Serum Creatine Kinase B Concentrations in Acute Cerebrovascular Diseases

Pfeiffer, Frederick E.; Homburger, Henry A.; Yanagihara, Takehiko

doi:10.1001/archneur.1984.04050220073017

Cited by 23 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CK in the isoforms of CK-MM and CK-MB have been widely studied for the diagnosis of myocardial infarction 22 , but less is known about CK-BB disease relevance. In concordance with our results, CK-BB has been well reported to immediately increase in blood circulation and CSF following acute cerebral injuries, including cerebrovascular traumatisms, meningitis and strokes 23 – 25 , and its role as a plausible diagnostic biomarker for ischemic stroke has been previously postulated 26 , 27 . CK-BB provenance has been slightly controversial; some studies have proven that astrocytes and neurons are the main CK-BB source, whereas others, including us, could only proof that astrocytes express CK-BB in brain 28 .…”

Section: Discussionsupporting

confidence: 92%

Characterization of the rat cerebrospinal fluid proteome following acute cerebral ischemia using an aptamer-based proteomic technology

Simats

García‐Berrocoso

Ramiro

et al. 2018

Sci Rep

View full text Add to dashboard Cite

The limited accessibility to the brain has turned the cerebrospinal fluid (CSF) into a valuable source that may contribute to the complete understanding of the stroke pathophysiology. Here we have described the CSF proteome in the hyper-acute phase of cerebral ischemia by performing an aptamer-based proteomic assay (SOMAscan) in CSF samples collected before and 30 min after male Wistar rats had undergone a 90 min Middle Cerebral Artery Occlusion (MCAO) or sham-surgery. Proteomic results indicated that cerebral ischemia acutely increased the CSF levels of 716 proteins, mostly overrepresented in leukocyte chemotaxis and neuronal death processes. Seven promising candidates were further evaluated in rat plasma and brain (CKB, CaMK2A, CaMK2B, CaMK2D, PDXP, AREG, CMPK). The 3 CaMK2 family-members and CMPK early decreased in the infarcted brain area and, together with AREG, co-localized with neurons. Conversely, CKB levels remained consistent after the insult and specifically matched with astrocytes. Further exploration of these candidates in human plasma revealed the potential of CKB and CMPK to diagnose stroke, while CaMK2B and CMPK resulted feasible biomarkers of functional stroke outcome. Our findings provided insights into the CSF proteome following cerebral ischemia and identified new outstanding proteins that might be further considered as potential biomarkers of stroke.

show abstract

Section: Discussionsupporting

confidence: 92%

Characterization of the rat cerebrospinal fluid proteome following acute cerebral ischemia using an aptamer-based proteomic technology

Simats

García‐Berrocoso

Ramiro

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The brain type creatine kinase is the predominant form in the brain, but it is present in smaller quantities in other tissues mixed with the MM and MB isoenzymes [10][11]. Pfeiffer et al [7] reported the peak within 24 hours of clinical onset as a reflection of tissue destruction in cerebral infarction; Phillips et al [13] observed the highest level after 48 hours, possibly when hypoxic cells developed structural changes due to "maturation" of ischemia. Increased BBCK CSF concentration was found in various other neurologic diseases, but the level does not appear elevated in the case of inactive, chronic or degenerative disease [6].…”

Section: Discussionmentioning

confidence: 99%

Creatine kinase BB activity in serum of patients with acute stroke: correlation with the severity of brain damage

et al. 1987

View full text Add to dashboard Cite

BB-CK activity was measured in 11 patients with stroke and in 10 controls. Blood samples were taken 36 hours after the clinical stroke onset in every patient. Sera were stored at -80 degrees and analyzed within two months. The creatine kinase isoenzymatic pattern was determined by ion-exchange column separation and gradient elution system. The mean BB-CK concentration in patients with stroke was significantly higher than in controls (p less than 0.01). In the group of "stroke" patients we found a correlation between severity of brain damage, as suggested by the clinical picture and CT scans, and serum values of BB-CK.

show abstract

“…With any destructive process of these tissues, the enzyme leaks into extra cellular fluids and then into body fluids [10]. Hence, accurate separation and measurement of these isoenzymes in serum has become more important in the diagnosis and prognosis of neurological diseases [13]. Oxidative modification of brain proteins may disturb neuronal functions by Author for correspondence: Jagadeesan Arunakaran, Department of Endocrinology, University of Madras, Chennai, India (e-mail j_arunakaran@hotmail.com).…”

mentioning

confidence: 99%

Oxidative Stress Alters Creatine Kinase System in Serum and Brain Regions of Polychlorinated Biphenyl (Aroclor 1254)‐Exposed Rats: Protective Role of Melatonin

Venkataraman

Giribabu

Selvakumar

et al. 2009

Basic Clin Pharma Tox

View full text Add to dashboard Cite

Polychlorinated biphenyls are one of the environmental toxicants and neurotoxic compounds which induce the production of free radicals. Creatine kinase plays a key role in energy metabolism of nervous tissue and might be one of the targets for reactive oxygen species. Melatonin, an indoleamine, plays an important role in neurodegenerative diseases as an antioxidant and neuroprotector. The objective of the present study was to investigate the protective role of melatonin on polychlorinated biphenyl (Aroclor 1254)-induced oxidative stress and the changes in creatine kinase activity in brain regions of adult rats. Group I: rats were intraperitoneally (i.p.) administered with corn oil (vehicle) for 30 days. Group II: rats injected i.p. with Aroclor 1254 at 2 mg/kg body weight (bw)/day for 30 days. Groups III and IV: rats i.p. received melatonin (5 or 10 mg/kg bw/day) simultaneously with Aroclor 1254 for 30 days. After 30 days, rats were killed and the brain regions were dissected to cerebral cortex, cerebellum and hippocampus. Lipid peroxidation, hydroxyl radical and hydrogen peroxide (H 2 O 2 ) levels were determined. The activity of creatine kinase was assayed in serum and brain regions, and its isoenzymes in serum were separated electrophoretically. Activity of creatine kinase was decreased while an increase in H 2 O 2 , hydroxyl radical and lipid peroxidation was observed in brain regions of polychlorinated biphenyl-treated rats. Also polychlorinated biphenyl exposure showed a significant increase in serum creatine kinase level and its isoforms such as BB-creatine kinase, MB-creatine kinase, and MM-creatine kinase. Administration of melatonin prevented these alterations induced by polychlorinated biphenyl by its free radical scavenging mechanism. Thus, polychlorinated biphenyl alters creatine kinase activity by inducing oxidative stress in brain regions, which can be protected by melatonin.

show abstract

Serum Creatine Kinase B Concentrations in Acute Cerebrovascular Diseases

Cited by 23 publications

References 18 publications

Characterization of the rat cerebrospinal fluid proteome following acute cerebral ischemia using an aptamer-based proteomic technology

Characterization of the rat cerebrospinal fluid proteome following acute cerebral ischemia using an aptamer-based proteomic technology

Creatine kinase BB activity in serum of patients with acute stroke: correlation with the severity of brain damage

Oxidative Stress Alters Creatine Kinase System in Serum and Brain Regions of Polychlorinated Biphenyl (Aroclor 1254)‐Exposed Rats: Protective Role of Melatonin

Contact Info

Product

Resources

About