Serum biochemical changes in rabbits on a regular diet with and without flax lignan complex following a high-cholesterol diet

Prasad, Kailash

doi:10.1055/s-0031-1278276

Cited by 12 publications

(8 citation statements)

References 14 publications

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“…Similar conclusions have been reported by Zhou et al ( 51 ), when Sprague–Dawley rats were fed high-amylose transgenic rice for up to three generations. The findings of this study corroborate with that of Prasad ( 52 ) who fed rabbits with a high-cholesterol diet for 6-months. However, on the contrary ( 42 ), reported significant changes in the levels of serum electrolytes of rabbits following 6-weeks consumption of dietary fumonisin.…”

Section: Discussionsupporting

confidence: 91%

Nutritional Assessment of Dietary Bt and Cp4EPSPS Proteins on the Serum Biochemical Changes of Rabbits at Different Developmental Stages

et al. 2018

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In recent years, the influence of genetically modified (GM) cotton expressing different types of Bt and EPSPS genes has been attested in term of reduced application of pesticides and insecticides coupled with improved cotton production. Although the cultivation of GM cotton has been authorized by the regulatory authorities of various countries in the world, based on the biosafety studies reported by most of the GM cotton producers, yet the information on its safe use are inadequate. In order to support the issues on food safety, it is therefore mandatory to conduct further safety assessment studies on GM cotton for each independent transgenic event on the basis of case assessment rule. In the present study, the effects of different doses of dietary GM cotton seed expressing Bt and EPSPS genes were studied on the level of serum biochemical in albino rabbits (Oryctolagus cuniculus). The rabbits were fed a diet containing different levels of GM cotton seeds (i.e., 20, 30, and 40% w/w) respectively mixed with standard diet for 180 days. During the course of the study, various serum enzymes, electrolytes, proteins, glucose and serum total cholesterol were examined at specific time intervals (0, 45, 90, 135, and 180) days. The results showed non-significant (P > 0.05) dose dependent effects in most of the evaluated serum biochemical parameters. Although, the results in some of the serum biochemistry were significantly different (P < 0.05) among the groups, however, they were not biologically significant, since all the differences were within the normal physiological range. These results thus, suggested that the GM cotton seed meal could be considered as safe as other conventional feed ingredients. The experimental evidence for the safe usage of GM cotton was highlighted in this study.

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Section: Discussionsupporting

confidence: 91%

Nutritional Assessment of Dietary Bt and Cp4EPSPS Proteins on the Serum Biochemical Changes of Rabbits at Different Developmental Stages

et al. 2018

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“… a Reference values for these enzymes in male rabbits are 35–60 U/L for AST, 22–51 U/L for ALT (Loeb and Quimby, 1989), and 10–12 U/L for GGT (Prasad, 2008). Normal values for SDH in rabbits were unavailable …”

Section: Resultsmentioning

confidence: 99%

Development of Dimethandrolone 17 -Undecanoate (DMAU) as an Oral Male Hormonal Contraceptive: Induction of Infertility and Recovery of Fertility in Adult Male Rabbits

