortic surgery, which is the basis for the search for a new prevention strategy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbiota Metabolism Failure as a Risk Factor for Postoperative Complications after Aortic Prosthetics

et al. 2023

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“…These metabolites are absent in the intestines; they do not enter the bloodstream and have no effect on the immune system. On the contrary, in patients with documented bacterial inflammation, hydroxylated phenolic metabolites are always detected in the blood [34,35]. In the case of local forms of infection, the level of hydroxyphenyllactic acid in the serum is within 2-4 µM, and with sepsis, its concentration in the circulation increases and reaches the highest values (up to 10-100 µM or more); that is, it occurs in parallel with the imbalance of various processes in the body of a septic patient [34,35].…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, in patients with documented bacterial inflammation, hydroxylated phenolic metabolites are always detected in the blood [34,35]. In the case of local forms of infection, the level of hydroxyphenyllactic acid in the serum is within 2-4 µM, and with sepsis, its concentration in the circulation increases and reaches the highest values (up to 10-100 µM or more); that is, it occurs in parallel with the imbalance of various processes in the body of a septic patient [34,35]. Thus, the results obtained shed light on the molecular mechanisms of the regulation of the inflammatory process involving the microbiota and its metabolites.…”

Section: Discussionmentioning

confidence: 99%

Influence of Microbiota-Related Metabolites Associated with Inflammation and Sepsis on the Peroxidase Activity of Cyclooxygenase in Healthy Human Monocytes and Acute Monocytic Leukemia Cells

Beloborodova,

Fadeev,

Fedotcheva

2023

IJMS

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The human microbiota produces metabolites that can enter the bloodstream and exert systemic effects on various functions in both healthy and pathological states. We have studied the participation of microbiota-related metabolites in bacterial infection by examining their influence on the activity of cyclooxygenase (COX) as a key enzyme of inflammation. The influence of aromatic microbial metabolites, derivatives of phenylalanine (phenylpropionic acid, PPA), tyrosine (4-hydroxyphenyllactic acid, HPLA), and tryptophan (indolacetic acids, IAA), the concentrations of which in the blood change notably during sepsis, was evaluated. Also, the effect of itaconic acid (ITA) was studied, which is formed in macrophages under the action of bacterial lipopolysaccharides (LPS) and appears in the blood in the early stages of infection. Metabiotic acetyl phosphate (AcP) as a strong acetylating agent was also tested. The activity of COX was measured via the TMPD oxidation colorimetric assay using the commercial pure enzyme, cultured healthy monocytes, and the human acute monocytic leukemia cell line THP-1. All metabolites in the concentration range of 100–500 μM lowered the activity of COX. The most pronounced inhibition was observed on the commercial pure enzyme, reaching up to 40% in the presence of AcP and 20–30% in the presence of the other metabolites. On cell lysates, the effect of metabolites was preserved, although it significantly decreased, probably due to their interaction with other targets subject to redox-dependent and acetylation processes. The possible contribution of the redox-dependent action of microbial metabolites was confirmed by assessing the activity of the enzyme in the presence of thiol reagents and in model conditions, when the COX-formed peroxy intermediate was replaced with tert-butyl hydroperoxide (TBH). The data show the involvement of the microbial metabolites in the regulation of COX activity, probably due to their influence on the peroxidase activity of the enzyme.

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Serum Aromatic Microbial Metabolites as Biological Markers in Intensive Care

Cited by 2 publications

References 62 publications

Microbiota Metabolism Failure as a Risk Factor for Postoperative Complications after Aortic Prosthetics

Microbiota Metabolism Failure as a Risk Factor for Postoperative Complications after Aortic Prosthetics

Influence of Microbiota-Related Metabolites Associated with Inflammation and Sepsis on the Peroxidase Activity of Cyclooxygenase in Healthy Human Monocytes and Acute Monocytic Leukemia Cells

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