Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Liu, Zhao-Yan; Yishake, Dinuerguli; Fang, Aiping; Zhang, Daoming; Liao, Gong‐Cheng; Tan, Xuying; Zhang, Yaojun; Zhu, Huilian

doi:10.21203/rs.2.19868/v1

Cited by 5 publications

(5 citation statements)

References 22 publications

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“…27 Choline is an essential nutrient in all cells to maintain the stability of cell membrane structure, 28 and higher plasma Choline levels were associated with better HCC survival rates. 29 Choline of HCC plasma was lower than liver cirrhosis and healthy control group, 11 which is consistent with our results. L-Pyroglutamic acid is also called 5-oxoproline, a synthetic substrate of glutathione (GSH); its reduction will weaken hepatic antioxidant capacity.…”

Section: Discussionsupporting

confidence: 90%

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Mei¹,

Liu²,

Tang³

et al. 2022

JHC

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Purpose To clarify the underlying regulatory mechanisms of progression from liver cirrhosis to hepatocellular carcinoma (HCC), we analyzed the microbiomics, metabolomics, and proteomics in plasma and tissues from patients with HCC or decompensated liver cirrhosis (DC). Patients and Methods Tissues and plasma from 44 HCC patients and 28 patients with DC were collected for metabolomic analysis. 16S rRNA sequencing was performed in nine HCC tissues (HCCT), four distal noncancerous tissues (HCCN), and 11 DC tissues (DCT). Five HCC tissues had liver cirrhosis (HCCT-LC). Five hepatocellular carcinoma tissues without liver cirrhosis (HCCT-NLC) and five DCT were selected for proteomic sequencing. After combining proteomic and metabolomic analysis, we constructed a mouse model of chronic liver injury using carbon tetrachloride (CCl4) and treated them with vitamin B6 (VB6). Results 16s rRNA sequence results showed that HCC tissues had higher alpha diversity. The highest LDA scores were detected for Elizabethkingia in HCCT, Subsaxibacter in DCT, and Stenotrophomon in HCCN. Metabolomics results demonstrated some metabolites, including capric acid, L-threonate, choline, alpha-D-Glucose, D-ribose, betaine, 2E-eicosenoic acid, linoleic acid, L-palmitoylcarnitine, taurodeoxycholic acid, L-pyroglutamic acid, androsterone sulfate, and phthalic acid mono-2-ethylhexyl ester (MEHP), had better diagnostic efficacy than AFP (AUC: 0.852; 95% CI: 0.749, 0.954). In a combined analysis of metabolomics and proteomics, we found that HCCT-LC had more obvious disorders of VB6 metabolism and pentose and glucuronate interconversions than DCT, and kynurenine metabolism disorder was more significant in HCCT-LC than in HCCT-NLC. In the CCl4-induced chronic liver injury model, after VB6 supplementation, inflammatory cell infiltration, hepatocyte edema, and degeneration were significantly improved. Conclusion We found significant differences in the flora distribution between HCCT and DC; MEHP was a new diagnostic biomarker of HCC, and VB6 ameliorated the inflammatory cell infiltration, hepatocyte edema, and degeneration in chronic liver injury.

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Section: Discussionsupporting

confidence: 90%

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Mei¹,

Liu²,

Tang³

et al. 2022

JHC

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“…[139][140][141][142] Choline depletion affects lipid metabolism and transport. 143 Jiang et al found that choline supplementation increases global DNA methylation and the expression of peroxisomal acyl-coenzyme A oxidase 1, which mediates FA β-oxidation. 144 Liu et al showed that higher choline intake can improve the overall general health.…”

Section: Methionine Metabolism and Cldsmentioning

confidence: 99%

Methionine metabolism in chronic liver diseases: an update on molecular mechanism and therapeutic implication

Wang

Liang

et al. 2020

Sig Transduct Target Ther

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As one of the bicyclic metabolic pathways of one-carbon metabolism, methionine metabolism is the pivot linking the folate cycle to the transsulfuration pathway. In addition to being a precursor for glutathione synthesis, and the principal methyl donor for nucleic acid, phospholipid, histone, biogenic amine, and protein methylation, methionine metabolites can participate in polyamine synthesis. Methionine metabolism disorder can aggravate the damage in the pathological state of a disease. In the occurrence and development of chronic liver diseases (CLDs), changes in various components involved in methionine metabolism can affect the pathological state through various mechanisms. A methionine-deficient diet is commonly used for building CLD models. The conversion of key enzymes of methionine metabolism methionine adenosyltransferase (MAT) 1 A and MAT2A/MAT2B is closely related to fibrosis and hepatocellular carcinoma. In vivo and in vitro experiments have shown that by intervening related enzymes or downstream metabolites to interfere with methionine metabolism, the liver injuries could be reduced. Recently, methionine supplementation has gradually attracted the attention of many clinical researchers. Most researchers agree that adequate methionine supplementation can help reduce liver damage. Retrospective analysis of recently conducted relevant studies is of profound significance. This paper reviews the latest achievements related to methionine metabolism and CLD, from molecular mechanisms to clinical research, and provides some insights into the future direction of basic and clinical research.

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“…The apparatus was used for the rapid quantification of the blood-borne metabolite choline in human plasma (physiological range 7 -20 µM [23]). Elevated concentrations of choline have been associated with several conditions including cardiovascular disease [24] and cancer [25]. We demonstrate that the hybrid device can perform multiple measurements with a LOD of 3.2 µM and resolution of 1.6 µM.…”

Section: Micromolar Metabolite Measurement In Anmentioning

confidence: 95%

Micromolar Metabolite Measurement in an Electronically Multiplexed Format

Annese

Giagkoulovits

et al. 2022

IEEE Trans. Biomed. Eng.

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The detection of metabolites such as choline in blood are important in clinical care for patients with cancer and cardiovascular disease. Choline is only present in human blood at low concentrations hence accurate measurement in an affordable point-ofcare format is extremely challenging. Although complementary metal-oxide semiconductor (CMOS) and microfluidics are individually mature technologies, their integration has presented challenges that we overcome in a novel, cost-effective, single-step process.Methods: To demonstrate the process, we present the microfluidic integration of a metabolomics-on-CMOS point-of-care platform with four capillary microfluidic channels on top of a CMOS optical sensor array.Results: The fabricated device was characterised to verify the required structural profile, mechanical strength, optical spectra, and fluid flow. As a proof of concept, we used the device for the in-vitro quantification of choline in human blood plasma with a limit of detection of 3.2 µM and a resolution of 1.6 µM.Significance: Integration of microfluidics on to CMOS technology has the potential to enable advanced sensing technologies with extremely low limit of detection that are well suited to multiple clinical metabolite measurements.

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Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Cited by 5 publications

References 22 publications

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Methionine metabolism in chronic liver diseases: an update on molecular mechanism and therapeutic implication

Micromolar Metabolite Measurement in an Electronically Multiplexed Format

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