Attardi

Engbring

Gropp

et al. 2010

Journal of Andrology

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Dimethandrolone undecanoate (DMAU: 7a,11b-dimethyl-19-nortestosterone 17b-undecanoate) is a potent orally active androgen with progestational activity that is in development for therapeutic uses in men. We hypothesized that because of its dual activity, DMAU might have potential as a single-agent oral hormonal contraceptive. To test this possibility, adult male rabbits (5/ group) of proven fertility were treated orally with vehicle or DMAU at 1.0, 2.5, 5.0, or 10.0 mg/kg/d for 12 or 13 weeks. Semen and blood samples were collected every other week through week 30. Sperm were decreased (P , .05) in semen samples from DMAU-treated rabbits at 2.5 and 5.0 mg/kg/d at weeks 12, 14, 16, 18, and 20 compared to week 0 (prior to treatment). The percentage of forward progressive motile sperm in those rabbits that still had measurable sperm was also reduced by DMAU treatment at 2.5 mg/kg/d at weeks 14, 16, 18, and 20 and at 5.0 mg/kg/d at week 18 (P , .05). At 1.0 mg/kg/d only 1 rabbit had reduced sperm numbers and motility. A mating trial was performed at week 15. The number of bred males that were fertile was 4 of 4 in the vehicle-treated group and 4 of 5, 0 of 4, and 2 of 5 in the 1.0, 2.5, and 5.0 mg/kg/d DMAU treatment groups. By week 22, sperm numbers and forward progressive motility increased, and they returned to pretreatment levels in all DMAU-treated rabbits by week 30. All bred males were fertile at week 31. Serum levels of testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were significantly suppressed in DMAU (1.0, 2.5, or 5.0 mg/kg/d)-treated rabbits during the 12-week dosing interval, but were comparable to pretreatment levels after cessation of dosing. These data indicate that DMAU suppressed the hypothalamic-pituitary-gonadal axis, resulting in severe oligospermia in the majority of rabbits in the 2.5 and 5.0 mg/kg/d dosing groups. Infertility was observed when sperm numbers decreased to about 10% of pretreatment levels. In rabbits dosed with DMAU at 10.0 mg/ kg/d, no effect on sperm numbers or motility was observed by week 12. Dosing continued for another week, and the rabbits underwent a gross necropsy on week 13 with removal of testes and epididymides for histology and preparation of testicular cytosol. Serum testosterone, FSH, and LH levels were considerably suppressed in these rabbits as in the lower-dose groups. The lack of oligospermia in the 10.0 mg/kg group as well as in the 2 fertile males in the 5.0 mg/kg group may have been due to high intratesticular levels of 7a,11b-dimethyl-19-nortestosterone, the active metabolite of DMAU. Hence, as observed previously for testosterone, DMAU has a biphasic effect on spermatogenesis. Collectively, these data indicate that DMAU has the potential to be an orally active single-agent male hormonal contraceptive at an appropriate dose level and should be tested for contraceptive efficacy in nonhuman primates.

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“…This was an indication that administration of ambrex to the rats prevents leakage of these enzymes and restores the integrity of cell membrane in the liver. The reason behind the decline in glucose level in Atorvastatin treated group animals was uncertain but it could be that high serum cholesterol elevates the level of glucan-like peptide-1 which augments insulin secretion from the β cells of pancreas that results in hypoglycemia (Xu et al, 1949;Prasad, 2008). Table 3 shows that urea and creatinine are reduced in treated animals as compared to Group B animals.…”

Section: Discussionmentioning

confidence: 99%

Antihyperlipidemic effect of ambrex, a polyherbal formulation against experimentally induced hypercholesterolemia in rats

Devi¹,

Rajkumar²

2013

Afr. J. Pharm. Pharmacol.

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Ambrex, a polyherbal formulation was tested for its antihypercholesterolemic effect against experimentally induced hypercholesterolemia in rats. Alteration in the levels of serum marker enzymes, alanine amino transferase (ALT), aspartate amino transferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and general biochemical parameters glucose, protein, urea and creatinine were tested in both treated and untreated groups. Rats treated with high fat diet for four weeks exhibited hypercholesterolemia. Oral administration of ambrex at a dose of 40 mg/kg body weight significantly decreased serum total cholesterol, triglycerides and low density lipoproteins (LDL) levels and there was a concomitant increase in serum high density lipoprotein (HDL) levels. Atherogenic index of the ambrex treated group showed marked improvement compared to other groups. Studies established that ambrex might be a safe drug that exhibits negligible toxic effects on liver and kidney functions under experimental condition. The histopathalogical observation of liver tissue also confirms the same. The results shows the ambrex possess considerable antihypercholesterolemic activity compared with standard drug, atorvastatin.

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Serum biochemical changes in rabbits on a regular diet with and without flax lignan complex following a high-cholesterol diet

Abstract: Short-term use of a high-cholesterol diet, or a regular diet with or without FLC following the high-cholesterol diet, does not produce deleterious effects in the liver, kidneys, skeletal muscle, intestine or bone, as shown by changes in serum electrolytes, glucose and enzymes.

Cited by 12 publications

References 14 publications

Nutritional Assessment of Dietary Bt and Cp4EPSPS Proteins on the Serum Biochemical Changes of Rabbits at Different Developmental Stages

Nutritional Assessment of Dietary Bt and Cp4EPSPS Proteins on the Serum Biochemical Changes of Rabbits at Different Developmental Stages

Development of Dimethandrolone 17 -Undecanoate (DMAU) as an Oral Male Hormonal Contraceptive: Induction of Infertility and Recovery of Fertility in Adult Male Rabbits

Antihyperlipidemic effect of ambrex, a polyherbal formulation against experimentally induced hypercholesterolemia in rats

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