Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Liu, Zhao-Yan; Yishake, Dinuerguli; Fang, Aiping; Zhang, Daoming; Liao, Gong‐Cheng; Tan, Xuying; Zhang, Yaojun; Zhu, Huilian

doi:10.1186/s12986-020-00445-z

Cited by 13 publications

(6 citation statements)

References 30 publications

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“… 27 Choline is an essential nutrient in all cells to maintain the stability of cell membrane structure, 28 and higher plasma Choline levels were associated with better HCC survival rates. 29 Choline of HCC plasma was lower than liver cirrhosis and healthy control group, 11 which is consistent with our results. L-Pyroglutamic acid is also called 5-oxoproline, a synthetic substrate of glutathione (GSH); its reduction will weaken hepatic antioxidant capacity.…”

Section: Discussionsupporting

confidence: 90%

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Mei¹,

Liu²,

Tang³

et al. 2022

JHC

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Purpose To clarify the underlying regulatory mechanisms of progression from liver cirrhosis to hepatocellular carcinoma (HCC), we analyzed the microbiomics, metabolomics, and proteomics in plasma and tissues from patients with HCC or decompensated liver cirrhosis (DC). Patients and Methods Tissues and plasma from 44 HCC patients and 28 patients with DC were collected for metabolomic analysis. 16S rRNA sequencing was performed in nine HCC tissues (HCCT), four distal noncancerous tissues (HCCN), and 11 DC tissues (DCT). Five HCC tissues had liver cirrhosis (HCCT-LC). Five hepatocellular carcinoma tissues without liver cirrhosis (HCCT-NLC) and five DCT were selected for proteomic sequencing. After combining proteomic and metabolomic analysis, we constructed a mouse model of chronic liver injury using carbon tetrachloride (CCl4) and treated them with vitamin B6 (VB6). Results 16s rRNA sequence results showed that HCC tissues had higher alpha diversity. The highest LDA scores were detected for Elizabethkingia in HCCT, Subsaxibacter in DCT, and Stenotrophomon in HCCN. Metabolomics results demonstrated some metabolites, including capric acid, L-threonate, choline, alpha-D-Glucose, D-ribose, betaine, 2E-eicosenoic acid, linoleic acid, L-palmitoylcarnitine, taurodeoxycholic acid, L-pyroglutamic acid, androsterone sulfate, and phthalic acid mono-2-ethylhexyl ester (MEHP), had better diagnostic efficacy than AFP (AUC: 0.852; 95% CI: 0.749, 0.954). In a combined analysis of metabolomics and proteomics, we found that HCCT-LC had more obvious disorders of VB6 metabolism and pentose and glucuronate interconversions than DCT, and kynurenine metabolism disorder was more significant in HCCT-LC than in HCCT-NLC. In the CCl4-induced chronic liver injury model, after VB6 supplementation, inflammatory cell infiltration, hepatocyte edema, and degeneration were significantly improved. Conclusion We found significant differences in the flora distribution between HCCT and DC; MEHP was a new diagnostic biomarker of HCC, and VB6 ameliorated the inflammatory cell infiltration, hepatocyte edema, and degeneration in chronic liver injury.

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Section: Discussionsupporting

confidence: 90%

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Mei¹,

Liu²,

Tang³

et al. 2022

JHC

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“…Two large US cohort studies revealed positive associations of choline intake with all-cause and CVD mortality [ 14 ], but a prospective study of Japanese adults showed no such associations [ 15 ]. Reversely, a large prospective cohort study revealed that higher serum choline levels at diagnosis were associated with better hepatocellular carcinoma survival outcomes [ 16 ]. Hence, in this large, prospective, follow-up study of the CSPPT, we performed a nested case–control study to assess the prospective association between the baseline serum choline concentrations and all-cause mortality risk in adults with hypertension, and further evaluated whether the associations were modified by sociodemographic and clinical characteristics and lifestyle factors.…”

Section: Introductionmentioning

confidence: 99%

Association of serum choline levels and all-cause mortality risk in adults with hypertension: a nested case–control study

Song

Liang

et al. 2021

Nutr Metab (Lond)

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Background Serum choline levels were associated with multiple chronic diseases. However, the association between serum choline and all-cause mortality in Chinese adults with hypertension remains unclear. The purpose of this study is to explore the association between serum choline concentrations and all-cause mortality risk in Chinese adults with hypertension, a high-risk population. Methods A nested, case–control study was conducted that included 279 patients with all-cause death, and 279 matched, living controls, derived from the China Stroke Primary Prevention Trial (CSPPT). Baseline serum choline concentrations were measured by liquid chromatography with tandem quadrupole mass spectrometry (LC–MS/MS). Multivariate logistic regression analysis was used to assess the association of serum choline levels and all-cause mortality risk, with adjustment of pertinent covariables, including folic acid and homocysteine. Results The median age of all participants was 64.13 years [interquartile range (IQR), 57.33–70.59 years]. The median serum choline concentration for cases (9.51 μg/mL) was higher than that in controls (7.80 μg/mL) (P = 0.009). When serum choline concentration was assessed as a continuous variable (per SD increased), there was a positive relation between serum choline levels and all-cause mortality risk [odds ratios (OR), 1.29; 95% confidence intervals (95%CI), 1.06–1.57; P = 0.010]. There was an increased all-cause mortality risk for participants in quartiles 2–4 (≥ 4.00 μg/mL; OR, 1.79; 95%CI, 1.15–2.78 compared with quartile 1 (< 4.00 μg/mL). In addition, non-drinking was found to promote the incidence of all-cause mortality for those with high choline concentrations. Conclusions High serum choline concentrations were associated with increased all-cause mortality risk among Chinese adults with hypertension, compared to lower choline concentrations. Trial registration clinicaltrials.gov Identifier: NCT007948885; UTL: https://clinicaltrials.gov/ct2/show/NCT00794885?term=NCT00794885&draw=2&rank=1.

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“…Amino acids are used for the synthesis of tissue proteins and hormones and serve as energy-producing substances in organisms [ 27 ]. Phosphatidylcholine is an significant component of cell membranes and plays an important role in cell signaling pathways [ 28 , 29 ]. Acetyl-CoA plays an essential role in glycolysis and fatty acid oxidation [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Candidate Genes Involved in Light-Induced Primordium Differentiation in Pleurotus eryngii

et al. 2021

IJMS

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Pleurotus eryngii, a highly valued edible fungus, is one of the major commercially cultivated mushrooms in China. The development of P. eryngii, especially during the stage of primordium differentiation, is easily affected by light. However, the molecular mechanism underlying the response of primordium differentiation to light remains unknown. In the present study, primordium expression profiles under blue-light stimulation, red-light stimulation, and exposure to darkness were compared using high-throughput sequencing. A total of 16,321 differentially expressed genes (DEGs) were identified from three comparisons. GO enrichment analysis showed that a large number of DEGs were related to light stimulation and amino acid biosynthesis. KEGG analyses demonstrated that the MAPK signaling pathway, oxidative phosphorylation pathway, and RNA transport were most active during primordium differentiation. Furthermore, it was predicted that the blue-light photoreceptor WC-1 and Deoxyribodipyrimidine photolyase PHR play important roles in the primordium differentiation of P. eryngii. Taken together, the results of this study provide a speculative mechanism that light induces primordium differentiation and a foundation for further research on fruiting body development in P. eryngii.

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Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Cited by 13 publications

References 30 publications

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Association of serum choline levels and all-cause mortality risk in adults with hypertension: a nested case–control study

Transcriptome Analysis Reveals Candidate Genes Involved in Light-Induced Primordium Differentiation in Pleurotus eryngii

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