Serum elastase 1 in inflammatory pancreatic and gastrointestinal diseases and in renal insufficiency. A comparison with other serum pancreatic enzymes

Malfertheiner, Peter; Büchler, Markus W.; Stănescu, Anca Daniela; Uhl, Waldemar; Ditschuneit, H.

doi:10.1007/bf02788214

Cited by 26 publications

(1 citation statement)

References 25 publications

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“…Indeed, a Phe residue in P1 has been shown to be correlated to a strong interaction with Chymotrypsin with an association constant value of 2.5 × 10 9 M −1 [ 59 ]. These Kunitz-Type proteins could be promising inhibitors of Elastase-like enzymes that are involved in important diseases such as acute pancreatitis, chronic inflammatory lung diseases, and cancer [ 60 , 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Kunitz-Type Protease Inhibitors from Blister Beetles (Coleoptera, Meloidae)

et al. 2022

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Protease inhibitors are widely studied since the unrestricted activity of proteases can cause extensive organ lesions. In particular, elastase activity is involved in the pathophysiology of acute lung injury, for example during SARS-CoV-2 infection, while serine proteases and thrombin-like proteases are involved in the development and/or pathology of the nervous system. Natural protease inhibitors have the advantage to be reversible and with few side effects and thus are increasingly considered as new drugs. Kunitz-type protease inhibitors (KTPIs), reported in the venom of various organisms, such as wasps, spiders, scorpions, and snakes, have been studied for their potent anticoagulant activity and widespread protease inhibitor activity. Putative KTPI anticoagulants have been identified in transcriptomic resources obtained for two blister beetle species, Lydus trimaculatus and Mylabris variabilis. The KTPIs of L. trimaculatus and M. variabilis were characterized by combined transcriptomic and bioinformatics methodologies. The full-length mRNA sequences were divided on the base of the sequence of the active sites of the putative proteins. In silico protein structure analyses of each group of translational products show the biochemical features of the active sites and the potential protease targets. Validation of these genes is the first step for considering these molecules as new drugs for use in medicine.

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